JBC Anatrace, Inc.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on April 21, 2006
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
281/16/11097    most recent
M511460200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Filipeanu, C. M
Right arrow Articles by Wu, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Filipeanu, C. M
Right arrow Articles by Wu, G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print February 16, 2006
J. Biol. Chem, 10.1074/jbc.M511460200
Submitted on October 21, 2005
Accepted on February 16, 2006

Enhancement of the recycling and activation of beta -adrenergic receptor by Rab4 GTPase in cardiac myocytes

Catalin M Filipeanu, Fuguo Zhou, May L Lam, Kenneth E Kerut, William C Claycomb, and Guangyu Wu

Pharmacology, Louisiana State University Health Sciences Center, New Orleans, LA 70112

Corresponding Author: gwu{at}lsuhsc.edu

We investigate the role of Rab4, a Ras-like small GTPase coordinating protein transport from the endosome to the plasma membrane, on the recycling and activation of endogenous beta-adrenergic receptor (beta-AR) in HL-1 cardiac myocytes in vitro and transgenic mouse hearts in vivo. beta1-AR the predominant subtype of beta-AR in HL-1 cardiac myocytes, is internalized after stimulation with isoproterenol (ISO) and fully recycled at 4 hrs upon ISO removal. Transient expression of Rab4 markedly facilitated recycling of internalized beta-AR to the cell surface and enhanced beta-AR signaling as measured by ISO-stimulated cAMP production. Transgenic overexpression of Rab4 in the mouse myocardium significantly increased the number of beta-AR in the plasma membrane and augmented cAMP production at the basal level and in response to ISO stimulation. Rab4 overexpression induced concentric cardiac hypertrophy with a moderate increase in ventricle/body weight ratio and posterior wall thickness and a selective upregulation of the beta-myosin heavy chain gene. These data provide the first evidence indicating that Rab4 is a rate-limiting factor for the recycling of endogenous beta-AR and augmentation of Rab4-mediated traffic enhances beta-AR function in cardiac myocytes.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
E. Schonteich, G. M. Wilson, J. Burden, C. R. Hopkins, K. Anderson, J. R. Goldenring, and R. Prekeris
The Rip11/Rab11-FIP5 and kinesin II complex regulates endocytic protein recycling
J. Cell Sci., November 15, 2008; 121(22): 3824 - 3833.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
A. D. Zadeh, H. Xu, M. E. Loewen, G. P. Noble, D. F. Steele, and D. Fedida
Internalized Kv1.5 traffics via Rab-dependent pathways
J. Physiol., October 15, 2008; 586(20): 4793 - 4813.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. P. McEwen, S. M. Schumacher, Q. Li, M. D. Benson, J. A. Iniguez-Lluhi, K. M. Van Genderen, and J. R. Martens
Rab-GTPase-dependent Endocytic Recycling of KV1.5 in Atrial Myocytes
J. Biol. Chem., October 5, 2007; 282(40): 29612 - 29620.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
A. S. Tutor, P. Penela, and F. Mayor Jr.
Anti-{beta}1-adrenergic receptor autoantibodies are potent stimulators of the ERK1/2 pathway in cardiac cells
Cardiovasc Res, October 1, 2007; 76(1): 51 - 60.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
C. Ding, B. Walcott, and K. T. Keyser
The {alpha}1- and {beta}1-Adrenergic Modulation of Lacrimal Gland Function in the Mouse
Invest. Ophthalmol. Vis. Sci., April 1, 2007; 48(4): 1504 - 1510.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2006 by the American Society for Biochemistry and Molecular Biology.