Nuclear Matrix Interactions within the Sperm Genome

doi:10.1074/jbc.271.20.11619

Nuclear Matrix Interactions within the Sperm Genome (*)

From the Department of Obstetrics and Gynecology, Center for Molecular Medicine and Genetics, and C. S. Mott Center for Human Growth and Development, Wayne State University School of Medicine, Detroit, Michigan 48102

Abstract

Analysis of the haploid-expressed human PRM1 PRM2 TNP2 genic domain has revealed two regions of attachment to the sperm nuclear matrix. These sperm nuclear matrix attachment regions delimit the DNase I-sensitive domain of this haploid-expressed locus. The domain is intermediately associated with but not attached to the nuclear matrix. DNase I-sensitive genes within the mature sperm nucleus, such as protamine 1, protamine 2, transition protein 2, α-globin, and β-actin, display this intermediate affinity for the sperm nuclear matrix. This may denote their role in templating the male genome prior to fertilization, thus ensuring the formation of a viable male pronucleus during early embryonic development.

Footnotes

↵^§ Supported in part by the Dean's postdoctoral recruitment fellowship.
↵* This work was supported by Grant 1R01HD2850401A1 (to S. A. K.) from the National Institute of Child Health and Development and Grant EDUD-US93015 from SUN microsystems. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore by hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank(™)/EMBL Data Bank with accession number(s) U15422.
↵¹ The abbreviations used are:

kb

kilobase(s)

SMAR

sperm nuclear matrix attachment region

MAR

somatic nuclear matrix attachment region

PCR

polymerase chain reaction

bp

base pair(s).
↵²Primer sequences, PCR conditions, and the PRM1 PRM2 TNP2 domain sequence will be made available at the internet address “http://compbio.med.wayne.edu/”.
↵³J. A. Kramer and S. A. Krawetz, unpublished observations.
- Received March 1, 1996.
- Revision received March 25, 1996.