Isolation of collagen binding proteins from embryonic chicken corneal epithelial cells.

Abstract

In the present paper, we report the isolation and characterization of embryonic corneal membrane glycoproteins that demonstrate specific affinity for collagen. Two collagen binding proteins have been isolated: a novel 70-kDa protein and a 47-kDa protein which is apparently similar to that reported by Kurkinen et al. (Kurkinen, M., Taylor, A., Garrels, J. I., and Hogan, B. (1984) J. Biol. Chem. 259, 5915-5922). Both proteins label metabolically with [35S]methionine and [3H] glucosamine. 125I iodination of cell surface proteins revealed that the two collagen binding proteins are expressed on the epithelial cell surface. The 70-kDa protein appears to be an integral membrane protein, whereas the 47-kDa protein can be removed from membranes by alkali treatment. The isolated proteins exhibit binding to native type IV collagen as well as heat-denatured type I collagen. It seems likely that we have isolated, at least in part, the cell surface receptor or receptor complex that binds collagen to the basal surface of epithelia.