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Papers In Press, published online ahead of print January 6, 2005
Transcription Laboratory, Marie Curie Research Institute, Oxted, Surrey RH8 0TL
Corresponding Author: t.oelgeschlager{at}mcri.ac.uk
The TFIID complex is composed of the TATA-binding protein (TBP) and TBP-associated factors (TAFs) and is the only component of the general RNA polymerase II (RNAP II) transcription machinery with intrinsic sequence-specific DNA binding activity. Binding of TFIID to the core promoter region of protein-coding genes is a key event in RNAP II transcription activation as it is the first and rate-limiting step of transcription initiation complex assembly. Intense research efforts in the past have established that TFIID promoter binding activity as well as the function of TFIID/promoter complexes is tightly regulated through dynamic TFIID interactions with positive- and negative-acting transcription regulatory proteins.However, very little is known about the role of posttranslational modifications in the regulation of TFIID. Here we show that the human TFIID subunits hsTAF5 and hsTAF12 are modified by the small ubiquitin-related modifier SUMO-1 in vitro and in human cells. We identify K14 in hsTAF5 and K19 in hsTAF12 as the primary SUMO-1 acceptor sites and show that SUMO conjugation has no detectable effect on nuclear import or intranuclear distribution of hsTAF5 and hsTAF12. Finally, we demonstrate that purified human TFIID complex can be SUMO-1 modified in vitro at both hsTAF5 and hsTAF12. We find that SUMO-1 conjugation at hsTAF5 interferes with binding of TFIID to promoter DNA, whereas modification of hsTAF12 has no detectable effect on TFIID promoter binding activity. Our observations suggest that reversible SUMO modification at hsTAF5 contributes to the dynamic regulation of TFIID promoter binding activity in human cells.
J. Biol. Chem, 10.1074/jbc.M414149200
Submitted on December 16, 2004
Revised on January 6, 2005
Accepted on January 6, 2005
Sumo-1 modification of human TFIID complex subunits: Inhibition of TFIID promoter binding activity through sumo-1 modification of hsTAF5
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