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M412476200v1
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Papers In Press, published online ahead of print May 11, 2005
J. Biol. Chem, 10.1074/jbc.M412476200
Submitted on November 4, 2004
Revised on May 9, 2005
Accepted on May 11, 2005

Double-spanning plant viral movement protein integration into the ER membrane is SRP-dependent, translocon-mediated, and concerted

Ana Saurí, Suraj Saksena, Jesús Salgado, Arthur E. Johnson, and Ismael Mingarro

Department of Biochemistry and Molecular Biology, University of Valencia, Burjassot, Valencia E-46 100

Corresponding Author: Ismael.Mingarro{at}uv.es

The current model for cell-to-cell movement of plant viruses holds that transport requires virus-encoded movement proteins that intimately associate with endoplasmic reticulum (ER) membranes. Here we have examined the early stages of the integration into ER membranes of a double-spanning viral movement protein using photocrosslinking. We have discovered that this process is cotranslational and proceeds in an SRP-dependent manner. In addition, nascent chain photocrosslinking to Sec61a and TRAM reveals that viral membrane protein insertion takes place via the translocon, as with most eukaryotic membrane proteins, but that the two transmembrane (TM) segments of the viral protein leave the translocon and enter the lipid bilayer together.


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