Molecular Cloning of F4/80, a Murine Macrophage-restricted Cell Surface Glycoprotein with Homology to the G-protein-linked Transmembrane 7 Hormone Receptor Family (*)
- Andrew J. McKnight(§),
- Alison J. Macfarlane,
- Pietro Dri(¶),
- Laurence Turley,
- Anthony C. Willis(1) and
- Siamon Gordon
- From the Sir William Dunn School of Pathology and the
- Medical Research Council Immunochemistry Unit, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom
- § To whom correspondence should be addressed.
Abstract
F4/80 is a monoclonal antibody that recognizes a murine macrophage-restricted cell surface glycoprotein and has been extensively
used to characterize macrophage populations in a wide range of immunological studies. Apart from the tightly regulated pattern
of expression of the F4/80 antigen, little is known about its possible role in macrophage differentiation and function. We
have sought to characterize the molecule at the molecular level, through the isolation of cDNA clones, and now describe the
sequence of the F4/80 protein. The primary amino acid sequence demonstrates homology to two protein superfamilies. The NH
-terminal region consists of seven epidermal growth factor-like domains, separated by approximately 300 amino acids from a
COOH-terminal region that shows homology to members of the seven transmembrane-spanning family of hormone receptors. The potential
role of these distinct domains is discussed with respect to the possible function of the F4/80 molecule.
Footnotes
-
↵* This work was supported by the Medical Research Council, United Kingdom. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBank(™)/EMBL Data Bank with accession number(s) X93328.
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↵1 The abbreviations used are:
- mAb
-
monoclonal antibody
- PCR
-
polymerase chain reaction
- bp
-
base pair(s)
- EGF
-
epidermal growth factor.
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↵2A. J. McKnight, A. J. MacFarlane, and S. Gordon, unpublished results.
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- Received July 28, 1995.
- Revision received September 26, 1995.
- © 1996 by The American Society for Biochemistry and Molecular Biology, Inc.
