JBC INTERFERin siRNA transfection reagent

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on March 24, 2006 Originally published In Press as doi:10.1074/jbc.M510016200 on December 1, 2005
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
281/12/8024    most recent
M510016200v2
M510016200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fong, S. S
Right arrow Articles by Sauer, U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fong, S. S
Right arrow Articles by Sauer, U.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print November 30, 2005
J. Biol. Chem, 10.1074/jbc.M510016200
Submitted on September 12, 2005
Revised on November 28, 2005
Accepted on November 30, 2005

Latent pathway activation and increased pathway capacity enable Escherichia Coli adaptation to loss of key Ketabolic Enzymes

Stephen S Fong, Annik Nanchen, Bernhard O Palsson, and Uwe Sauer

Chemical and Life Science Engineering, Virginia Commonwealth University, Richmond, VA 23284

Corresponding Author: ssfong{at}vcu.edu

The ability of biological systems to adapt to genetic and environmental perturbations is a fundamental but poorly understood process at the molecular level. By quantifying metabolic fluxes and global mRNA abundance, we investigated the genetic and metabolic mechanisms that underlie adaptive evolution of four metabolic gene deletion mutants of Escherichia coli (pgi, ppc, pta, tpi) in parallel evolution experiments of each mutant. The initial response to the gene deletions was flux rerouting through local bypass reactions or normally latent pathways. The principal effect of evolution was improved capacity of already active pathways, while new flux distributions were not observed. Combinatorial changes in capacity and pathway activation, however, led to different intracellular flux states that enabled evolution in 3 of the 4 parallel cases tested. The molecular bases of the evolved phenotypes was then elucidated by global mRNA transcript analyses. Activation of latent pathways and flux changes in the tricarboxylic acid cycle were found to correlate well with molecular changes at the transcriptional level. Flux alterations in other central metabolic pathways, in contrast, were apparently not connected to changes in the transcriptional network. These results give new insight into the dynamics of the evolutionary process by demonstrating the flexibility of the metabolic network of E. coli to compensate for genetic perturbations and the utility of combining multiple high-throughput data sets to differentiate between causal and non-causal mechanistic changes.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
A. Nanchen, A. Schicker, O. Revelles, and U. Sauer
Cyclic AMP-Dependent Catabolite Repression Is the Dominant Control Mechanism of Metabolic Fluxes under Glucose Limitation in Escherichia coli
J. Bacteriol., April 1, 2008; 190(7): 2323 - 2330.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
Q. Hua, A. R. Joyce, B. O. Palsson, and S. S. Fong
Metabolic Characterization of Escherichia coli Strains Adapted to Growth on Lactate
Appl. Envir. Microbiol., July 15, 2007; 73(14): 4639 - 4647.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
N. Ishii, K. Nakahigashi, T. Baba, M. Robert, T. Soga, A. Kanai, T. Hirasawa, M. Naba, K. Hirai, A. Hoque, et al.
Multiple High-Throughput Analyses Monitor the Response of E. coli to Perturbations
Science, April 27, 2007; 316(5824): 593 - 597.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.