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Papers In Press, published online ahead of print October 5, 2005
J. Biol. Chem, 10.1074/jbc.M504497200
Submitted on April 25, 2005
Revised on September 26, 2005
Accepted on October 5, 2005

Endocytosis resumes during late mitosis and is required for cytokinesis

Jill Kuglin Schweitzer, Erin E. Burke, Holly V. Goodson, and Crislyn D'Souza-Schorey

Biological Sciences, University of Notre Dame, Notre Dame, IN 46556-0369

Corresponding Author: D'Souza-Schorey.1{at}nd.edu

Recent work has underscored the importance of membrane trafficking events during cytokinesis. For example, targeted membrane secretion occurs at the cleavage furrow in animal cells and proteins that regulate endocytosis also influence the process of cytokinesis. Nonetheless, the prevailing dogma is that endosomal membrane trafficking ceases during mitosis and resumes after cell division is complete. In this study we have characterized endocytic membrane trafficking events during mammalian cell cytokinesis. We have found that while endocytosis ceases during the early stages of mitosis, it resumes during late mitosis in a temporally and spatially regulated pattern as cells progress from anaphase to cytokinesis. Using fixed- and live-cell imaging we have found that during cleavage furrow ingression vesicles are internalized from the polar region and subsequently trafficked to the midbody area during later stages of cytokinesis. In addition, we demonstrate that cytokinesis is inhibited when clathrin-mediated endocytosis is blocked, either genetically using a series of dominant negative mutants. In contrast to previous thought, we conclude that endocytosis resumes during the later stages of mitosis, before cytokinesis is completed. Furthermore, based on our findings, we propose that the proper regulation of endosomal membrane traffic is necessary for the successful completion of cytokinesis.


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