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Papers In Press, published online ahead of print March 13, 2006
Pharmacology and Toxicology, University of Graz, Graz, Styria A-8010
Corresponding Author: klaus.groschner{at}kfunigraz.ac.at
Canonical transient receptor potential proteins (TRPC) have been proposed to form homo- or heteromeric cation channels in a variety of tissues including the vascular endothelium. Assembly of TRPC multimers is incompletely understood. In particular heteromeric assembly of distantly related TRPC isoforms is still a controversial issue. As we have previously suggested TRPC proteins as basis of the redox-activated cation conductance of porcine aortic endothelial cells (PAEC), we set out to analyze the TRPC subunit composition of endogenous endothelial TRPC channels and report here on a redox-sensitive TRPC3/TRPC4 channel complex. The ability of TRPC3 and TRPC4 proteins to associate and to form a cation conducting pore complex was supported by four lines of evidence: 1) Co-immunoprecipitation experiments in PAEC and in HEK293 cells demonstrated the association of TRPC3 and TRPC4 in the same complex. 2) FRET analysis demonstrated TRPC3/TRPC4 association, involving close proximity between the N-terminus of TRPC4 and the C-terminus of TRPC3 subunits. 3) Co-expression of TRPC3 and TRPC4 in HEK293 cells generated a channel that displayed distinct biophysical and regulatory properties. 4) Expression of dominant-negative TRPC4 proteins suppressed TRPC3-related channel activity in the HEK293 expression system and in native endothelial cells. Specifically, an extracellularly hemagglutinin (HA)-tagged TRPC4 mutant that is sensitive to block by anti-HA-antibody was found to transfer anti-HA sensitivity to both TRPC3-related currents in the HEK293 expression system and the redox-sensitive cation conductance of PAECs. We propose TRPC3 and TRPC4 as subunits of native endothelial cation channels that are governed by the cellular redox state.
J. Biol. Chem, 10.1074/jbc.M512205200
Submitted on November 14, 2005
Revised on March 6, 2006
Accepted on March 13, 2006
Trpc3 and trpc4 associate to form a redox-sensitive cation channel-evidence for expression of native trpc3/trpc4 heteromeric channels in endothelial cells
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