JBC Origene Your Gene Company

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on September 23, 2005
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow Supplemental data
Right arrow All Versions of this Article:
280/38/32736    most recent
M505558200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Oka, T.
Right arrow Articles by Krieger, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oka, T.
Right arrow Articles by Krieger, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print July 28, 2005
J. Biol. Chem, 10.1074/jbc.M505558200
Submitted on May 20, 2005
Revised on July 15, 2005
Accepted on July 28, 2005

Genetic analysis of the subunit organization and function of the COG complex: Studies of Cog5 and Cog7 deficient mammalian cells

Toshihiko Oka, Eliza Vasile, Marsha Penman, Carl D. Novina, Derek M. Dykxhoorn, Daniel Ungar, Frederick M. Hughson, and Monty Krieger

Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139

Corresponding Author: krieger{at}mit.edu

The conserved oligomeric Golgi (COG) complex is an eight-subunit (Cog1-8) peripheral Golgi protein involved in Golgi-associated membrane trafficking and glycoconjugate synthesis. We have analyzed COG’s structure and function using Cog1- or Cog2-null CHO cell mutants, fibroblasts from a patient with Cog7 deficient congenital disorders of glycosylation and stable Cog5 deficient HeLa cells generated by RNA interference. While the dilation of some Golgi cisternae in Cog5 deficient cells resembled that observed in Cog1 or Cog2 deficient cells, their global glycosylation defects (less severe) and intracellular processing and function of LDL receptors (essentially normal) differed from Cog1 and Cog2 deficient cells. Immunoblotting, gel filtration and immunofluorescence microscopy analyses of the COG deficient cells and cell extracts indicated that 1) Cog2-4 and Cog5-7 form stable subcomplexes, 2) Cog1 mediates Golgi association of a Cog2-4 plus Cog8 subcomplex, 3) Cog8 associates stably with both Cog5-7 and Cog1-4 subcomplexes, and thus 4) Cog8 helps assemble the Cog1-4 and Cog5-7 subcomplexes into the complete COG complex. Only one or two of the seven Cog1- or Cog2-dependent Golgi membrane proteins called GEARs are also sensitive to Cog5 or Cog7 deficiency, indicating that the COG subunits play distinctive roles in controlling Golgi structure and function.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 GeneticsHome page
W. Peng, C. Togawa, K. Zhang, and S. K. Kurdistani
Regulators of Cellular Levels of Histone Acetylation in Saccharomyces cerevisiae
Genetics, May 1, 2008; 179(1): 277 - 289.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
A. Shestakova, E. Suvorova, O. Pavliv, G. Khaidakova, and V. Lupashin
Interaction of the conserved oligomeric Golgi complex with t-SNARE Syntaxin5a/Sed5 enhances intra-Golgi SNARE complex stability
J. Cell Biol., December 17, 2007; 179(6): 1179 - 1192.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. F. Cavanaugh, X. Chen, B. C. Richardson, D. Ungar, I. Pelczer, J. Rizo, and F. M. Hughson
Structural Analysis of Conserved Oligomeric Golgi Complex Subunit 2
J. Biol. Chem., August 10, 2007; 282(32): 23418 - 23426.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
F. Foulquier, D. Ungar, E. Reynders, R. Zeevaert, P. Mills, M. T. Garcia-Silva, P. Briones, B. Winchester, W. Morelle, M. Krieger, et al.
A new inborn error of glycosylation due to a Cog8 deficiency reveals a critical role for the Cog1-Cog8 interaction in COG complex formation
Hum. Mol. Genet., April 1, 2007; 16(7): 717 - 730.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
C. Kranz, B. G. Ng, L. Sun, V. Sharma, E. A. Eklund, Y. Miura, D. Ungar, V. Lupashin, R. D. Winkel, J. F. Cipollo, et al.
COG8 deficiency causes new congenital disorder of glycosylation type IIh
Hum. Mol. Genet., April 1, 2007; 16(7): 731 - 741.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F. Foulquier, E. Vasile, E. Schollen, N. Callewaert, T. Raemaekers, D. Quelhas, J. Jaeken, P. Mills, B. Winchester, M. Krieger, et al.
Conserved oligomeric Golgi complex subunit 1 deficiency reveals a previously uncharacterized congenital disorder of glycosylation type II.
PNAS, March 7, 2006; 103(10): 3764 - 3769.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Ungar, T. Oka, E. Vasile, M. Krieger, and F. M. Hughson
Subunit Architecture of the Conserved Oligomeric Golgi Complex
J. Biol. Chem., September 23, 2005; 280(38): 32729 - 32735.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.