Epidermal Growth Factor and a New Derivative
RAPID ISOLATION PROCEDURES AND BIOLOGICAL AND CHEMICAL CHARACTERIZATION
- From the Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232
Abstract
A new procedure has been devised for the preparation of pure epidermal growth factor in high yield. The method involves a two-step column fractionation of crude extracts of the submaxillary gland obtained from adult male mice. Biologically active epidermal growth factor lacking the COOH-terminal Leu-Arg residues was also isolated.
Footnotes
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- Received June 26, 1972.
- © 1972, by the American Society of Biological Chemists, Inc.
