The Human 180-kDa Receptor for Secretory Phospholipases A
MOLECULAR CLONING, IDENTIFICATION OF A SECRETED SOLUBLE FORM, EXPRESSION, AND CHROMOSOMAL LOCALIZATION (*)
- From the (1) Institut de Pharmacologie Moléculaire et Cellulaire, 660 route des Lucioles, Sophia Antipolis, 06560 Valbonne, France and the
- Unité de Génétique Médicale et Développement, Inserm U406, Faculté de Médecine, 27, Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
- § To whom correspondence should be addressed: Institut de Pharmacologie Moléculaire et Cellulaire, UPR 411 du CNRS, 660 route des Lucioles, Sophia Antipolis, 06560 Valbonne, France. Tel.: 33-93957700/02; Fax: 33-93957704.
Abstract
Secretory phospholipases A2(sPLA2) are structurally related enzymes found in mammals as well as in insect and snake venoms. They have been associated with several physiological, pathological, and toxic processes. Some of these effects are apparently linked to the existence of specific receptors for both venom and mammalian sPLA2s. We report here the molecular cloning and expression of one of these sPLA2receptors from human kidney. Two transcripts were detected. One encodes for a transmembrane form of the sPLA2receptor and the other one is an alternatively processed transcript, caused by polyadenylation occurring at a site within an intron in the C terminus part of the transcriptional unit. This transcript encodes for a shortened secreted soluble sPLA2receptor lacking the coding region for the transmembrane segment. Quantitative polymerase chain reaction experiments indicate a 1.6:1 ratio between the levels of transcripts encoding for the membrane-bound and soluble forms of the receptor, respectively. Soluble and membrane-bound human sPLA2receptors both bind sPLA2with high affinities. However, the binding properties of the human receptors are different from those obtained with the rabbit membrane-bound sPLA2receptor. The 180-kDa human sPLA2receptor gene has been mapped in the q23-q24 bands of chromosome 2.
Footnotes
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↵* This work was supported by the Centre National de la Recherche Scientifique (CNRS), the Association pour la Recherche sur le Cancer (ARC), and Ministère de la Défense Nationale Grant DRET 93/122. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBank/EMBL Data Bank with accession number(s) U17033 and U17034.
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↵1 The abbreviations used are:
- sPLA2
-
secretory phospholipase A2
- svPLA2
-
snake venom phospholipase A2
- OS1
-
Oxyuranus scutellatus scutellatus toxin 1
- OS2
-
Oxyuranus scutellatus scutellatus toxin 2
- CRD
-
carbohydrate recognition domain
- BSA
-
bovine serum albumin
- PCR
-
polymerase chain reaction
- RT-PCR
-
reverse transcriptase polymerase chain reaction
- bp
-
base pair(s)
- kbp
-
kilobase pair(s).
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↵2J. P. Nicolas, P. Ancian, E. Zvaritch, G. Lambeau, and M. Lazdunski, manuscript in preparation.
- © 1995 by The American Society for Biochemistry and Molecular Biology, Inc.
