JBC PeproTech; Our Business is Cytokines!

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on January 14, 2005
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
280/2/1448    most recent
M410104200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yamamoto, K.
Right arrow Articles by Ishihama, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yamamoto, K.
Right arrow Articles by Ishihama, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print November 2, 2004
J. Biol. Chem, 10.1074/jbc.M410104200
Submitted on September 2, 2004
Revised on November 1, 2004
Accepted on November 2, 2004

Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli

Kaneyoshi Yamamoto, Kiyo Hirao, Taku Oshima, Hirofumi Aiba, Ryutaro Utsumi, and Akira Ishihama

Department of Agricultural Chemistry, Kinki University, Nara, Nara 631-8505

Corresponding Author: kyamam{at}nara.kindai.ac.jp

Bacteria possess a signal transduction system, referred to as two component system, for adaptation to external stimuli. Each two component system (TCS) consists of a sensor protein-histidine kinase (HK) and a response regulator (RR), both together forming a signal transduction pathway via histidyl-aspartyl phosphorelay. A total of 30 sensor HKs including as yet uncharacterized putative HKs (BaeS, BasS, CreC, CusS, HydH, RstB, YedV and YfhK) and a total of 34 RRs including putative RRs (BaeR, BasR, CreB, CusR, HydG, RstA, YedW, YfhA, YgeK, and YhjB) have been suggested to exist in Escherichia coli. We have purified the carboxyl-terminal catalytic domain of 27 sensor HKs and the full-length protein of all 34 RRs to apparent homogeneity. Self-phosphorylation in vitro was detected for 25 HKs. The rate of self-phosphoorylation differed among HKs while the level of phosphorylation was generally co-related with the phosphorylation rate. However, the phosphorylation level was low for ArcB, HydH, NarQ and NtrB even though the reaction rate was fast, while the level was high for the slow-phosphorylation species, BasS, CheA and CreC. Using the phosphorylated HKs, we examined trans-phosphorylation in vitro of RRs for all possible combinations. Trans-phosphorylation of presumed cognate RRs by HKs was detected, for the first time, for eight pairs, BaeS-BaeR, BasS-BasR, CreC-CreB, CusS-CusR, HydH-HydG, RstB-RstA, YedV-YedW, and YfhK-YfhA. All trans-phosphorylation took place within less than 1/2 min, but the stability of phosphorylated RRs differed, indicating the involvement of de-phosphorylation control. In addition to the trans-phosphorylation between the cognate pairs, we detected trans-phosphorylation between about 3% of non-cognate HK-RR paris, raising a possibility that the cross-talk in signal transduction takes place between TCS systems.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
Y. H. Lee, J. H. Kim, I. S. Bang, and Y. K. Park
The Membrane-Bound Transcriptional Regulator CadC Is Activated by Proteolytic Cleavage in Response to Acid Stress
J. Bacteriol., July 15, 2008; 190(14): 5120 - 5126.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
P. Chong, L. Drake, and I. Biswas
LiaS Regulates Virulence Factor Expression in Streptococcus mutans
Infect. Immun., July 1, 2008; 76(7): 3093 - 3099.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Belcheva and D. Golemi-Kotra
A Close-up View of the VraSR Two-component System: A MEDIATOR OF STAPHYLOCOCCUS AUREUS RESPONSE TO CELL WALL DAMAGE
J. Biol. Chem., May 2, 2008; 283(18): 12354 - 12364.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-L. Hsu, H.-C. Chen, H.-L. Peng, and H.-Y. Chang
Characterization of the Histidine-containing Phosphotransfer Protein B-mediated Multistep Phosphorelay System in Pseudomonas aeruginosa PAO1
J. Biol. Chem., April 11, 2008; 283(15): 9933 - 9944.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
A. J. Wolfe, N. Parikh, B. P. Lima, and B. Zemaitaitis
Signal Integration by the Two-Component Signal Transduction Response Regulator CpxR
J. Bacteriol., April 1, 2008; 190(7): 2314 - 2322.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
K. Hamann, P. Zimmann, and K. Altendorf
Reduction of Turgor Is Not the Stimulus for the Sensor Kinase KdpD of Escherichia coli
J. Bacteriol., April 1, 2008; 190(7): 2360 - 2367.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
J. D. Partridge, D. F. Browning, M. Xu, L. J. Newnham, C. Scott, R. E. Roberts, R. K. Poole, and J. Green
Characterization of the Escherichia coli K-12 ydhYVWXUT operon: regulation by FNR, NarL and NarP
Microbiology, February 1, 2008; 154(2): 608 - 618.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
Y.-L. Tzeng, C. M. Kahler, X. Zhang, and D. S. Stephens
MisR/MisS Two-Component Regulon in Neisseria meningitidis
Infect. Immun., February 1, 2008; 76(2): 704 - 716.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Eguchi, J. Itou, M. Yamane, R. Demizu, F. Yamato, A. Okada, H. Mori, A. Kato, and R. Utsumi
B1500, a small membrane protein, connects the two-component systems EvgS/EvgA and PhoQ/PhoP in Escherichia coli
PNAS, November 20, 2007; 104(47): 18712 - 18717.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. L. Cabeza, A. Aguirre, F. C. Soncini, and E. G. Vescovi
Induction of RpoS Degradation by the Two-Component System Regulator RstA in Salmonella enterica
J. Bacteriol., October 15, 2007; 189(20): 7335 - 7342.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. K. Matta, E. E. Lioliou, C. H. Panagiotidis, D. A. Kyriakidis, and C. A. Panagiotidis
Interactions of the Antizyme AtoC with Regulatory Elements of the Escherichia coli atoDAEB Operon
J. Bacteriol., September 1, 2007; 189(17): 6324 - 6332.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
E. A. Hussa, T. M. O'Shea, C. L. Darnell, E. G. Ruby, and K. L. Visick
Two-Component Response Regulators of Vibrio fischeri: Identification, Mutagenesis, and Characterization
J. Bacteriol., August 15, 2007; 189(16): 5825 - 5838.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
K. E. Carlsson, J. Liu, P. J. Edqvist, and M. S. Francis
Extracytoplasmic-Stress-Responsive Pathways Modulate Type III Secretion in Yersinia pseudotuberculosis
Infect. Immun., August 1, 2007; 75(8): 3913 - 3924.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
H. Ogasawara, A. Hasegawa, E. Kanda, T. Miki, K. Yamamoto, and A. Ishihama
Genomic SELEX Search for Target Promoters under the Control of the PhoQP-RstBA Signal Relay Cascade
J. Bacteriol., July 1, 2007; 189(13): 4791 - 4799.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
N. Moker, J. Kramer, G. Unden, R. Kramer, and S. Morbach
In Vitro Analysis of the Two-Component System MtrB-MtrA from Corynebacterium glutamicum
J. Bacteriol., May 1, 2007; 189(9): 3645 - 3649.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
L. A. Bibb, C. A. Kunkle, and M. P. Schmitt
The ChrA-ChrS and HrrA-HrrS Signal Transduction Systems Are Required for Activation of the hmuO Promoter and Repression of the hemA Promoter in Corynebacterium diphtheriae
Infect. Immun., May 1, 2007; 75(5): 2421 - 2431.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
T. L. Campbell, C. S. Ederer, A. Allali-Hassani, and E. D. Brown
Isolation of the rstA Gene as a Multicopy Suppressor of YjeE, an Essential ATPase of Unknown Function in Escherichia coli
J. Bacteriol., April 15, 2007; 189(8): 3318 - 3321.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. Wu, F. Mao, V. Olman, and Y. Xu
Hierarchical classification of functionally equivalent genes in prokaryotes
Nucleic Acids Res., April 1, 2007; 35(7): 2125 - 2140.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Fleischer, R. Heermann, K. Jung, and S. Hunke
Purification, Reconstitution, and Characterization of the CpxRAP Envelope Stress System of Escherichia coli
J. Biol. Chem., March 23, 2007; 282(12): 8583 - 8593.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
Z. Ma and J.-R. Zhang
RR06 Activates Transcription of spr1996 and cbpA in Streptococcus pneumoniae
J. Bacteriol., March 15, 2007; 189(6): 2497 - 2509.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
N. C. Reading, A. G. Torres, M. M. Kendall, D. T. Hughes, K. Yamamoto, and V. Sperandio
A Novel Two-Component Signaling System That Activates Transcription of an Enterohemorrhagic Escherichia coli Effector Involved in Remodeling of Host Actin
J. Bacteriol., March 15, 2007; 189(6): 2468 - 2476.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
Y. A. Golubeva and J. M. Slauch
Salmonella enterica Serovar Typhimurium Periplasmic Superoxide Dismutase SodCI Is a Member of the PhoPQ Regulon and Is Induced in Macrophages
J. Bacteriol., November 15, 2006; 188(22): 7853 - 7861.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
B.-K. Cho, E. M. Knight, and B. O. Palsson
Transcriptional regulation of the fad regulon genes of Escherichia coli by ArcA.
Microbiology, August 1, 2006; 152(Pt 8): 2207 - 2219.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
J. G. Kusters, A. H. M. van Vliet, and E. J. Kuipers
Pathogenesis of Helicobacter pylori Infection
Clin. Microbiol. Rev., July 1, 2006; 19(3): 449 - 490.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
B. Venkatesh, L. Babujee, H. Liu, P. Hedley, T. Fujikawa, P. Birch, I. Toth, and S. Tsuyumu
The Erwinia chrysanthemi 3937 PhoQ Sensor Kinase Regulates Several Virulence Determinants.
J. Bacteriol., April 1, 2006; 188(8): 3088 - 3098.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
C. Nadler, Y. Shifrin, S. Nov, S. Kobi, and I. Rosenshine
Characterization of Enteropathogenic Escherichia coli Mutants That Fail To Disrupt Host Cell Spreading and Attachment to Substratum
Infect. Immun., February 1, 2006; 74(2): 839 - 849.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
F. Mika and R. Hengge
A two-component phosphotransfer network involving ArcB, ArcA, and RssB coordinates synthesis and proteolysis of {sigma}S (RpoS) in E. coli
Genes & Dev., November 15, 2005; 19(22): 2770 - 2781.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2004 by the American Society for Biochemistry and Molecular Biology.