The Interaction of TFIIIA with Specific RNA-DNA Heteroduplexes (*)
- From the Laboratory of Molecular Embryology, NICHD, National Institutes of Health, Bethesda, Maryland 20892-2710
- § To whom correspondence should be addressed. Tel.: 301-402-2722; Fax: 301-402-1323; awlme{at}helix.nih.gov
Abstract
We examine the association of transcription factor TFIIIA with RNA-DNA heteroduplexes containing sequences from the Xenopus borealis 5 S rRNA gene. Under conditions where TFIIIA selectively binds to 5 S rRNA or the internal control region of the 5 S rRNA gene, no specific association of TFIIIA with DNA-RNA heteroduplexes containing either strand of 5 S DNA could be detected. We discuss our results with respect to specific models of TFIIIA recognition of the internal control region and of DNA-RNA hybrids by zinc finger proteins.
Footnotes
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↵* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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↵1 The abbreviations used are:
- ICR
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internal control region
- bp
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base pair(s).
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- Received July 10, 1995.
- Revision received August 1, 1995.
- © 1995 by The American Society for Biochemistry and Molecular Biology, Inc.
