Expression of PG-M(V3), an Alternatively Spliced Form of PG-M without a Chondroitin Sulfate Attachment Region in Mouse and Human Tissues

doi:10.1074/jbc.270.8.3914

Expression of PG-M(V3), an Alternatively Spliced Form of PG-M without a Chondroitin Sulfate Attachment Region in Mouse and Human Tissues (*)

From the Institute for Molecular Science of Medicine, Aichi Medical University, Nagakute, Aichi 480-11, Japan

^§ To whom correspondence should be addressed. Tel.: 052-264-4811 (ext. 2088); Fax: 0561-63-3532.

Abstract

We showed previously that the alternative splicing of chondroitin sulfate attachment domains (CS α and CS β) yielded multiforms of the PG-M core protein in mouse. A transcript encoding a new short form of the core protein PG-M(V3) was found in various mouse tissues using polymerase chain reaction. DNA sequences of the polymerase chain reaction products suggested that PG-M(V3) had no chondroitin sulfate attachment domain. PG-M(V3) was also detected in various human tissues. The presence of a transcript for PG-M(V3) was further supported by Northern blot analysis. Southern blot analysis confirmed that multiforms of the PG-M core protein, including PG-M(V3), were derived from a single genomic locus by an alternative splicing mechanism. Because PG-M(V3) has no chondroitin sulfate attachment region, which is the most distinctive portion of a proteoglycan molecule, this form may have a unique function.

Footnotes

↵* This work was supported in part by Special Coordination Funds of the Science and Technology Agency of the Japanese Government, a grant-in-aid from the Ministry of Education, Culture, and Science of the Japanese Government, and a special research fund from the Seikagaku Corporation. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank(TM)/EMBL Data Bank with accession number(s) D32040 [GenBank]and D32039 [GenBank]for mouse and human PG-M(V3), respectively.
↵¹ The abbreviations used are:

EGF

epidermal growth factor

CRP

complement regulatory protein

CS

chondroitin sulfate

GHAP

glial hyaluronan-binding protein

PCR

polymerase chain reaction

kb

kilobase pair(s)

bp

base pair(s).
- Received September 23, 1994.
- Revision received November 30, 1994.