JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on March 9, 2007
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
282/10/7668    most recent
M610946200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ranjith-Kumar, C. T.
Right arrow Articles by Kao, C. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ranjith-Kumar, C. T.
Right arrow Articles by Kao, C. C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print January 5, 2007
J. Biol. Chem, 10.1074/jbc.M610946200
Submitted on November 28, 2006
Revised on December 28, 2006
Accepted on January 5, 2007

Biochemical and functional analyses of the human toll-like receptor 3 ectodomain

C. T. Ranjith-Kumar, William Miller, Jin Xiong, William K. Russell, Roberta Lamb, Jonathan Santos, Karen E. Duffy, Larissa Cleveland, Mary Park, Kanchan Bhardwaj, Zhaoxiang Wu, David H. Russell, Robert T. Sarisky, M. Lamine Mbow, and C. Cheng Kao

Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843

Corresponding Author: ckao{at}tamu.edu

The structure of the human Toll-like receptor 3 (TLR3) ectodomain (ECD) was recently solved by X-ray crystallography, leading to a number of models concerning TLR3 function (Choe, J., Kelker, M. S., and Wilson, I. A. (2005). Science 309, 581-585 and Bell, J. K., Botos, I., Hall, P. R., Askins, J., Shiloach, J., Segal, D. M., and Davies, D. R. (2005) Proc Natl Acad Sci USA 102, 10976-10980) The structure revealed four pairs of cysteines that are putatively involved in disulfide bond formation, several residues that are predicted to be involved in dimerization between ECD subunits, and surfaces that could bind to poly(I:C). In addition, there are two loops that protrude from the central solenoid structure of the protein. We examined the recombinant TLR3 ECD for disulfide bond formation, poly(I:C) binding, and protein-protein interaction. We also made over 80 mutations in the residues that could affect these features in the full-length TLR3 were examined their effects in TLR3-mediated NF-kB activation. A number of mutations that affected TLR3 activity also affected the ability to act as dominant negative inhibitors of wild-type TLR3. Loss of putative RNA binding did not necessarily affect dominant negative activity. All of the results support a model where a dimer of TLR3 is the form that binds RNA and activates signal transduction.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
K. Fukuda, T. Watanabe, T. Tokisue, T. Tsujita, S. Nishikawa, T. Hasegawa, T. Seya, and M. Matsumoto
Modulation of Double-stranded RNA Recognition by the N-terminal Histidine-rich Region of the Human Toll-like Receptor 3
J. Biol. Chem., August 15, 2008; 283(33): 22787 - 22794.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. T. Ranjith-Kumar, K. E. Duffy, J. L. Jordan, A. Eaton-Bassiri, R. Vaughan, S. A. Hoose, R. J. Lamb, R. T. Sarisky, and C. C. Kao
Single-Stranded Oligonucleotides Can Inhibit Cytokine Production Induced by Human Toll-Like Receptor 3
Mol. Cell. Biol., July 15, 2008; 28(14): 4507 - 4519.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
A. O. Edwards, D. Chen, B. L. Fridley, K. M. James, Y. Wu, G. Abecasis, A. Swaroop, M. Othman, K. Branham, S. K. Iyengar, et al.
Toll-like Receptor Polymorphisms and Age-Related Macular Degeneration
Invest. Ophthalmol. Vis. Sci., April 1, 2008; 49(4): 1652 - 1659.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. N. Leonard, R. Ghirlando, J. Askins, J. K. Bell, D. H. Margulies, D. R. Davies, and D. M. Segal
The TLR3 signaling complex forms by cooperative receptor dimerization
PNAS, January 8, 2008; 105(1): 258 - 263.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
S.-Y. Zhang, E. Jouanguy, S. Ugolini, A. Smahi, G. Elain, P. Romero, D. Segal, V. Sancho-Shimizu, L. Lorenzo, A. Puel, et al.
TLR3 Deficiency in Patients with Herpes Simplex Encephalitis
Science, September 14, 2007; 317(5844): 1522 - 1527.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. T. Ranjith-Kumar, W. Miller, J. Sun, J. Xiong, J. Santos, I. Yarbrough, R. J. Lamb, J. Mills, K. E. Duffy, S. Hoose, et al.
Effects of Single Nucleotide Polymorphisms on Toll-like Receptor 3 Activity and Expression in Cultured Cells
J. Biol. Chem., June 15, 2007; 282(24): 17696 - 17705.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2007 by the American Society for Biochemistry and Molecular Biology.