ERGIC-53, a Membrane Protein of the Endoplasmic Reticulum-Golgi Intermediate Compartment, Is Identical to MR60, an Intracellular Mannose-specific Lectin of Myelomonocytic Cells (*)
- Chantal Arar(1)(§),
- Valérie Carpentier(1)(§),
- Jean-Pierre Le Caer(2),
- Michel Monsigny(1),
- Alain Legrand(1) and
- Annie-Claude Roche(1)(¶)
- From the (1)Laboratoire de Biochimie des Glycoconjugués et Lectines Endogènes, Université d'Orléans et Centre de Biophysique Moléculaire, CNRS, Bâtiment B, rue Charles Sadron, 45071 Orléans, Cedex 02, France and the
- (2)Institut Alfred H. Fessard, CNRS, 91198 Gif-sur-Yvette, Cedex, France
- ¶ Directeur de Recherche INSERM. To whom correspondence should be addressed. Tel.: 33-38-51-55-37; Fax: 33-38-69-00-94; roche{at}cnrs-orleans.fr.
Abstract
A mannose-specific membrane lectin (MR60) isolated from human myelomonocytic HL60 cells by affinity chromatography is expressed in intracellular organelles of immature monocytes (Pimpaneau, V., Midoux, P., Monsigny, M., and Roche, A. C. (1991) Carbohydr. Res. 213, 95-108). It is not present at the cell surface and is immunochemically and structurally distinct from the Mr 175,000 mannose receptor of mature macrophages. MR60 cDNA was isolated and characterized; on the basis of its sequence, MR60 is not related to any known mammalian lectins. Surprisingly, MR60 was found to be identical to ERGIC-53 (Schindler, R., Itin, C., Zerial, M., Lottspeich, F., and Hauri, H. P.(1993) Eur. J. Cell Biol. 61, 1-9), a type I integral membrane protein, defined as a marker of the intermediate compartment that recycles between the Golgi apparatus and endoplasmic reticulum; MR60/ERGIC-53 shares with VIP-36 significant homologies with leguminous plant lectins (Fiedler, K., and Simmons, K.(1994) Cell 77, 625-626). We extend these findings in evidencing a structural homology between MR60/ERGIC-53 and mammalian galectins (soluble β galactose binding proteins). MR60/ERGIC-53 is the first lectin characterized as an endoplasmic reticulum-Golgi protein. Accordingly, this intracellular mannose binding protein could be involved in the traffic of glycoproteins between endoplasmic reticulum and the Golgi apparatus.
Footnotes
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↵§ Received a fellowship from the Ministère de la Recherche et de la Technologie.
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↵* This work was supported in part by Association de Recherche sur le Cancer Grants ARC 6132 (to A.-C. R.) and ARC 6231 (to A. L.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBank(TM)/EMBL Data Bank with accession number(s) U09716[GenBank].
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↵1 The abbreviation used is:
- PSL
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pea seed lectin.
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- Received November 2, 1994.
- Revision received December 14, 1994.
- © 1995 by The American Society for Biochemistry and Molecular Biology, Inc.
