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M213043200v1
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Papers In Press, published online ahead of print May 19, 2003
J. Biol. Chem, 10.1074/jbc.M213043200
Submitted on December 20, 2002
Revised on May 19, 2003
Accepted on May 19, 2003

Adjacent sequence controls the response polarity of nitric oxide sensitive Sp factor binding sites

Jianhua Zhang, Shuibang Wang, Robert A. Wesley, and Robert L. Danner

Critical Care Medicine Department, National Institutes of Health, Bethesda, MD 20892

Corresponding Author: rdanner{at}nih.gov

Nitric oxide (NO• ) and cAMP-dependent protein kinase (PKA) inhibitors up-regulate tumor necrosis factor a (TNFalpha ) by decreasing Sp1 binding to a proximal GC box element. Here, elements flanking GC boxes were tested for their role in determining whether Sp sites act as activators or repressors. Promoter studies in receptive human cell-lines demonstrated that NO• down-regulated endothelial NO• synthase (eNOS) but up-regulated TNFalpha . Like TNFalpha , Sp1 binding to the eNOS promoter was decreased by NO•, a PKA inhibitor, H89, and increased by a PKA activator, dibutyryl cAMP (Bt2cAMP). For either promoter, mutation of Sp sites abolished NO• responses. In contrast, mutation of an upstream AP1 site in the TNFalpha promoter (not present in eNOS) maintained NO• responsiveness, but reversed the direction of NO• and cAMP effects. Using artificial constructs, NO• increased transcription when Sp and AP1 sites were both present (TNFalpha -like response), but decreased it when the adjacent AP1 site was disrupted (eNOS-like response). NO• , H89, and Bt2cAMP were found to produce reciprocal protein binding changes at contiguous AP1 and Sp sites (p < 0.0001 for an interaction). Chromatin immunoprecipitation assays demonstrated that Sp1 and to a lesser extent Sp3 bound to the GC-box regions of eNOS and TNFalpha in intact cells. Thus, this NO• - and cAMP-responsive regulatory module has a Sp site sensor variably coupled to an adjacent element that determines response polarity. These results define a composite element that can utilize secondary inputs to convert off signals to on, thereby conferring complex functionalities to the same DNA binding motif.


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