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M402230200v1
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Papers In Press, published online ahead of print May 20, 2004
J. Biol. Chem, 10.1074/jbc.M402230200
Submitted on February 27, 2004
Revised on May 13, 2004
Accepted on May 20, 2004

AcrA, AcrB and TolC of Escherichia coli form a stable intermembrane multidrug efflux complex

Elena B. Tikhonova and Helen I. Zgurskaya

Chemistry and Biochemistry, University of Oklahoma, Norman, OK 73019

Corresponding Author: elenaz{at}ou.edu

Many transporters of gram-negative bacteria involved in the extracellular secretion of proteins and efflux of toxic molecules operate by forming intermembrane complexes. These complexes are proposed to span both, the inner and the outer membranes and create a bridge across the periplasm. In this study, we analyzed interactions between the inner and outer membrane components of the tri-partite multidrug efflux pump AcrAB-TolC from Escherichia coli. We found that once assembled, the intermembrane AcrAB-TolC complex is stable during the separation of the inner and outer membranes, and subsequent purification. All three components of the complex co-purify when the affinity tag is attached to either of the proteins suggesting bi-partite interactions between AcrA, AcrB, and TolC. We show that antibiotics, the substrates of AcrAB-TolC stabilize interactions within the complex. However, the formation of AcrAB-TolC complex does not require an input of energy.


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