JBC INTERFERin siRNA transfection reagent

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on July 3, 2003
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
278/28/26287    most recent
M303395200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hosokawa, N.
Right arrow Articles by Nagata, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hosokawa, N.
Right arrow Articles by Nagata, K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print May 6, 2003
J. Biol. Chem, 10.1074/jbc.M303395200
Submitted on April 2, 2003
Revised on May 3, 2003
Accepted on May 6, 2003

Enhancement of ER degradation of misfolded null Hong Kong alpha 1-antitrypsin by human ER mannosidase I

Nobuko Hosokawa, Linda O. Tremblay, Zhipeng You, Annette Herscovics, Ikuo Wada, and Kazuhiro Nagata

Department of Molecular and Cellualr Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8397

Corresponding Author: nobuko{at}frontier.kyoto-u.ac.jp

Misfolded glycoproteins synthesized in the endoplasmic reticulum (ER) are degraded by cytoplasmic proteasomes, a mechanism known as ERAD (ER-associated degradation). In the present study, we demonstrate that ERAD of the misfolded genetic variant null Hong Kong alpha 1-antitrypsin is enhanced by overexpression of the ER processing alpha 1,2-mannosidase (ER ManI) in HEK 293 cells, indicating the importance of ER ManI in glycoprotein quality control. We showed previously that EDEM, an enzymatically inactive mannosidase homolog, interacts with misfolded alpha 1-antitrypsin and accelerates its degradation (Hosokawa et al. (2001) EMBO Reports 2: 415-422). Herein we demonstrate a combined effect of ER ManI and EDEM on ERAD of misfolded alpha 1-antitrypsin. We also show that misfolded alpha 1-antitrypsin NHK contains labeled Glc1Man9GlcNAc and Man5-9GlcNAc released by endo-beta -N-acetylglucosaminidase H in pulse-chase experiments with [2-3H] mannose. Overexpression of ER ManI greatly increases the formation of Man8GlcNAc, induces the formation of Glc1Man8GlcNAc and increases trimming to Man5-7GlcNAc. We propose a model whereby the misfolded glycoprotein interacts with ER ManI and with EDEM, before being recognized by downstream ERAD components. This detailed characterization of oligosaccharides associated with a misfolded glycoprotein raises the possibility that the carbohydrate recognition determinant triggering ERAD may not be restricted to Man8GlcNAc2 isomer B as previous studies have suggested.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Hum Mol GenetHome page
M. Bartoli, E. Gicquel, L. Barrault, T. Soheili, M. Malissen, B. Malissen, N. Vincent-Lacaze, N. Perez, B. Udd, O. Danos, et al.
Mannosidase I inhibition rescues the human {alpha}-sarcoglycan R77C recurrent mutation
Hum. Mol. Genet., May 1, 2008; 17(9): 1214 - 1221.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. J. Caramelo and A. J. Parodi
Getting In and Out from Calnexin/Calreticulin Cycles
J. Biol. Chem., April 18, 2008; 283(16): 10221 - 10225.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
U. R. Mbonye, C. Yuan, C. E. Harris, R. S. Sidhu, I. Song, T. Arakawa, and W. L. Smith
Two Distinct Pathways for Cyclooxygenase-2 Protein Degradation
J. Biol. Chem., March 28, 2008; 283(13): 8611 - 8623.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
N. Kawasaki, Y. Ichikawa, I. Matsuo, K. Totani, N. Matsumoto, Y. Ito, and K. Yamamoto
The sugar-binding ability of ERGIC-53 is enhanced by its interaction with MCFD2
Blood, February 15, 2008; 111(4): 1972 - 1979.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
E. Avezov, Z. Frenkel, M. Ehrlich, A. Herscovics, and G. Z. Lederkremer
Endoplasmic Reticulum (ER) Mannosidase I Is Compartmentalized and Required for N-Glycan Trimming to Man5 6GlcNAc2 in Glycoprotein ER-associated Degradation
Mol. Biol. Cell, January 1, 2008; 19(1): 216 - 225.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
D. N. Hebert and M. Molinari
In and Out of the ER: Protein Folding, Quality Control, Degradation, and Related Human Diseases
Physiol Rev, October 1, 2007; 87(4): 1377 - 1408.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
K. Kato and Y. Kamiya
Structural views of glycoprotein-fate determination in cells
Glycobiology, October 1, 2007; 17(10): 1031 - 1044.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Banerjee, P. Vishwanath, J. Cui, D. J. Kelleher, R. Gilmore, P. W. Robbins, and J. Samuelson
The evolution of N-glycan-dependent endoplasmic reticulum quality control factors for glycoprotein folding and degradation
PNAS, July 10, 2007; 104(28): 11676 - 11681.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Zuber, J. H. Cormier, B. Guhl, R. Santimaria, D. N. Hebert, and J. Roth
EDEM1 reveals a quality control vesicular transport pathway out of the endoplasmic reticulum not involving the COPII exit sites
PNAS, March 13, 2007; 104(11): 4407 - 4412.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Wu, D. J. Termine, M. T. Swulius, K. W. Moremen, and R. N. Sifers
Human Endoplasmic Reticulum Mannosidase I Is Subject to Regulated Proteolysis
J. Biol. Chem., February 16, 2007; 282(7): 4841 - 4849.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
U. R. Mbonye, M. Wada, C. J. Rieke, H.-Y. Tang, D. L. DeWitt, and W. L. Smith
The 19-amino Acid Cassette of Cyclooxygenase-2 Mediates Entry of the Protein into the Endoplasmic Reticulum-associated Degradation System
J. Biol. Chem., November 24, 2006; 281(47): 35770 - 35778.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
L. W. Ruddock and M. Molinari
N-glycan processing in ER quality control
J. Cell Sci., November 1, 2006; 119(21): 4373 - 4380.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
C. Wojcik, M. Rowicka, A. Kudlicki, D. Nowis, E. McConnell, M. Kujawa, and G. N. DeMartino
Valosin-containing Protein (p97) Is a Regulator of Endoplasmic Reticulum Stress and of the Degradation of N-End Rule and Ubiquitin-Fusion Degradation Pathway Substrates in Mammalian Cells
Mol. Biol. Cell, November 1, 2006; 17(11): 4606 - 4618.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
B. Mueller, B. N. Lilley, and H. L. Ploegh
SEL1L, the homologue of yeast Hrd3p, is involved in protein dislocation from the mammalian ER
J. Cell Biol., October 23, 2006; 175(2): 261 - 270.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
S. J. Marciniak and D. Ron
Endoplasmic reticulum stress signaling in disease.
Physiol Rev, October 1, 2006; 86(4): 1133 - 1149.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
T. Gotoh and M. Mori
Nitric Oxide and Endoplasmic Reticulum Stress
Arterioscler. Thromb. Vasc. Biol., July 1, 2006; 26(7): 1439 - 1446.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
N. Hosokawa, I. Wada, Y. Natsuka, and K. Nagata
EDEM accelerates ERAD by preventing aberrant dimer formation of misfolded alpha1-antitrypsin.
Genes Cells, May 1, 2006; 11(5): 465 - 476.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Hirao, Y. Natsuka, T. Tamura, I. Wada, D. Morito, S. Natsuka, P. Romero, B. Sleno, L. O. Tremblay, A. Herscovics, et al.
EDEM3, a Soluble EDEM Homolog, Enhances Glycoprotein Endoplasmic Reticulum-associated Degradation and Mannose Trimming
J. Biol. Chem., April 7, 2006; 281(14): 9650 - 9658.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
B. Di Jeso, Y.-n. Park, L. Ulianich, A. S. Treglia, M. L. Urbanas, S. High, and P. Arvan
Mixed-Disulfide Folding Intermediates between Thyroglobulin and Endoplasmic Reticulum Resident Oxidoreductases ERp57 and Protein Disulfide Isomerase
Mol. Cell. Biol., November 15, 2005; 25(22): 9793 - 9805.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
F. Movsichoff, O. A. Castro, and A. J. Parodi
Characterization of Schizosaccharomyces pombe ER {alpha}-Mannosidase: A Reevaluation of the Role of the Enzyme on ER-associated Degradation
Mol. Biol. Cell, October 1, 2005; 16(10): 4714 - 4724.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Karaveg and K. W. Moremen
Energetics of Substrate Binding and Catalysis by Class 1 (Glycosylhydrolase Family 47) {alpha}-Mannosidases Involved in N-Glycan Processing and Endoplasmic Reticulum Quality Control
J. Biol. Chem., August 19, 2005; 280(33): 29837 - 29848.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Karaveg, A. Siriwardena, W. Tempel, Z.-J. Liu, J. Glushka, B.-C. Wang, and K. W. Moremen
Mechanism of Class 1 (Glycosylhydrolase Family 47) {alpha}-Mannosidases Involved in N-Glycan Processing and Endoplasmic Reticulum Quality Control
J. Biol. Chem., April 22, 2005; 280(16): 16197 - 16207.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
S. W. Mast, K. Diekman, K. Karaveg, A. Davis, R. N. Sifers, and K. W. Moremen
Human EDEM2, a novel homolog of family 47 glycosidases, is involved in ER-associated degradation of glycoproteins
Glycobiology, April 1, 2005; 15(4): 421 - 436.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Olivari, C. Galli, H. Alanen, L. Ruddock, and M. Molinari
A Novel Stress-induced EDEM Variant Regulating Endoplasmic Reticulum-associated Glycoprotein Degradation
J. Biol. Chem., January 28, 2005; 280(4): 2424 - 2428.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. K. Eriksson, R. Vago, V. Calanca, C. Galli, P. Paganetti, and M. Molinari
EDEM Contributes to Maintenance of Protein Folding Efficiency and Secretory Capacity
J. Biol. Chem., October 22, 2004; 279(43): 44600 - 44605.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Tempel, K. Karaveg, Z.-J. Liu, J. Rose, B.-C. Wang, and K. W. Moremen
Structure of Mouse Golgi {alpha}-Mannosidase IA Reveals the Molecular Basis for Substrate Specificity among Class 1 (Family 47 Glycosylhydrolase) {alpha}1,2-Mannosidases
J. Biol. Chem., July 9, 2004; 279(28): 29774 - 29786.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Svedine, T. Wang, R. Halaban, and D. N. Hebert
Carbohydrates act as sorting determinants in ER-associated degradation of tyrosinase
J. Cell Sci., June 15, 2004; 117(14): 2937 - 2949.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Wujek, E. Kida, M. Walus, K. E. Wisniewski, and A. A. Golabek
N-Glycosylation Is Crucial for Folding, Trafficking, and Stability of Human Tripeptidyl-peptidase I
J. Biol. Chem., March 26, 2004; 279(13): 12827 - 12839.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Horibe, M. Gomi, D. Iguchi, H. Ito, Y. Kitamura, T. Masuoka, I. Tsujimoto, T. Kimura, and M. Kikuchi
Different Contributions of the Three CXXC Motifs of Human Protein-disulfide Isomerase-related Protein to Isomerase Activity and Oxidative Refolding
J. Biol. Chem., February 6, 2004; 279(6): 4604 - 4611.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z. Frenkel, W. Gregory, S. Kornfeld, and G. Z. Lederkremer
Endoplasmic Reticulum-associated Degradation of Mammalian Glycoproteins Involves Sugar Chain Trimming to Man6-5GlcNAc2
J. Biol. Chem., September 5, 2003; 278(36): 34119 - 34124.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.