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M211591200v1
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Papers In Press, published online ahead of print May 23, 2003
J. Biol. Chem, 10.1074/jbc.M211591200
Submitted on November 13, 2002
Revised on May 21, 2003
Accepted on May 22, 2003

Cloning and characterization of hCTF18, hCTF8, and hDCC1: human homologs of an S. cerevisiae complex involved in sister chromatid cohesion establishment

Carolin J. Merkle, Larry M. Karnitz, John T. Henry-Sánchez, and Junjie Chen

Department of Oncology Research, Mayo Clinic, Rochester, MN 55905

Corresponding Author: chen.junjie{at}mayo.edu

A growing body of evidence suggests that establishment of sister chromatid cohesion is dependent on replication fork passage over a precohesion area. In S. cerevisiae, this process involves an alternative replication factor C (RFC) complex which contains the four small RFC subunits as well as CTF18, CTF8 and DCC1. Here, we show that an evolutionarily conserved homologous complex exists in the nucleus of human cells. We demonstrate that hCTF18, hCTF8 and hDCC1 interact with each other as well as with the p38 subunit of RFC. This alternative RFC-containing complex interacts with PCNA, but not with the Rad9/Rad1/Hus1 complex, a PCNA-like clamp involved in the DNA damage response. hCTF18 preferentially binds chromatin during S phase, suggesting a role during replication. Our data provide evidence for the existence of an alternative RFC complex with a probable role in mammalian cohesion establishment.


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