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Papers In Press, published online ahead of print February 7, 2003
CABM, University of Med and Dent of New Jersey, Piscataway, NJ 08854
Corresponding Author: shatkin{at}cabm.rutgers.edu
ABSTRACT Capping of the initiated 5' ends of RNA polymerase II products is evolutionarily and functionally conserved from yeasts to humans. The m7GpppN cap promotes RNA stability, processing, transport and translation. Deletion of capping enzymes in yeasts was shown to be lethal due to rapid exonucleolytic degradation of uncapped transcripts or failure of capped but unmethylated RNA to initiate protein synthesis. Using RNAi and C. elegans, we have found that RNA capping is also essential for metazoan viability. C. elegans bifunctional capping enzyme was cloned, and capping activity by the expressed protein, as well as growth complementation of yeast deletion strains missing either RNA triphosphatase or guanylytransferase, required terminal sequences not present in the previously isolated cel-1 clone. By RNAi analysis we show that cel-1 is required for embryogenesis. cel-1 (RNAi) embryos formed cytoplasmic granules characteristic of a phenocluster of RNA processing genes and died early in development.
J. Biol. Chem, 10.1074/jbc.M212102200
Submitted on November 27, 2002
Revised on February 7, 2003
Accepted on February 7, 2003
mRNA capping requirement for C. elegans viability
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