cDNA Cloning and Characterization of a Cek7 Receptor Protein-tyrosine Kinase Ligand That Is Identical to the Ligand (ELF-1) for the Mek-4 and Sek Receptor Protein-tyrosine Kinases (*)
- Haining Shao,
- Liandi Lou,
- Akhilesh Pandey,
- Michael F. Verderame(1),
- Doyle A. Siever(1) and
- Vishva M. Dixit(§)
- From the Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109
- Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033
- § Established Investigator of the American Heart Association. To whom correspondence should be addressed: Dept. of Pathology, University of Michigan Medical School, 1301 Catherine St., Ann Arbor, MI 48109-0602. Tel.: 313-747-0264; Fax: 313-764-4308. vishva_dixit{at}mailqm.pds.med.umich.edu.
Abstract
We have isolated a murine cDNA encoding a ligand for the Cek7 receptor protein-tyrosine kinase (RPTK), a member of the Eph/Eck RPTK subfamily. Sequence analysis predicts an open reading frame of 209 amino acids with a predicted molecular mass of 24 kDa. The Cek7 ligand shows a 48% sequence identity at the protein level to B61, a ligand for the related Eck RPTK, 30% to the Cek5 ligand, 59% to the recently cloned Ehk1-L, and identity to ELF-1, a recently described ligand for the Mek4 and Sek RPTKs. The expressed Cek7 ligand is functionally active as it induces autophosphorylation of the Cek7 RPTK.
Footnotes
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↵* This work was supported by National Institutes of Health Grants DK39255 (to V. M. D.), CA52791 (to M. F. V.), and CA60395 (to D. A. S.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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↵1 The abbreviations used are:
- RPTK
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receptor protein-tyrosine kinase
- GPI
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glycosylphosphatidylinositol
- CHO
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Chinese hamster ovary
- PCR
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polymerase chain reaction
- PBS
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phosphate-buffered saline.
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↵2Shao, H., Pandey, A., O'Shea, K. S., Seldin, M., and Dixit, V. M.(1995) J. Biol. Chem.270, in press.
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- Received December 15, 1994.
- Revision received January 5, 1995.
- © 1995 by The American Society for Biochemistry and Molecular Biology, Inc.
