JBC Advanced Glycation Endproducts

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on July 22, 2005
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
280/29/26953    most recent
M502614200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lu, K. V.
Right arrow Articles by Mischel, P. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lu, K. V.
Right arrow Articles by Mischel, P. S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print May 20, 2005
J. Biol. Chem, 10.1074/jbc.M502614200
Submitted on March 9, 2005
Revised on May 16, 2005
Accepted on May 20, 2005

Differential induction of glioblastoma migration and growth by two forms of pleiotrophin

Kan V. Lu, Kimberly A. Jong, Gloria Y. Kim, Jatinder Singh, Ederlyn Q. Dia, Koji Yoshimoto, Yinglin Wang, Timothy F. Cloughesy, Stanley F. Nelson, and Paul S. Mischel

Pathology and Laboratory Medicine, UCLA School of Medicine, Los Angeles, CA 90095-1732

Corresponding Author: pmischel{at}mednet.ucla.edu

Glioblastoma is the most common malignant brain tumor of adults and one of the most lethal cancers. The secreted growth factor pleiotrophin (PTN) promotes glioblastoma migration and proliferation, initiating its oncogenic activities through two cell surface receptors, the protein tyrosine phosphatase receptor zeta (PTPRZ1) and the anaplastic lymphoma kinase (ALK), respectively. Here, we report on the presence and purification of two naturally occurring forms of PTN (18 kDa and a 15 kDa) that differentially promote glioblastoma migration and proliferation. Using a panel of glioblastoma cell lines, including low passage patient-derived cultures, we demonstrate that PTN15 promotes glioblastoma proliferation in an ALK-dependent fashion, whereas immobilized PTN18 promotes haptotactic migration of glioblastoma cells in a PTPRZ1-dependent fashion. Mass spectrometric analysis indicated that PTN15 differs from PTN18 by processing of 12 C-terminal amino acids. To demonstrate clinical relevance, we show that PTN15, PTN18, and PTPRZ1 are significantly overexpressed in glioblastoma relative to normal brain at both mRNA and protein levels using microarray, western blot, and tissue microarray analyses on human tumors. These results indicate that the PTN18-PTPRZ1 and the PTN15-ALK signaling pathways represent potentially important therapeutic targets for glioblastoma invasion and growth.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Clin. Cancer Res.Home page
A. J.F. Lazar, P. Das, D. Tuvin, B. Korchin, Q. Zhu, Z. Jin, C. L. Warneke, P. S. Zhang, V. Hernandez, D. Lopez-Terrada, et al.
Angiogenesis-Promoting Gene Patterns in Alveolar Soft Part Sarcoma
Clin. Cancer Res., December 15, 2007; 13(24): 7314 - 7321.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Perez-Pinera, W. Zhang, Y. Chang, J. A. Vega, and T. F. Deuel
Anaplastic Lymphoma Kinase Is Activated Through the Pleiotrophin/Receptor Protein-tyrosine Phosphatase beta/{zeta} Signaling Pathway: AN ALTERNATIVE MECHANISM OF RECEPTOR TYROSINE KINASE ACTIVATION
J. Biol. Chem., September 28, 2007; 282(39): 28683 - 28690.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
H. Chen, M. S. Gordon, R. A. Campbell, M. Li, C. S. Wang, H. J. Lee, E. Sanchez, S. J. Manyak, D. Gui, D. Shalitin, et al.
Pleiotrophin is highly expressed by myeloma cells and promotes myeloma tumor growth
Blood, July 1, 2007; 110(1): 287 - 295.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
P. Perez-Pinera, S. Alcantara, T. Dimitrov, J. A. Vega, and T. F. Deuel
Pleiotrophin disrupts calcium-dependent homophilic cell-cell adhesion and initiates an epithelial-mesenchymal transition
PNAS, November 21, 2006; 103(47): 17795 - 17800.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
W. Wan, M. S. Albom, L. Lu, M. R. Quail, N. C. Becknell, L. R. Weinberg, D. R. Reddy, B. P. Holskin, T. S. Angeles, T. L. Underiner, et al.
Anaplastic lymphoma kinase activity is essential for the proliferation and survival of anaplastic large-cell lymphoma cells
Blood, February 15, 2006; 107(4): 1617 - 1623.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. Y. Gouzi, C. Moog-Lutz, M. Vigny, and N. Brunet-de Carvalho
Role of the subcellular localization of ALK tyrosine kinase domain in neuronal differentiation of PC12 cells
J. Cell Sci., December 15, 2005; 118(24): 5811 - 5823.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Polytarchou, M. Hatziapostolou, and E. Papadimitriou
Hydrogen Peroxide Stimulates Proliferation and Migration of Human Prostate Cancer Cells through Activation of Activator Protein-1 and Up-regulation of the Heparin Affin Regulatory Peptide Gene
J. Biol. Chem., December 9, 2005; 280(49): 40428 - 40435.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.