| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print September 26, 2005
Department of Biochemistry, University of Missouri-Columbia, Columbia, MO 65211
Corresponding Author: erbl{at}missouri.edu
Extracellular ATP and UTP induce chemotaxis, or directed cell migration, by stimulating the G protein-coupled P2Y2 nucleotide receptor (P2Y2R). Previously, we found that an arginine-glycine-aspartic acid (RGD) integrin-binding domain in the P2Y2R enables this receptor to interact selectively with
J. Biol. Chem, 10.1074/jbc.M504819200
Submitted on May 2, 2005
Revised on August 31, 2005
Accepted on September 26, 2005
The P2Y2 nucleotide receptor interacts with
v integrins to activate Go and induce cell migration
v
3 and v5 integrins, an interaction that is prevented by mutation of the RGD sequence to arginine-glycine-glutamic acid (RGE) (1). This RGD domain also was found to be necessary for coupling the P2Y2R to Go- but not Gq-mediated intracellular calcium mobilization, leading us to investigate the role of P2Y2R interaction with integrins in nucleotide-induced chemotaxis. Here we show that mutation of the RGD sequence to RGE in the human P2Y2R expressed in 1321N1 astrocytoma cells completely prevented UTP-induced chemotaxis as well as activation of Go, Rac, and Vav2, a guanine nucleotide exchange factor for Rac. UTP also increased expression of vitronectin, an extracellular matrix protein that is a ligand for v3/5 integrins, in cells expressing the wild-type but not the RGE mutant P2Y2R. P2Y2R-mediated chemotaxis, Rac and Vav2 activation, and vitronectin up-regulation were inhibited by pretreatment of the cells with anti-v5 integrin antibodies, v integrin antisense oligonucleotides, or the Gi/o inhibitor, pertussis toxin. Thus, the RGD-dependent interaction between the P2Y2R and v integrins is necessary for the P2Y2R to activate Go and to initiate Go-mediated signaling events leading to chemotaxis.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  N. Yu, L. Erb, R. Shivaji, G. A. Weisman, and C. I. Seye Binding of the P2Y2 Nucleotide Receptor to Filamin A Regulates Migration of Vascular Smooth Muscle Cells Circ. Res., March 14, 2008; 102(5): 581 - 588. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shen and P. E. DiCorleto ADP Stimulates Human Endothelial Cell Migration via P2Y1 Nucleotide Receptor-Mediated Mitogen-Activated Protein Kinase Pathways Circ. Res., February 29, 2008; 102(4): 448 - 456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Taboubi, J. Milanini, E. Delamarre, F. Parat, F. Garrouste, G. Pommier, J. Takasaki, J.-C. Hubaud, H. Kovacic, and M. Lehmann G{alpha}(q/11)-coupled P2Y2 nucleotide receptor inhibits human keratinocyte spreading and migration FASEB J, December 1, 2007; 21(14): 4047 - 4058. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Stokes and A. Surprenant Purinergic P2Y2 Receptors Induce Increased MCP-1/CCL2 Synthesis and Release from Rat Alveolar and Peritoneal Macrophages J. Immunol., November 1, 2007; 179(9): 6016 - 6023. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Liao, C. I. Seye, G. A. Weisman, and L. Erb The P2Y2 nucleotide receptor requires interaction with {alpha}v integrins to access and activate G12 J. Cell Sci., May 1, 2007; 120(9): 1654 - 1662. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
