| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print August 22, 2003
Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455
Corresponding Author: bernl001{at}umn.edu
Fatty acid transport protein 1 (FATP1) is an ~63 kDa plasma membrane protein that facilitates the influx of fatty acids into adipocytes as well as skeletal and cardiac myocytes. Previous studies with FATP1 expressed in COS1 cell extracts suggested that FATP1 exhibits very long chain acyl CoA synthetase activity and that such activity may be linked to fatty acid transport. To address the enzymatic activity of the isolated protein, murine FATP1 and acyl CoA synthetase 1 (ACS1) were engineered to contain a C-terminus myc-his tag, expressed in COS1 cells via adenoviral-mediated infection and purified to homogeneity using nickel affinity chromatography. Kinetic analysis of the purified enzymes was carried out for long chain palmitic acid (C16:0) and very long chain lignoceric acid (C24:0) as well as for ATP, and CoA. FATP1 exhibited similar substrate specificity for fatty acids 16 to 24 carbons in length while ACS1 was 10-fold more active on long chain fatty acids relative to very long chain fatty acids. The very long chain acyl CoA synthetase activity of the two enzymes was comparable as were the Km values for both ATP and Coenzyme A. Interestingly, FATP1 was insensitive to inhibition by triacsin C whereas ACS1 was inhibited by micromolar concentrations of the compound. These data represent the first characterization of purified FATP1 and indicate that the enzyme is a broad substrate specificity acyl CoA synthetase. These findings are consistent with the hypothesis that that fatty acid uptake into cells is linked to their esterification with Coenzyme A.
J. Biol. Chem, 10.1074/jbc.M306575200
Submitted on June 20, 2003
Revised on August 22, 2003
Accepted on August 22, 2003
Characterization of the Acyl CoA synthetase activity of purified murine fatty acid transport protein 1
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  R. W. Schwenk, J. J.F.P. Luiken, A. Bonen, and J. F.C. Glatz Regulation of sarcolemmal glucose and fatty acid transporters in cardiac disease Cardiovasc Res, July 15, 2008; 79(2): 249 - 258. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Watkins Very-long-chain Acyl-CoA Synthetases J. Biol. Chem., January 25, 2008; 283(4): 1773 - 1777. [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Watkins, D. Maiguel, Z. Jia, and J. Pevsner Evidence for 26 distinct acyl-coenzyme A synthetase genes in the human genome J. Lipid Res., December 1, 2007; 48(12): 2736 - 2750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Obermeyer, P. Fraisl, C. C. DiRusso, and P. N. Black Topology of the yeast fatty acid transport protein Fat1p: mechanistic implications for functional domains on the cytosolic surface of the plasma membrane J. Lipid Res., November 1, 2007; 48(11): 2354 - 2364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Moulson, M.-H. Lin, J. M. White, E. P. Newberry, N. O. Davidson, and J. H. Miner Keratinocyte-specific Expression of Fatty Acid Transport Protein 4 Rescues the Wrinkle-free Phenotype in Slc27a4/Fatp4 Mutant Mice J. Biol. Chem., May 25, 2007; 282(21): 15912 - 15920. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Lobo, B. M. Wiczer, A. J. Smith, A. M. Hall, and D. A. Bernlohr Fatty acid metabolism in adipocytes: functional analysis of fatty acid transport proteins 1 and 4 J. Lipid Res., March 1, 2007; 48(3): 609 - 620. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bonen, A. Chabowski, J. J. F. P Luiken, and J. F. C. Glatz Mechanisms and Regulation of Protein-Mediated Cellular Fatty Acid Uptake: Molecular, Biochemical, and Physiological Evidence Physiology, February 1, 2007; 22(1): 15 - 28. [Full Text] [PDF]
|
|
|
|
 |
|
 K. Milger, T. Herrmann, C. Becker, D. Gotthardt, J. Zickwolf, R. Ehehalt, P. A. Watkins, W. Stremmel, and J. Fullekrug Cellular uptake of fatty acids driven by the ER-localized acyl-CoA synthetase FATP4 J. Cell Sci., November 15, 2006; 119(22): 4678 - 4688. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Durgan, J. K. Smith, M. A. Hotze, O. Egbejimi, K. D. Cuthbert, V. G. Zaha, J. R. B. Dyck, E. D. Abel, and M. E. Young Distinct transcriptional regulation of long-chain acyl-CoA synthetase isoforms and cytosolic thioesterase 1 in the rodent heart by fatty acids and insulin Am J Physiol Heart Circ Physiol, June 1, 2006; 290(6): H2480 - H2497. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Pei, Z. Jia, and P. A. Watkins The Second Member of the Human and Murine "Bubblegum" Family Is a Testis- and Brainstem-specific Acyl-CoA Synthetase J. Biol. Chem., March 10, 2006; 281(10): 6632 - 6641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Richards, J. D. Harp, D. S. Ory, and J. E. Schaffer Fatty acid transport protein 1 and long-chain acyl coenzyme A synthetase 1 interact in adipocytes J. Lipid Res., March 1, 2006; 47(3): 665 - 672. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Kiens Skeletal Muscle Lipid Metabolism in Exercise and Insulin Resistance Physiol Rev, January 1, 2006; 86(1): 205 - 243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. M. Coort, W. A. Coumans, A. Bonen, G. J. van der Vusse, J. F. C. Glatz, and J. J. F. P. Luiken Divergent effects of rosiglitazone on protein-mediated fatty acid uptake in adipose and in muscle tissues of Zucker rats J. Lipid Res., June 1, 2005; 46(6): 1295 - 1302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. DiRusso, H. Li, D. Darwis, P. A. Watkins, J. Berger, and P. N. Black Comparative Biochemical Studies of the Murine Fatty Acid Transport Proteins (FATP) Expressed in Yeast J. Biol. Chem., April 29, 2005; 280(17): 16829 - 16837. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Hall, B. M. Wiczer, T. Herrmann, W. Stremmel, and D. A. Bernlohr Enzymatic Properties of Purified Murine Fatty Acid Transport Protein 4 and Analysis of Acyl-CoA Synthetase Activities in Tissues from FATP4 Null Mice J. Biol. Chem., March 25, 2005; 280(12): 11948 - 11954. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. Mashek, K. E. Bornfeldt, R. A. Coleman, J. Berger, D. A. Bernlohr, P. Black, C. C. DiRusso, S. A. Farber, W. Guo, N. Hashimoto, et al. Revised nomenclature for the mammalian long-chain acyl-CoA synthetase gene family J. Lipid Res., October 1, 2004; 45(10): 1958 - 1961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Weigert, K. Brodbeck, H. Staiger, C. Kausch, F. Machicao, H. U. Haring, and E. D. Schleicher Palmitate, but Not Unsaturated Fatty Acids, Induces the Expression of Interleukin-6 in Human Myotubes through Proteasome-dependent Activation of Nuclear Factor-{kappa}B J. Biol. Chem., June 4, 2004; 279(23): 23942 - 23952. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Schmelter, B. L. Trigatti, G. E. Gerber, and D. Mangroo Biochemical Demonstration of the Involvement of Fatty Acyl-CoA Synthetase in Fatty Acid Translocation across the Plasma Membrane J. Biol. Chem., June 4, 2004; 279(23): 24163 - 24170. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
