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A more recent version of this article appeared on June 22, 2007 Originally published In Press as doi:10.1074/jbc.M700708200 on March 28, 2007
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Papers In Press, published online ahead of print March 29, 2007
J. Biol. Chem, 10.1074/jbc.M700708200
Submitted on January 24, 2007
Revised on January 1, 1998
Accepted on March 28, 2007

Ceramides and sphingomyelins with high proportions of very long-chain polyunsaturated fatty acids in mammalian germ cells

Natalia Edith Furland, Samanta Romina Zanetti, Gerardo Martín Oresti, Eduardo Néstor Maldonado, and Marta Isabel Aveldaño

Instituto de Investigaciones Bioquímicas Bahía Blanca, Bahía Blanca, Buenos Aires 8000

Corresponding Author: avelda{at}criba.edu.ar

Very long-chain polyunsaturated fatty acids (VLCPUFA) have previously been shown to be components of sphingomyelin (SM) of mam-malian testis and spermatozoa. Here we exam-ined the fatty acids of testicular ceramide (Cer) in comparison with those of SM in some mam-mals with a special focus on the rat testis. In bull, cat, dog, rabbit, mouse, and rat, VLCPUFA were found in both testicular lipids, Cer having a higher percentage of VLCPUFA than SM. Rat testis had the highest percentage of VLCPUFA in both lipids, the major ones being 28:4n-6 and 30:5n-6. VLCPUFA-containing SM and Cer occurred in cells lo-cated in the seminiferous tubules, where germ cells had a higher percentage of these species than Sertoli cells. Seminiferous tubule frac-tionation showed that SM and Cer of mito-chondria and lysosomes had mostly saturates and negligible VLCPUFA, the latter being im-portant in the SM and Cer of microsomes and other membrane fractions. VLCPUFA were absent from the SM and Cer of rat prepuberal testis, increased with the onset of spermato-genesis to account for nearly 15% and 40% of the total fatty acids of testicular SM and Cer, respectively, remained at those levels through-out the adult life of fertile rats, and tended to decrease at advanced ages. Four conditions that lead to selective death of germ cells in vivo, namely experimental cryptorchidism, post-ischemic reperfusion, focalized X-ray irradia-tion and treatments with the antineoplasic drug doxorubicin, caused the VLCPUFA to disap-pear from the testicular SM and Cer of adult fertile rats, showing that these lipids are specific traits of spermatogenic cells.


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