gp180, a Host Cell Glycoprotein That Binds Duck Hepatitis B Virus Particles, Is Encoded by a Member of the Carboxypeptidase Gene Family (*)
- Kazuyuki Kuroki(1)(§),
- Frank Eng(2),
- Takashi Ishikawa(2),
- Christoph Turck(3)(¶),
- Fumio Harada(1) and
- Don Ganem(2)(3)(¶)
- From the (1) Department of Biophysics, Cancer Research Institute, Kanazawa University, 13-1 Takaramachi, Kanazawa, Ishikawa 920 Japan and Howard Hughes Medical Institute, Departments of
- (2)Microbiology and
- (3)Medicine, University of California, San Francisco, California 94143-0502
- § Supported by a grant-in-aid for scientific research from the Ministry of Education and Culture of Japan and by the Hokkoku Foundation for Cancer Research. To whom correspondence should be addressed. Tel.: 81-762-62-8151; Fax: 81-762-22-5831.
Abstract
Duck hepatitis B virus particles bearing the L and S envelope proteins bind a cellular glycoprotein of 180 kDa (gp180) with high affinity and specificity. Binding is mediated by the pre-S region of the L protein and is blocked by neutralizing but not by non-neutralizing monoclonal antibodies to the virus. These and other properties have suggested that gp180 may be a component of the viral entry machinery. Here we report the purification of gp180 from duck liver and the isolation and characterization of cDNA encoding it. DNA sequence analysis of this cDNA indicates that gp180 is a novel member of the basic carboxypeptidase gene family.
Footnotes
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↵¶ Investigator of the Howard Hughes Medical Institute.
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↵* This work was supported in part by Grant AI 31973 from the National Institutes of Health. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBank
/EMBL Data Bank with accession number(s) U25126.
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↵1 The abbreviations used are:
- DHBV
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duck hepatitis B virus
- kb
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kilobase(s).
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↵2K. Kuroki, unpublished observation.
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↵3F. Eng, unpublished observations.
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↵4K. Kuroki, T. Ishikawa, and D. Ganem, unpublished observations.
