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Papers In Press, published online ahead of print April 3, 2003
Physiology Dept., National Cheng-Kung University, Medical College, Tainan 701
Corresponding Author: mjtang1{at}mail.ncku.edu.tw
Previous studies have shown that collagen gel overlay induced selective proteolysis of focal adhesion complex proteins in MDCK cells. In this study, we examined whether morphological and biochemical changes were present in cells cultured on collagen gel. We found that focal adhesion complex proteins, including FAK, talin, paxillin and p130cas, but not vinculin, were decreased within 1 h when MDCK cells were cultured on collagen gel. Collagen gel-induced selective decrease of focal adhesion proteins was observed in all lines of cells examined, including epithelial, fibroblastic and cancer cells. Matrigel also induced selective down-regulation of focal adhesion proteins. However, cells cultured on collagen gel- or matrigel-coated dishes did not show any changes of focal adhesion proteins. These data suggest that the physical nature of the gel, i.e. the rigidity, is involved in the expression of focal adhesion proteins. The collagen gel-induced down-regulation of focal adhesion complex proteins was caused by reduction of protein synthesis and activation of proteases such as calpain and was mediated by a2b1 integrin, but not DDR1. Furthermore, freshly isolated renal proximal tubule (PT) cells, initially exhibited little focal adhesion complex proteins, re-expressed focal adhesion complex proteins in primary cultures when they were cultured on collagen gel- or matrigel-coated dishes. Nevertheless, PT cells cultured on collagen gel or matrigel did not re-express focal adhesion complex proteins in primary culture. Taken together, these data indicate that the substratum rigidity controls expression of focal adhesion complex proteins, which may be of physiological significance.
J. Biol. Chem, 10.1074/jbc.M300092200
Submitted on January 6, 2003
Revised on April 3, 2003
Accepted on April 3, 2003
Rigidity of collagen fibrils controls collagen gel-induced down-regulation of focal adhesion complex proteins mediated by a2b1 integrin
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