| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print October 30, 2003
Glycobiology Program, The Burnham Institute, La Jolla, CA 92037
Corresponding Author: minoru{at}burnham.org
L-selectin mediates lymphocyte homing by facilitating lymphocyte adhesion to carbohydrate ligands expressed on high endothelial venules (HEV) of the secondary lymphoid organs. Previous studies demonstrated that L-selectin ligand sulfotransferase (LSST) forms 6-sulfo sialyl Lewis x (sLex) on both core 2 branch and MECA-79 positive extended core 1 O-glycans, but the chemical nature and roles of HEV ligands elaborated by LSST and core2 ß1,6-N-acetyglucosaminyltransferase-1 (Core2GlcNAcT) have been undefined. In the present study, we have generated mutant mice with deficient LSST and show that inactivation of LSST gene alone leads to only partial impairment of lymphocyte homing to peripheral lymph nodes, and moderate reduction in lymphocyte counts in the peripheral lymph nodes, despite the fact that L-selectin ligands that contain 6-sulfo sLex are reduced at HEV. By contrast, LSST/Core2GlcNAcT double null mice exhibited a markedly reduced lymphocyte homing and reduced lymphocyte counts as a result of significantly decreased 6-sulfo sLex on HEV L-selectin counterreceptors, relative to LSST- or Core2GlcNAcT- single null mice. Moreover, induction of LSST and Core2GlcNAcT transcripts was observed in HEV-like structure formed in the salivary gland of non-obese diabetic mouse, which displays chronic inflammation. These results indicate that LSST and Core2GlcNAcT cooperatively synthesize HEV-specific L-selectin ligands required for lymphocyte homing and suggest that LSST and Core2GlcNAcT play a critical role in lymphocyte trafficking during chronic inflammation.
J. Biol. Chem, 10.1074/jbc.M311150200
Submitted on October 10, 2003
Revised on October 28, 2003
Accepted on October 30, 2003
Core 2 branching ß1,6-N-acetylglucosaminyl transferase and high endothelial venule-restricted sulfotransferase collaboratively control lymphocyte homing
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Tenno, K. Ohtsubo, F. K. Hagen, D. Ditto, A. Zarbock, P. Schaerli, U. H. von Andrian, K. Ley, D. Le, L. A. Tabak, et al. Initiation of Protein O Glycosylation by the Polypeptide GalNAcT-1 in Vascular Biology and Humoral Immunity Mol. Cell. Biol., December 15, 2007; 27(24): 8783 - 8796. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Liao, K. Bentley, M. Lebrun, W. Lesslauer, F. H. Ruddle, and N. H. Ruddle Transgenic LacZ under control of Hec-6st regulatory sequences recapitulates endogenous gene expression on high endothelial venules PNAS, March 13, 2007; 104(11): 4577 - 4582. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Tjew, K. L. Brown, R. Kannagi, and P. Johnson Expression of N-acetylglucosamine 6-O-sulfotransferases (GlcNAc6STs)-1 and -4 in human monocytes: GlcNAc6ST-1 is implicated in the generation of the 6-sulfo N-acetyllactosamine/Lewis x epitope on CD44 and is induced by TNF-{alpha} Glycobiology, July 1, 2005; 15(7): 7C - 13C. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Toppila-Salmi, J. P. Myller, T. V. M. Torkkeli, J. V. Muhonen, J. A. Renkonen, M. E. Rautiainen, and R. L. O. Renkonen Endothelial L-Selectin Ligands in Sinus Mucosa during Chronic Maxillary Rhinosinusitis Am. J. Respir. Crit. Care Med., June 15, 2005; 171(12): 1350 - 1357. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D. Rosen, D. Tsay, M. S. Singer, S. Hemmerich, and W. M. Abraham Therapeutic Targeting of Endothelial Ligands for L-selectin (PNAd) in a Sheep Model of Asthma Am. J. Pathol., March 1, 2005; 166(3): 935 - 944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kobayashi, J. Mitoma, N. Nakamura, T. Katsuyama, J. Nakayama, and M. Fukuda Induction of peripheral lymph node addressin in human gastric mucosa infected by Helicobacter pylori PNAS, December 21, 2004; 101(51): 17807 - 17812. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-M. Gauguet, S. D. Rosen, J. D. Marth, and U. H. von Andrian Core 2 branching {beta}1,6-N-acetylglucosaminyltransferase and high endothelial cell N-acetylglucosamine-6-sulfotransferase exert differential control over B- and T-lymphocyte homing to peripheral lymph nodes Blood, December 15, 2004; 104(13): 4104 - 4112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. de Graffenried and C. R. Bertozzi The Stem Region of the Sulfotransferase GlcNAc6ST-1 Is a Determinant of Substrate Specificity J. Biol. Chem., September 17, 2004; 279(38): 40035 - 40043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Uchimura, K. Kadomatsu, F. M. El-Fasakhany, M. S. Singer, M. Izawa, R. Kannagi, N. Takeda, S. D. Rosen, and T. Muramatsu N-Acetylglucosamine 6-O-Sulfotransferase-1 Regulates Expression of L-Selectin Ligands and Lymphocyte Homing J. Biol. Chem., August 13, 2004; 279(33): 35001 - 35008. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
