JBC INTERFERin siRNA transfection reagent

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on April 25, 2003
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
278/18/15922    most recent
M212475200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Litvin, T. N.
Right arrow Articles by Levin, L. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Litvin, T. N.
Right arrow Articles by Levin, L. R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print February 27, 2003
J. Biol. Chem, 10.1074/jbc.M212475200
Submitted on December 9, 2002
Revised on February 13, 2003
Accepted on February 27, 2003

Kinetic properties of 'soluble' adenylyl cyclase: Synergism between calcium and bicarbonate

Tatiana N. Litvin, Margarita Kamenetsky, Alla Zarifyan, Jochen Buck, and Lonny R. Levin

Pharmacology, Weill Medical College of Cornell University, New York, NY 10021

Corresponding Author: jobuck{at}med.cornell.edu

‘Soluble’ adenylyl cyclase (sAC) is a widely expressed source of cAMP in mammalian cells which is evolutionarily, structurally, and biochemically distinct from the G protein responsive, transmembrane adenylyl cyclases (tmACs). In contrast to tmACs, sAC is insensitive to heterotrimeric G protein regulation and forskolin stimulation and is uniquely modulated by bicarbonate ions. Here we present the first report detailing kinetic analysis and biochemical properties of purified recombinant sAC. We confirm that bicarbonate regulation is conserved among mammalian sAC orthologs and demonstrate that bicarbonate stimulation is consistent with an increase in the enzyme’s Vmax with little effect on the apparent Km for substrate, ATP-Mg2+. Bicarbonate can further increase sAC activity by relieving substrate inhibition. We also identify calcium as a direct modulator of sAC activity. In contrast to bicarbonate, calcium stimulates sAC activity by decreasing its apparent Km for ATP-Mg2+. Due to their different mechanisms, calcium and bicarbonate synergistically activate sAC; therefore, small changes of either calcium or bicarbonate will lead to significant changes in cellular cAMP levels.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Gen. Physiol.Home page
L. S. Ramos, J. H. Zippin, M. Kamenetsky, J. Buck, and L. R. Levin
Glucose and GLP-1 Stimulate cAMP Production via Distinct Adenylyl Cyclases in INS-1E Insulinoma Cells
J. Gen. Physiol., August 25, 2008; 132(3): 329 - 338.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
E. Rodriguez-Miranda, M. G Buffone, S. E Edwards, T. S Ord, K. Lin, M. D Sammel, G. L Gerton, S. B Moss, and C. J Williams
Extracellular Adenosine 5'-Triphosphate Alters Motility and Improves the Fertilizing Capability of Mouse Sperm
Biol Reprod, July 1, 2008; 79(1): 164 - 171.
[Abstract] [Full Text] [PDF]

Home page
 Learn. Mem.Home page
A. R. Brennan, B. Dolinsky, M.-A. T. Vu, M. Stanley, M. F. Yeckel, and A. F.T. Arnsten
Blockade of IP3-mediated SK channel signaling in the rat medial prefrontal cortex improves spatial working memory
Learn. Mem., February 19, 2008; 15(3): 93 - 96.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
T. G. Paunescu, N. Da Silva, L. M. Russo, M. McKee, H. A. J. Lu, S. Breton, and D. Brown
Association of soluble adenylyl cyclase with the V-ATPase in renal epithelial cells
Am J Physiol Renal Physiol, January 1, 2008; 294(1): F130 - F138.
[Abstract] [Full Text] [PDF]

Home page
 Reproductive SciencesHome page
S. E. Edwards, M. G. Buffone, G. R. Knee, M. Rossato, G. Bonanni, S. Masiero, S. Ferasin, G. L. Gerton, S. B. Moss, and C. J. Williams
Effects of Extracellular Adenosine 5'-Triphosphate on Human Sperm Motility
Reproductive Sciences, October 1, 2007; 14(7): 655 - 666.
[Abstract] [PDF]

Home page
 J AndrolHome page
K. Schlingmann, M. A. Michaut, J. L. Mcelwee, C. A. Wolff, A. J. Travis, and R. M. Turner
Calmodulin and CaMKII in the Sperm Principal Piece: Evidence for a Motility-Related Calcium/Calmodulin Pathway
J Androl, September 1, 2007; 28(5): 706 - 716.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Physiol.Home page
A. Schmid, Z. Sutto, M.-C. Nlend, G. Horvath, N. Schmid, J. Buck, L. R. Levin, G. E. Conner, N. Fregien, and M. Salathe
Soluble Adenylyl Cyclase Is Localized to Cilia and Contributes to Ciliary Beat Frequency Regulation via Production of cAMP
J. Gen. Physiol., July 1, 2007; 130(1): 99 - 109.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. Wang, J. Hu, I. A. Bobulescu, T. A. Quill, P. McLeroy, O. W. Moe, and D. L. Garbers
A sperm-specific Na+/H+ exchanger (sNHE) is critical for expression and in vivo bicarbonate regulation of the soluble adenylyl cyclase (sAC)
PNAS, May 29, 2007; 104(22): 9325 - 9330.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
M. Kujala, S. Hihnala, J. Tienari, K. Kaunisto, J. Hastbacka, C. Holmberg, J. Kere, and P. Hoglund
Expression of ion transport-associated proteins in human efferent and epididymal ducts
Reproduction, April 1, 2007; 133(4): 775 - 784.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. M. Stessin, J. H. Zippin, M. Kamenetsky, K. C. Hess, J. Buck, and L. R. Levin
Soluble Adenylyl Cyclase Mediates Nerve Growth Factor-induced Activation of Rap1
J. Biol. Chem., June 23, 2006; 281(25): 17253 - 17258.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
A. Darszon, J. J Acevedo, B. E Galindo, E. O Hernandez-Gonzalez, T. Nishigaki, C. L Trevino, C. Wood, and C. Beltran
Sperm channel diversity and functional multiplicity.
Reproduction, June 1, 2006; 131(6): 977 - 988.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
L. R. Fraser, E. Beyret, S. R. Milligan, and S. A. Adeoya-Osiguwa
Effects of estrogenic xenobiotics on human and mouse spermatozoa
Hum. Reprod., May 1, 2006; 21(5): 1184 - 1193.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. T. Branham, L. S. Mayorga, and C. N. Tomes
Calcium-induced Acrosomal Exocytosis Requires cAMP Acting through a Protein Kinase A-independent, Epac-mediated Pathway
J. Biol. Chem., March 31, 2006; 281(13): 8656 - 8666.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Steegborn, T. N. Litvin, K. C. Hess, A. B. Capper, R. Taussig, J. Buck, L. R. Levin, and H. Wu
A Novel Mechanism for Adenylyl Cyclase Inhibition from the Crystal Structure of Its Complex with Catechol Estrogen
J. Biol. Chem., September 9, 2005; 280(36): 31754 - 31759.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
H. Han, A. Stessin, J. Roberts, K. Hess, N. Gautam, M. Kamenetsky, O. Lou, E. Hyde, N. Nathan, W. A. Muller, et al.
Calcium-sensing soluble adenylyl cyclase mediates TNF signal transduction in human neutrophils
J. Exp. Med., August 1, 2005; 202(3): 353 - 361.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
S. Masuda and T.-a. Ono
Adenylyl Cyclase Activity of Cya1 from the Cyanobacterium Synechocystis sp. Strain PCC 6803 Is Inhibited by Bicarbonate
J. Bacteriol., July 15, 2005; 187(14): 5032 - 5035.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
W. Geng, Z. Wang, J. Zhang, B. Y. Reed, C. Y. C. Pak, and O. W. Moe
Cloning and characterization of the human soluble adenylyl cyclase
Am J Physiol Cell Physiol, June 1, 2005; 288(6): C1305 - C1316.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Beaulieu, N. Da Silva, N. Pastor-Soler, C. R. Brown, P. J. S. Smith, D. Brown, and S. Breton
Modulation of the Actin Cytoskeleton via Gelsolin Regulates Vacuolar H+-ATPase Recycling
J. Biol. Chem., March 4, 2005; 280(9): 8452 - 8463.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. Luconi, I. Porazzi, P. Ferruzzi, S. Marchiani, G. Forti, and E. Baldi
Tyrosine Phosphorylation of the A Kinase Anchoring Protein 3 (AKAP3) and Soluble Adenylate Cyclase Are Involved in the Increase of Human Sperm Motility by Bicarbonate
Biol Reprod, January 1, 2005; 72(1): 22 - 32.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
Z. Sutto, G. E Conner, and M. Salathe
Regulation of human airway ciliary beat frequency by intracellular pH
J. Physiol., October 15, 2004; 560(2): 519 - 532.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
B. Marquez and S. S. Suarez
Different Signaling Pathways in Bovine Sperm Regulate Capacitationand Hyperactivation
Biol Reprod, June 1, 2004; 70(6): 1626 - 1633.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
R. A. Bundey and P. A. Insel
Discrete Intracellular Signaling Domains of Soluble Adenylyl Cyclase: Camps of cAMP?
Sci. Signal., May 4, 2004; 2004(231): pe19 - pe19.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
J. H. Zippin, J. Farrell, D. Huron, M. Kamenetsky, K. C. Hess, D. A. Fischman, L. R. Levin, and J. Buck
Bicarbonate-responsive "soluble" adenylyl cyclase defines a nuclear cAMP microdomain
J. Cell Biol., February 16, 2004; 164(4): 527 - 534.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
C. Rodeheffer and B. D. Shur
Sperm from {beta}1,4-galactosyltransferase I-null mice exhibit precocious capacitation
Development, February 1, 2004; 131(3): 491 - 501.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. S. Jaiswal and M. Conti
Calcium regulation of the soluble adenylyl cyclase expressed in mammalian spermatozoa
PNAS, September 16, 2003; 100(19): 10676 - 10681.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. J. Cann, A. Hammer, J. Zhou, and T. Kanacher
A Defined Subset of Adenylyl Cyclases Is Regulated by Bicarbonate Ion
J. Biol. Chem., September 12, 2003; 278(37): 35033 - 35038.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.