Molecular Cloning, Structure, and Expression of Mouse Estrogen-responsive Finger Protein Efp
CO-LOCALIZATION WITH ESTROGEN RECEPTOR mRNA IN TARGET ORGANS (*)
- From the (1)Department of Biochemistry, Saitama Medical School, 38 Moro-Hongo, Moroyama-machi, Iruma-gun, Saitama 350-04, Japan and the
- (2) Department of Biological Science and Technology, Faculty of Engineering, University of Tokushima, 2-1 Minami-Jyosanjima-machi, Tokushima 770, Japan
- §To whom reprint requests should be addressed: Dept. of Biochemistry, Saitama Medical School, 38 Moro-Hongo, Moroyama-machi, Iruma-gun, Saitama 350-04, Japan. Tel: 81-492-76-1490; Fax: 81-492-94-9751.
Abstract
We have previously identified a human estrogen-responsive gene, efp (estrogen-responsive finger protein), which encodes a putative transcription regulator (Inoue, S., Orimo, A., Hosoi, T., Kondo, S., Toyoshima, H., Kondo, T., Ikegami, A., Ouchi, Y., Orimo, H., and Muramatsu, M.(1993) Proc. Natl. Acad. Sci. U. S. A. 90, 11117-11121). Here, we report isolation of mouse Efp cDNA and its structure containing three cysteine-rich domains (RING finger and B1 and B2 boxes), a coiled-coil domain, and a C-terminal domain. High levels of Efp mRNA were detected in uterus, ovary, and placenta by RNase protection assay. By in situ hybridization histochemistry the transcripts of efp were also detected in uterus, mammary gland, ovary, and brain, and the co-localization of Efp and estrogen receptor mRNA was particularly demonstrated in these female organs. Moreover, the level of Efp mRNA in uterus and brain, which are known as target organs for estrogen, was up-regulated in vivo by 17β-estradiol. Furthermore, both the Efp and estrogen receptor mRNA were stained in the brain vesicles of 11.5-day embryos by whole mount in situ hybridization. These findings raise the possibility that efp is an estrogen-responsive gene that mediates estrogen action in various target organs.
Footnotes
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↵* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBank™/EMBL Data Bank with accession number(s) D63902[GenBank].
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↵1 The abbreviations used are:
- ER
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estrogen receptor
- ERE
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estrogen-responsive element(s)
- bp
-
base pair(s)
- PML
-
promyelocytic leukemia.
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- Received May 15, 1995.
- Revision received July 5, 1995.
- © 1995 by The American Society for Biochemistry and Molecular Biology, Inc.
