Activation and Translocation of c-Src to the Cytoskeleton by Both Platelet-derived Growth Factor and Epidermal Growth Factor

doi:10.1074/jbc.270.5.2264

Activation and Translocation of c-Src to the Cytoskeleton by Both Platelet-derived Growth Factor and Epidermal Growth Factor (*)

From the Laboratory of Medical Enzymology, Department of Hematology, University Hospital Utrecht, NL-3508 GA Utrecht, The Netherlands

^§ To whom correspondence should be addressed:
Dept. of Hematology, Laboratory of Medical Enzymology, University Hospital Utrecht, P. O. Box 85500, NL-3508 GA Utrecht, The Netherlands.
Tel.: 31-30-507602; Fax: 31-30-511893.

Abstract

We have examined the subcellular distribution and catalytic activity of c-Src tyrosine kinase after stimulation of A172 glioblastoma cells with peptide growth factors. Treatment of resting cells with platelet-derived growth factor resulted in an increase (3.5-fold) in the amount of c-Src protein associated with the cytoskeleton. In addition, an increase in specific c-Src kinase activity was observed in the cytoskeleton as well as in the cytosol and the membrane fraction. Similar effects on both c-Src redistribution and activity were seen after stimulation with epidermal growth factor. These data show that, like other signal transducing components, c-Src also becomes activated and associated to the cytoskeleton in response to growth factor stimulation.

Footnotes

↵* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
↵¹ The abbreviations used are:

PDGF

platelet-derived growth factor

EGF

epidermal growth factor

SH

Src homology

mAb

monoclonal antibody

PAGE

polyacrylamide gel electrophoresis

PBS

phosphate-buffered saline

BSA

bovine serum albumin

PVDF

polyvinylidene difluoride

RIPA

radioimmune precipitation buffer

PIPES

1,4-piperazinediethanesulfonic acid.
↵²P. A. Oude Weernink and G. Rijksen, unpublished observation.
- Received November 17, 1994.