Syntaxin 1 (HPC-1) Is Associated with Chromaffin Granules (*)
- Mitsuo Tagaya(§),
- Shuichi Toyonaga,
- Masami Takahashi(1),
- Akitsugu Yamamoto(2),
- Tomonori Fujiwara(3),
- Kimio Akagawa(3),
- Yoshinori Moriyama(4) and
- Shoji Mizushima
- From the (1)School of Life Science, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-03, Japan, the
- Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo 194, Japan, the
- (2)Department of Physiology, Kansai Medical University, Moriguchi, Osaka 570, Japan, the
- (3)Department of Physiology, School of Medicine, Kyorin University, Mitaka, Tokyo 181, Japan, and the
- (4)Marine Biological Laboratory, Graduate School of Gene Sciences, Faculty of Sciences, Hiroshima University, Mukaishima, Hiroshima 722, Japan
- § To whom all correspondence should be addressed: School of Life Science, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-03, Japan. Fax: 81-426-76-8866.
Abstract
Syntaxin 1 (HPC-1), a component of the receptor for SNAPs (soluble N-ethylmaleimide-sensitive factor attachment proteins), has been implicated in the docking and fusion of synaptic vesicles with the plasma membrane. It was reported that syntaxin 1 in rat brain and chromaffin cells (PC12) is exclusively located on the plasma membrane (Bennett, M. K., Calakos, N., and Scheller, R. H.(1992) Science 257, 255-259; Söllner, T., Bennett, M. K., [Medline] Whiteheart, S. W., Scheller, R. H., and Rothman, J. E.(1993) Cell 75, 409-418). By means of biochemical and morphological analyses, we now show that syntaxin 1 is associated with chromaffin granules in the adrenal medulla. This finding raises the possibility that syntaxin 1 in chromaffin cells is a component of vesicle-SNAP receptor as well as one of target-SNAP receptor on the plasma membrane.
Footnotes
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↵* This work was supported in part by grants-in-aid for scientific research from the Ministry of Education, Science and Culture of Japan and the Ciba-Geigy Foundation (Japan) for the Promotion of Science. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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↵1 The abbreviations used are:
- SNAP-25
-
synaptosome-associated protein of 25 kDa
- NSF
-
N-ethylmaleimide-sensitive factor
- SNAP
-
soluble NSF attachment protein
- SNARE
-
SNAP receptor
- v-SNARE
-
vesicle-SNAP receptor
- t-SNARE
-
target-SNAP receptor
- mAb
-
monoclonal antibody
- MOPS
-
4-morpholinopropanesulfonic acid
- PIPES
-
1,4-piperazinediethanesulfonic acid
- PAGE
-
polyacrylamide gel electrophoresis
- VAMP
-
vesicle-associated membrane protein.
