A Neuronal Protein Tyrosine Phosphatase Induced by Nerve Growth Factor (*)
- § To whom reprint requests should be addressed: Child Study Center, Yale University School of Medicine, 230 S. Frontage Rd., New Haven, CT 06520-7900. Tel.: 203-737-2224; Fax: 203-785-7611.
Abstract
A new protein tyrosine phosphatase (PC12-PTP1) was identified in nerve growth factor (NGF)-treated PC12 cells. The mRNA level of PC12-PTP1 is increased 9-fold over the initial 8 h of NGF treatment and then decreases dramatically after 24 h of treatment. In rat brain, three transcripts corresponding to 1.5, 2.6, and 3.0 kilobases (kb) in size are detected by Northern blot analysis. Although the 1.5- and 2.6-kb transcripts are present in brain and other tissues, the 3-kb transcript is exclusively expressed in brain and the expression of this transcript alone increases following NGF treatment. PC12-PTP1 is a non-receptor protein tyrosine phosphatase (PTP) with a 50% sequence homology in the phosphatase domain with several other non-receptor PTPs. PC12-PTP1 fusion protein exhibits tyrosine phosphatase activity, and in vitro translation of the PC12-PTP1 transcript produces a major protein of 39 kDa. The data presented suggest that NGF regulates the expression of PC12-PTP1 during periods of neuronal growth and differentiation.
Footnotes
-
↵* This work was supported by NIMH Grants MH00856 and MH49351, and the NARSAD Foundation (to P. J. L.), and NIMH Postdoctoral Training Fellowship Grant MH18268 (to E. S.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBank™/EMBL Data Bank with accession number(s) U14914[GenBank].
-
↵1 The abbreviations used are:
- NGF
-
nerve growth factor
- PTK
-
protein tyrosine kinase
- PTP
-
protein tyrosine phosphatase
- STEP
-
striatum-enriched phosphatase
- MAP
-
mitogen-activated protein
- pNPP
-
para-nitrophenyl phosphate
- kb
-
kilobase(s)
- bp
-
base pair(s).
-
- Received July 25, 1994.
- Revision received November 2, 1994.
- © 1995 by The American Society for Biochemistry and Molecular Biology, Inc.
