JBC Avanti Polar Lipids

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on September 10, 2004
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
279/37/38160    most recent
M402885200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Canning, M.
Right arrow Articles by Everett, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Canning, M.
Right arrow Articles by Everett, R. D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print July 6, 2004
J. Biol. Chem, 10.1074/jbc.M402885200
Submitted on March 15, 2004
Revised on July 6, 2004
Accepted on July 6, 2004

A RING finger ubiquitin ligase is protected from auto-catalyzed ubiquitination and degradation by binding to ubiquitin-specific protease USP7

Mary Canning, Chris Boutell, Jane Parkinson, and Roger D. Everett

MRC Virology Unit, Glasgow, Scotland G11 5JR

Corresponding Author: r.everett{at}vir.gla.ac.uk

Herpes simplex virus type 1 Immediate-Early regulatory protein ICP0 stimulates lytic infection and reactivation from latency, processes that require the ubiquitin E3 ligase activity mediated by the RING-finger domain in the N-terminal portion of the protein. ICP0 stimulates the production of polyubiquitin chains by the ubiquitin conjugating enzymes UbcH5a and UbcH6 in vitro, and in infected and transfected cells it induces the proteasome-dependent degradation of a number of cellular proteins including PML, the major constituent protein of PML nuclear bodies. However, ICP0 binds strongly to the cellular ubiquitin-specific protease USP7, a member of a family of proteins that cleave polyubiquitin chains and/or ubiquitin precursors. The region of ICP0 that is required for its interaction with USP7 has been mapped and mutations in this domain reduce the functionality of ICP0. These findings pose the question of why ICP0 includes domains that are associated with the potentially antagonistic functions of ubiquitin conjugation and deconjugation. Here we report that although neither protein affected the intrinsic activities of the other in vitro, USP7 protected ICP0 from auto-ubiquitination in vitro and their interaction can greatly increase the stability of ICP0 in vivo. These results demonstrate that RING-finger mediated auto-ubiquitination of ICP0 is physiologically relevant and can be regulated by interaction with USP7. This principle may extend to a number of cellular RING finger E3 ubiquitin ligase proteins that have analogous interactions with ubiquitin-specific cleavage enzymes.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 GeneticsHome page
C. E. Rocheleau, K. Cullison, K. Huang, Y. Bernstein, A. C. Spilker, and M. V. Sundaram
The Caenorhabditis elegans ekl (Enhancer of ksr-1 Lethality) Genes Include Putative Components of a Germline Small RNA Pathway
Genetics, March 1, 2008; 178(3): 1431 - 1443.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Lerner, M. Corcoran, D. Cepeda, M. L. Nielsen, R. Zubarev, F. Ponten, M. Uhlen, S. Hober, D. Grander, and O. Sangfelt
The RBCC Gene RFP2 (Leu5) Encodes a Novel Transmembrane E3 Ubiquitin Ligase Involved in ERAD
Mol. Biol. Cell, May 1, 2007; 18(5): 1670 - 1682.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
J. W. Graff, J. Ewen, K. Ettayebi, and M. E. Hardy
Zinc-binding domain of rotavirus NSP1 is required for proteasome-dependent degradation of IRF3 and autoregulatory NSP1 stability
J. Gen. Virol., February 1, 2007; 88(2): 613 - 620.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Kee, W. Munoz, N. Lyon, and J. M. Huibregtse
The Deubiquitinating Enzyme Ubp2 Modulates Rsp5-dependent Lys63-linked Polyubiquitin Conjugates in Saccharomyces cerevisiae
J. Biol. Chem., December 1, 2006; 281(48): 36724 - 36731.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. D. Everett, S. Rechter, P. Papior, N. Tavalai, T. Stamminger, and A. Orr
PML Contributes to a Cellular Mechanism of Repression of Herpes Simplex Virus Type 1 Infection That Is Inactivated by ICP0.
J. Virol., August 1, 2006; 80(16): 7995 - 8005.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M.-C. Geoffroy, G. Chadeuf, A. Orr, A. Salvetti, and R. D. Everett
Impact of the Interaction between Herpes Simplex Virus Type 1 Regulatory Protein ICP0 and Ubiquitin-Specific Protease USP7 on Activation of Adeno-Associated Virus Type 2 rep Gene Expression.
J. Virol., April 1, 2006; 80(7): 3650 - 3654.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
C. Boutell, M. Canning, A. Orr, and R. D. Everett
Reciprocal Activities between Herpes Simplex Virus Type 1 Regulatory Protein ICP0, a Ubiquitin E3 Ligase, and Ubiquitin-Specific Protease USP7
J. Virol., October 1, 2005; 79(19): 12342 - 12354.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2004 by the American Society for Biochemistry and Molecular Biology.