JBC Anatrace, Inc.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on December 5, 2003
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
278/49/49652    most recent
M308577200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rychlik, J. L.
Right arrow Articles by Lewis, E. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rychlik, J. L.
Right arrow Articles by Lewis, E. J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print September 23, 2003
J. Biol. Chem, 10.1074/jbc.M308577200
Submitted on August 5, 2003
Revised on September 18, 2003
Accepted on September 19, 2003

The interaction between dHAND and Arix at the dopamine-beta -hydroxylase promoter region is independent of direct dHAND binding to DNA

Jennifer L. Rychlik, Vincent Gerbasi, and Elaine J. Lewis

Biochemistry and Molecular Biology, Oregon Health and Sciences University, Portland, OR 97239

Corresponding Author: rychlikj{at}ohsu.edu

Dopamine-beta -hydroxylase (DBH) catalyzes the production of norepinephrine and its expression defines the noradrenergic phenotype. Transcription factors dHAND, a bHLH protein, and Arix/Phox2a, a homeoprotein, have been demonstrated to play a role in the differentiation and maintenance of catecholaminergic neurons. Three Arix regulatory sites have been identified in the DBH promoter proximal region, but there is no such evidence for dHAND. Co-transfection with a DBH promoter-luciferase reporter construct plus dHAND or dHAND/E12 expression plasmids did not alter luciferase activity, while transfection with Arix resulted in a 2.5-fold stimulation of luciferase activity. However, a 5.5-fold increase was observed if Arix and dHAND were combined and an 8-fold level of expression was observed if Arix was transfected with a dHAND mutant lacking the basic, DNA-binding domain. If the homeodomain sites in the DBH promoter proximal region were mutated, all activity was lost, demonstrating dependence upon Arix-DNA interaction for transcriptional activation. In electrophoretic mobility shift assays, the addition of dHAND decreased the amount of Arix needed to elicit a mobility shift with the DBH homeodomain binding sites, and the dHAND basic mutant potentiated Arix binding in a similar manner to wild-type dHAND. The dHAND/Arix complex was dissociated by the addition of a cold competitor containing a homeodomain, but not by the addition of a competitor containing E-boxes. Arix coprecipitated with antisera directed against recombinant dHAND, demonstrating direct protein-protein interactions. These results indicate that the activation of the DBH promoter by Arix is potentiated by dHAND via a mechanism independent of a direct interaction of dHAND with DNA.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Hum Mol GenetHome page
G. Wen, J. Wessel, W. Zhou, G. B. Ehret, F. Rao, M. Stridsberg, S. K. Mahata, P. M. Gent, M. Das, R. S. Cooper, et al.
An ancestral variant of Secretogranin II confers regulation by PHOX2 transcription factors and association with hypertension
Hum. Mol. Genet., July 15, 2007; 16(14): 1752 - 1764.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
V. R. Gerbasi and A. J. Link
The Myotonic Dystrophy Type 2 Protein ZNF9 Is Part of an ITAF Complex That Promotes Cap-independent Translation
Mol. Cell. Proteomics, June 1, 2007; 6(6): 1049 - 1058.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Benfante, A. Flora, S. Di Lascio, F. Cargnin, R. Longhi, S. Colombo, F. Clementi, and D. Fornasari
Transcription Factor PHOX2A Regulates the Human {alpha}3 Nicotinic Receptor Subunit Gene Promoter
J. Biol. Chem., May 4, 2007; 282(18): 13290 - 13302.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
M. E. Lucas, F. Muller, R. Rudiger, P. D. Henion, and H. Rohrer
The bHLH transcription factor hand2 is essential for noradrenergic differentiation of sympathetic neurons
Development, October 15, 2006; 133(20): 4015 - 4024.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
S. Fuke, N. Sasagawa, and S. Ishiura
Identification and Characterization of the Hesr1/Hey1 as a Candidate trans-Acting Factor on Gene Expression through the 3' Non-Coding Polymorphic Region of the Human Dopamine Transporter (DAT1) Gene
J. Biochem., February 1, 2005; 137(2): 205 - 216.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
K. Tsarovina, A. Pattyn, J. Stubbusch, F. Muller, J. van der Wees, C. Schneider, J.-F. Brunet, and H. Rohrer
Essential role of Gata transcription factors in sympathetic neuron development
Development, October 1, 2004; 131(19): 4775 - 4786.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.