JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on May 23, 2003
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
278/22/20133    most recent
M210855200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kokura, K.
Right arrow Articles by Ishii, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kokura, K.
Right arrow Articles by Ishii, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print March 19, 2003
J. Biol. Chem, 10.1074/jbc.M210855200
Submitted on October 23, 2002
Revised on February 20, 2003
Accepted on March 19, 2003

The Ski-binding protein C184M negatively regulates TGF-beta signaling by sequestering the Smad proteins in the cytoplasm

Kenji Kokura, Hyungtae Kim, Toshie Shinagawa, Md Matiullah Khan, Teruaki Nomura, and Shunsuke Ishii

Laboratory of Molecular Genetics, RIKEN Tsukuba Institute, Tsukuba, Ibaraki 305-0074

Corresponding Author: sishii{at}rtc.riken.go.jp

Ski is a transcriptional co-repressor, and is involved in the negative regulation of TGF-beta signaling. To understand more fully the role of Ski in TGF-beta signaling, we searched for novel Ski-interacting proteins. The identified C184M protein consists of 189 amino acids and contains the leucine-rich (LR) region. An association between Ski and C184M involving the LR region of C184M and the C-terminal coiled-coil motif of Ski was confirmed by GST pull-down and immunoprecipitation assays. The C184M protein is located in the cytosol, and the C184M and Ski signals co-localized in the cytoplasm when C184M was co-expressed with Ski in CV-1 cells. The cytoplasmic C184M-Ski complex inhibited the nuclear translocation of Smad2. Consistent with this, the activity of promoter containing the Smad-binding sites was repressed by C184M, and the TGF-beta-induced growth inhibition of mink lung Mv1Lu cells was attenuated by the ectopic expression of C184M. Thus, C184M inhibits TGF-beta signaling in concert with Ski. In hepatocytes, which express significant levels of C184M, the Ski signals were found only in the cytoplasm, supporting the notion that C184M forms a complex with Ski in the cytosol.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 GENES CELLSHome page
M. Nagata, K. Goto, S. Ehata, N. Kobayashi, M. Saitoh, H. Miyoshi, T. Imamura, K. Miyazawa, and K. Miyazono
Nuclear and cytoplasmic c-Ski differently modulate cellular functions
Genes Cells, November 1, 2006; 11(11): 1267 - 1280.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Prunier, M. Pessah, N. Ferrand, S. R. Seo, P. Howe, and A. Atfi
The Oncoprotein Ski Acts as an Antagonist of Transforming Growth Factor-{beta} Signaling by Suppressing Smad2 Phosphorylation
J. Biol. Chem., July 3, 2003; 278(28): 26249 - 26257.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.