| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print January 2, 2003
Medicine, Division of Cardiology, Albert Einstein College of Medicine, Bronx, NY 10461
Corresponding Author: ypeng{at}aecom.yu.edu
Human Von Willebrand factor (VWF) gene sequences +155 to +247 contain cis-acting elements that contribute towards endothelial specific activation of the VWF promoter. Analyses of this region demonstrated the presence of a GATA binding site that is necessary for the promoter activation in endothelial cells. We have recently reported the presence of a novel NFY binding sequence in this region that does not conform to the consensus NFY binding sequence CCAAT. NFY was shown to function as a repressor of the VWF promoter through interaction with this novel binding site. Here we report that the NFY interacts with histone deacetylases (HDACs) in a cell-type specific manner and recruits them to the VWF promoter to inhibit the promoter activity in non-endothelial cells. Analyses of the acetylation status of histones in the chromatin region containing the VWF promoter sequences demonstrated that these sequences are associated with acetylated histone H4 specifically in endothelial cells. It was also demonstrated that HDACs are specifically recruited to the same chromatin region in non-endothelial cells. We also demonstrated that GATA6 is the GATA family member that interacts with the VWF promoter and that GATA6 is associated with NFY specifically in non-endothelial cells. We propose that NFY recruits HDACs to the VWF promoter, which may result in deacetylation of GATA6 as well as of histones in non-endothelial cells, thus leading to promoter inactivation. In endothelial cells however, association of HDACs, NFY and GATA6 is interrupted potentially through endothelial-cell specific signaling/mechanism, thus favoring the balance towards acetylation and activation of the VWF promoter.
J. Biol. Chem, 10.1074/jbc.M213156200
Submitted on December 23, 2002
Revised on January 2, 2003
Accepted on January 2, 2003
The NFY transcription factor inhibits VWF promoter activation in non-endothelial cells through recruitment of histone deacetylases
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Zhang, B. Chen, Y. Li, J. Chen, G. Lou, M. Chen, and D. Zhou Transcriptional Regulation of the Human PNRC Promoter by NFY in HepG2 Cells J. Biochem., May 1, 2008; 143(5): 675 - 683. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Matouk and P. A. Marsden Epigenetic Regulation of Vascular Endothelial Gene Expression Circ. Res., April 25, 2008; 102(8): 873 - 887. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Kleinschmidt, M. Nassiri, M. S. Stitt, K. Wasserloos, S. C. Watkins, B. R. Pitt, and N. Jahroudi Sequences in Intron 51 of the von Willebrand Factor Gene Target Promoter Activation to a Subset of Lung Endothelial Cells in Transgenic Mice J. Biol. Chem., February 1, 2008; 283(5): 2741 - 2750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. C. Aird Phenotypic Heterogeneity of the Endothelium: I. Structure, Function, and Mechanisms Circ. Res., February 2, 2007; 100(2): 158 - 173. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Hu, J.-F. Lu, R. Luo, S. Sen, and S. N. Maity Inhibition of CBF/NF-Y mediated transcription activation arrests cells at G2/M phase and suppresses expression of genes activated at G2/M phase of the cell cycle Nucleic Acids Res., December 4, 2006; 34(21): 6272 - 6285. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Takahashi, N. Hayashi, S. Kaminogawa, and C. Ra Molecular Mechanisms for Transcriptional Regulation of Human High-Affinity IgE Receptor beta-Chain Gene Induced by GM-CSF J. Immunol., October 1, 2006; 177(7): 4605 - 4611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Basile, R. Mantovani, and C. Imbriano DNA Damage Promotes Histone Deacetylase 4 Nuclear Localization and Repression of G2/M Promoters, via p53 C-terminal Lysines J. Biol. Chem., January 27, 2006; 281(4): 2347 - 2357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Fish, C. C. Matouk, A. Rachlis, S. Lin, S. C. Tai, C. D'Abreo, and P. A. Marsden The Expression of Endothelial Nitric-oxide Synthase Is Controlled by a Cell-specific Histone Code J. Biol. Chem., July 1, 2005; 280(26): 24824 - 24838. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Imbriano, A. Gurtner, F. Cocchiarella, S. Di Agostino, V. Basile, M. Gostissa, M. Dobbelstein, G. Del Sal, G. Piaggio, and R. Mantovani Direct p53 Transcriptional Repression: In Vivo Analysis of CCAAT-Containing G2/M Promoters Mol. Cell. Biol., May 1, 2005; 25(9): 3737 - 3751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Wei, W. K. Miskimins, and R. Miskimins Stage-specific Expression of Myelin Basic Protein in Oligodendrocytes Involves Nkx2.2-mediated Repression That Is Relieved by the Sp1 Transcription Factor J. Biol. Chem., April 22, 2005; 280(16): 16284 - 16294. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Hough, C. D. Cuthbert, C. Notley, C. Brown, C. Hegadorn, E. Berber, and D. Lillicrap Cell type-specific regulation of von Willebrand factor expression by the E4BP4 transcriptional repressor Blood, February 15, 2005; 105(4): 1531 - 1539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wang, Y. Peng, Y. Ma, and N. Jahroudi Histone H1-like protein participates in endothelial cell-specific activation of the von Willebrand factor promoter Blood, September 15, 2004; 104(6): 1725 - 1732. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Schuettengruber, E. Simboeck, H. Khier, and C. Seiser Autoregulation of Mouse Histone Deacetylase 1 Expression Mol. Cell. Biol., October 1, 2003; 23(19): 6993 - 7004. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Caretti, V. Salsi, C. Vecchi, C. Imbriano, and R. Mantovani Dynamic Recruitment of NF-Y and Histone Acetyltransferases on Cell-cycle Promoters J. Biol. Chem., August 15, 2003; 278(33): 30435 - 30440. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
