A structural model of the cytochrome c reductase / oxidase supercomplex from yeast mitochondria

doi:10.1074/jbc.M610545200

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Papers In Press, published online ahead of print February 23, 2007
J. Biol. Chem, 10.1074/jbc.M610545200
Submitted on November 13, 2006
Revised on January 29, 2007
Accepted on February 23, 2007

A structural model of the cytochrome c reductase / oxidase supercomplex from yeast mitochondria

Jesco Heinemeyer, Hans Peter Braun, Egbert J. Boekema, and Roman Kouril

Biophysical Chemistry, University of Groningen, Groningen 9747 AG

Corresponding Author: r.kouril{at}rug.nl

Mitochondrial respiratory chain complexes are arranged in supercomplexes within the inner membrane. Interaction of cytochrome c reductase (complex III) and cytochrome c oxidase (complex IV) was investigated in Saccharomyces cerevisiae. Projection maps at 15 Å resolution of supercomplexes III₂+IV₁ and III₂+IV₂ were obtained by electron microscopy. Based on a comparison of our maps with atomic X-ray structures for complexes III and IV we present a pseudo-atomic model of their precise interaction. Two complex IV monomers are specifically attached to dimeric complex III with their convex sides. The opposite sides, which represent the complex IV dimer interface in the X-ray structure, are open for complex IV - complex IV interactions. This could lead to oligomerization of III₂+IV₂ supercomplexes, but this was not detected. Instead, binding of cytochrome c to the supercomplexes was revealed. It was calculated that cytochrome c has to move less than 40 Å at the surface of the supercomplex for electron transport between complex III₂ and complex IV. Hence, the prime function of the supercomplex III₂+IV₂ is proposed to be a scaffold for effective electron transport between complexes III and IV.

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