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Papers In Press, published online ahead of print February 8, 2005
Department of Medicine and Clinical Science, Endocrinology and Metabolism, Kyoto University, Kyoto, Kyoto 606-8507
Corresponding Author: hiwaku{at}kuhp.kyoto-u.ac.jp
We developed and analyzed two types of transgenic mice: rat insulin II promoter–ghrelin transgenic (RIP-G Tg) and rat glucagon promoter-ghrelin transgenic mice (RGP-G Tg). The pancreatic tissue ghrelin concentration measured by C-terminal RIA and plasma des-acyl ghrelin concentration of RIP-G Tg were about 1,000 times and 3.4 times higher than those of nontransgenic littermates, respectively. The pancreatic tissue n-octanoylated ghrelin concentration measured by N-terminal RIA and plasma active ghrelin concentration of RIP-G Tg were not distinguishable from those of nontransgenic littermates. RIP-G Tg showed suppression of glucose-stimulated insulin secretion. Arginine-stimulated insulin secretion, pancreatic insulin mRNA and peptide levels, beta cell mass, islet architecture, GLUT2 and PDX-1 immunoreactivity in RIP-G Tg pancreas were not significantly different from those of nontransgenic littermates. Islet batch incubation study did not show suppression of insulin secretion of RIP-G Tg in vitro. Insulin tolerance test showed lower tendency of blood glucose levels in RIP-G Tg. Taking lower tendency of triglyceride level of RIP-G Tg into consideration, these results may indicate that the suppression of insulin secretion is likely due to the effect of des-acyl ghrelin on insulin sensitivity. RGP-G Tg, in which the pancreatic tissue ghrelin concentration measured by C-RIA was about 50 times higher than that of nontransgenic littermates, showed no significant changes in insulin secretion, glucose metabolism, islet mass and islet architecture. The present study raises the possibility that des-acyl ghrelin may have influence on glucose metabolism.
J. Biol. Chem, 10.1074/jbc.M411358200
Submitted on October 5, 2004
Revised on February 8, 2005
Accepted on February 8, 2005
Analysis of rat insulin II promoter-ghrelin transgenic mice and rat glucagon promoter-ghrelin transgenic mice
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