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A more recent version of this article appeared on November 26, 2004
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M406773200v1
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Papers In Press, published online ahead of print September 21, 2004
J. Biol. Chem, 10.1074/jbc.M406773200
Submitted on June 17, 2004
Revised on September 21, 2004
Accepted on September 20, 2004

Crystal structure of PriB-a primosomal DNA replication protein of Escherichia coli

Jyung-Hurng Liu, Tsai-Wang Chang, Cheng-Yang Huang, Sue-Une Chen, Huey-Nan Wu, Ming-Chung Chang, and Chwan-Deng Hsiao

Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan 115

Corresponding Author: mbhsiao{at}ccvax.sinica.edu.tw

PriB is one of the Escherichia coli -type primosome proteins which are required for assembly of the primosome, a mobile multi-enzyme complex responsible for the initiation of DNA replication. Here we report the crystal structure of the Escherichia coli PriB at 2.1 Å resolution by multi-wavelength anomalous diffraction using a mercury derivative. The polypeptide chain of PriB is structurally similar to that of single-stranded DNA-binding protein (SSB). However, the biological unit of PriB is a dimer, not a homo-tetramer like SSB. Electrophoretic mobility shift assays demonstrated that PriB binds single-stranded DNA and single-stranded RNA with comparable affinity. We also show that PriB binds single-stranded DNA with certain base preferences. Base on the PriB structural information and biochemical studies, we propose that the potential tetramer formation surface and several other regions of PriB may participate in protein-protein interaction during DNA replication. These findings may illuminate the role of PriB in -type primosome assembly.


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 Nucleic Acids ResHome page
C.-Y. Huang, C.-H. Hsu, Y.-J. Sun, H.-N. Wu, and C.-D. Hsiao
Complexed crystal structure of replication restart primosome protein PriB reveals a novel single-stranded DNA-binding mode
Nucleic Acids Res., September 1, 2006; 34(14): 3878 - 3886.
[Abstract] [Full Text] [PDF]

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 J. Biol. Chem.Home page
C. J. Cadman, M. Lopper, P. B. Moon, J. L. Keck, and P. McGlynn
PriB Stimulates PriA Helicase via an Interaction with Single-stranded DNA
J. Biol. Chem., December 2, 2005; 280(48): 39693 - 39700.
[Abstract] [Full Text] [PDF]


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