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M504106200v1
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Papers In Press, published online ahead of print August 28, 2005
J. Biol. Chem, 10.1074/jbc.M504106200
Submitted on April 15, 2005
Revised on August 22, 2005
Accepted on August 28, 2005

Nuclear import of alpha B-crystallin is phosphorylation-dependent and hampered by hyperphosphorylation of the myopathy-related mutant R120G

John den Engelsman, Danny Gerrits, Wilfried W. de Jong, Jeffrey Robbins, Kanefusa Kato, and Wilbert C. Boelens

Department of Biochemistry, Radboud University of Nijmegen, Nijmegen 6500 HB

Corresponding Author: w.boelens{at}ncmls.ru.nl

Phosphorylation modulates the functioning of alphaB-crystallin as a molecular chaperone. We here explore the role of phosphorylation in the nuclear import and cellular localization of alphaB-crystallin in HeLa cells. Inhibition of nuclear export demonstrated that phosphorylation of alphaB-crystallin is required for import into the nucleus. As revealed by mutant analysis, phosphorylation at Ser-59 is crucial for nuclear import and phosphorylation at Ser-45 is required for speckle localization. Co-immunoprecipitation experiments suggested that the import of alphaB-crystallin is possibly regulated by its phosphorylation-dependent interaction with the survival motor neuron (SMN) protein, an important factor in snRNP nuclear import and assembly. This interaction was supported by colocalization of endogenous phosphorylated alphaB-crystallin with SMN in nuclear structures. The cardiomyopathy-causing alphaB-crystallin mutant R120G was found to be excessively phosphorylated, which disturbed SMN interaction and nuclear import, and resulted in the formation of cytoplasmic inclusions. Like for other protein aggregation disorders, hyperphosphorylation appears as an important aspect of the pathogenicity of alphaB-crystallin R120G.


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