DNA Repair Protein XPA Binds Replication Protein A (RPA) (*)
- Toshiro Matsuda(1),
- Masafumi Saijo(1),
- Isao Kuraoka(1),
- Takehiro Kobayashi(1),
- Yoshimichi Nakatsu(1),
- Akira Nagai(1),
- Takashi Enjoji(1),
- Chikahide Masutani(2),
- Kaoru Sugasawa(2),
- Fumio Hanaoka(2),
- Akira Yasui(3) and
- Kiyoji Tanaka(1)(§)
- From the (1)Institute for Molecular and Cellular Biology, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565, the
- (2)Institute of Physical and Chemical Research (RIKEN), 2-1 Hirosawa, Wako-shi, Saitama 351-01, and the
- (3)Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryomachi, Aoba-ku, Sendai 980, Japan
- § To whom correspondence should be addressed. Tel.: 81-6-877-5238; Fax: 81-6-877-9136.
Abstract
XPA is a zinc finger DNA-binding protein, which is missing or altered in group A xeroderma pigmentosum cells and known to be involved in the damage-recognition step of the nucleotide excision repair (NER) processes. Using the yeast two-hybrid system to search for proteins that interact with XPA, we obtained the 34-kDa subunit of replication protein A (RPA, also known as HSSB and RFA). RPA is a stable complex of three polypeptides of 70, 34, 11 kDa and has been shown to be essential in the early steps of NER as well as in replication and recombination. We also demonstrate here that the RPA complex associates with XPA. These results suggest that RPA may cooperate with XPA in the early steps of the NER processes.
Footnotes
-
↵* This work was supported by a grant-in-aid for scientific research on priority areas from the Ministry of Education, Science and Culture of Japan, and grants from the Mitsubishi Foundation, Uehara Memorial Foundation, and Human Frontier Science Program. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
-
↵1 The abbreviations used are:
- XP
-
xeroderma pigmentosum
- RPA
-
replication protein A
- NER
-
nucleotide excision repair
- ERCC
-
excision repair cross-complementing rodent repair deficiency
- PCR
-
polymerase chain reaction
- PMSF
-
phenylmethylsulfonyl fluoride
- PAGE
-
polyacrylamide gel electrophoresis
- GST
-
glutathione S-transferase.
-
↵2I. Kuraoka, unpublished results.
-
- Received October 31, 1994.
- Revision received December 10, 1994.
- © 1995 by The American Society for Biochemistry and Molecular Biology, Inc.
