The Gene Structure and Organization of Mouse PG-M, a Large Chondroitin Sulfate Proteoglycan
GENOMIC BACKGROUND FOR THE GENERATION OF MULTIPLE PG-M TRANSCRIPTS (*)
- From the (1) Institute for Molecular Science of Medicine, Aichi Medical University, Nagakute, Aichi 480-11, Japan
- § To whom correspondence should be addressed. Tel.: 0561-62-3311 (ext. 2088); Fax: 0561-63-3532.
Abstract
We previously showed not only the presence of multiple RNA transcripts of different sizes encoding the core protein of mouse PG-M, but also their tissue-dependent expression. Major causes for the multiple forms were found to be due to alternative usage of the two different chondroitin sulfate attachment domains (α and β). In this study, genomic DNA analysis has revealed that these domains are encoded by two large exons, exon VII (2880 base pairs) and exon VIII (5229 base pairs). The splice sites of these two exons were consistent with the occurrence of alternative splicing without frameshift. Furthermore, the mouse PG-M gene was shown to have four distinct polyadenylation signals and three candidates for the transcription initiation site as well. These genomic structural variations may contribute to the multiplicity of PG-M transcripts. Northern hybridization analysis showed that at least three different transcripts were generated by different usage of the distinct polyadenylation signals.
Footnotes
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↵* This work was supported in part by grants-in-aid from the Ministry of Education, Culture, and Science of Japan, by special coordination funds for promoting science and technology from the Science and Technology Agency, and by a special research fund from Seikagaku Corp. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The nucleotide sequences reported in this paper have been submitted to the GSDB/DDBJ/EMBL/NCBI data bases with accession numbers D45888 (5′-promoter region of the mouse gene for the PG-M core protein (800 bp)) and D45889 (3′-noncoding region of the mouse gene for the PG-M core protein (2201 bp)).
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↵1 The abbreviations used are:
- kb
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kilobase pair(s)
- bp
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base pair(s)
- PCR
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polymerase chain reaction
- CS
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chondroitin sulfate
- 5′-RACE
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rapid amplification of cDNA 5′-ends.
- © 1995 by The American Society for Biochemistry and Molecular Biology, Inc.
