An E-box region within the prostaglandin endoperoxide synthase-2 (PGS-2) promoter is required for transcription in rat ovarian granulosa cells.

The prostaglandin endoperoxide synthase-2 (PGS-2) gene encodes an isoform of prostaglandin synthase that is transiently induced by protein kinase A (luteinizing hormone/cAMP) and protein kinase C (gonadotropin-releasing hormone) agonists in granulosa cells of ovulating follicles. The promoter of the rat PGS-2 gene contains a CAAT enhancer-binding protein consensus site (CAAT box) which can confer hormone inducibility to a PGS-2·CAT reporter gene, as well as a putative E-box region. To determine if the E-box region was involved in hormone induced trans-activation of the rat PGS-2 gene, constructs with the CAAT box and E-box regions (−192 PGS-2·CAT), only the putative E-box (−110 PGS-2·CAT), or neither region (−52 PGS-2·CAT) were transiently transfected into rat granulosa cell cultures. CAT activity was induced in both the −192 and −110 PGS-2·CAT vectors by luteinizing hormone (10-fold) and gonadotropin-releasing hormone (6-fold), whereas CAT activity of the −52 PGS-2·CAT construct did not differ from the promoterless vector (pCAT-Basic). Deletion of 1 base pair from the E-box within the −110 PGS-2·CAT construct, as well as point mutations within the CAAT box, E-box, or both regions of the −192 PGS-2·CAT construct, demonstrated that the E-box is critical for basal transcription, and that regions, in addition to the CAAT box, are involved in hormone induction of the PGS-2 gene. An oligonucleotide spanning the rat PGS-2 E-box bound two specific protein complexes which were supershifted in the presence of antibody specific for the upstream stimulatory factor. Thus, in rat granulosa cells, the PGS-2 E-box region appears to interact with upstream cis-acting elements other than the CAAT box to confer hormonal regulation of the gene. The E-box region of the rat PGS-2 promoter does not contain ATF/CRE activity found in the human and mouse PGS-2 promoters, but is critical for basal transcription of the PGS-2 gene in rat granulosa cells and binds the upstream stimulatory factor (as do E-box regions of other genes regulated in the ovary).

Prostaglandin endoperoxide synthase (PGS) 1 catalyzes the rate-limiting step of prostaglandin biosynthesis (1). The synthase is a homodimer with two subunits and has two catalytic properties, a cyclooxygenase activity that is required for the conversion of arachidonic acid to prostaglandin G 2 and a peroxidase activity that reduces prostaglandin G 2 to prostaglandin H 2 (1). Prostaglandins are associated with inflammatory responses, wound healing, bone development, glomerular filtration and water balance, and hemostasis (for a review, see Ref. 2). Prostaglandins have also been shown to regulate a variety of reproductive functions, including ovulation (3) and parturition (4). For example, ovulation is inhibited by aspirin, indomethacin, and specific inhibitors of PGS, in mice (5), rats (6 -8), rabbits (9,10), and pigs (11). Most recently, targeted deletion of the PGS-2 gene has been shown to prevent ovulation in mice (12), whereas targeted disruption of the PGS-1 gene did not alter ovarian function, but did compromise parturition (13).
Two isoforms of the PGS enzyme are present in the rat ovary (14,15). Each enzyme is the product of a distinct gene as evidenced by the cloning of cDNAs for PGS-1 (16 -18) and PGS-2 (19 -21). PGS-1 is encoded by a 2.8-kilobase pair RNA transcript and the enzyme is ubiquitously expressed in theca cells of the ovarian follicle (15), as well as other tissues (22). PGS-2 is encoded by a 4.4-kilobase pair RNA transcript and is rapidly induced in ovarian granulosa cells by luteinizing hormone (LH), follicle-stimulating hormone (FSH), forskolin, gonadotropin-releasing hormone (GnRH), and epidermal growth factor (14,15,23). The presence of PGS-2 mRNA in granulosa cells corresponds to the specific immunological localization of this protein in granulosa cells, not theca cells (14,15,24). PGS-2 mRNA is rapidly and transiently induced in the ovary (25) and other tissues by activators of several signaling pathways including protein kinase A, protein kinase C, and tyrosine kinase pathways (22,23,26,27).
The functional region of the mouse PGS-2 promoter contains NFB, C/EBP, and an overlapping ATF/CRE (5Ј-CGTCA-3Ј) and E-box (5Ј-CACGTG-3Ј) transcription factor recognition sequences (35)(36)(37). Mutational analysis of the NFB and C/EBP elements demonstrated that both these sites were important for tumor necrosis factor-␣ induction of PGS-2⅐luciferase constructs transfected into MC3T3-E1 osteoblastic cells (35). Mutational analysis of the ATF/CRE element within the mouse promoter demonstrated that the ATF/CRE element, but not the E-box element, is essential for v-src induction of PGS-2⅐luciferase constructs transfected into NIH 3T3 fibroblast cells (36). Although the E-box was not required for v-src activation of the PGS-2 expression vectors (36), mutations of the E-box reduced basal levels of transcription (36). Electrophoretic mobility shift assays demonstrated that an end-labeled mouse PGS-2 oligonucleotide (Ϫ65/Ϫ39 bp), containing ATF/CRE and E-box elements, bound several fibroblast extract proteins which by competition assays and antibody analyses were identified as CREB, c-Jun, and two E-box-binding proteins (36,37). Furthermore, c-Jun, and not CREB, was shown to be required for v-src induction of PGS-2 promoter constructs in NIH 3T3 fibroblasts (37).
When human PGS-2 promoter reporter vectors were transfected into bovine arterial endothelial cells (33) or human U937 monocytic cells (34), various regions (NFB, CAAT box (C/ EBP), and ATF/CRE) were shown to be important for the expression of transgenes dependent upon the cell type. Although deletion to Ϫ52 disrupted the putative human PGS-2 E-box and abolished functional activity of the human PGS-2 transgenes, specific functional analysis of this element in the context of other regions has not yet been reported. In addition, the human PGS-2 E-box element is different by one base pair from the E-box element present in the rat and mouse PGS-2 promoters (see Table I). Therefore, the human E-box element might bind different E-box binding proteins, than those which bind the rat or mouse PGS-2 E-box element.
In the 5Ј-flanking region of the rat PGS-2 gene, a CAAT box region (Ϫ140/Ϫ132) has been identified and shown to confer cAMP-responsiveness when ligated to a minimal Ϫ52/32 PGS-2⅐CAT reporter construct (38). Mutation of this CAAT box region decreased cAMP inducibility of the fusion construct in rat ovarian granulosa cells by 50%. The CAAT box binds to C/EBP␣ and C/EBP␤ proteins present in nuclear extracts of rat granulosa cells (38). Furthermore, LH induces C/EBP␤ mRNA and decreases C/EBP␣ mRNA coordinately with the induction of the PGS-2 gene (38). However, because the PGS-2⅐CAT fusion vectors used in these experiments disrupted what has recently been characterized as a CRE/ATF and E-box regulatory region in the mouse promoter, additional analyses were required to determine the specific role of each region for transactivation of the rat PGS-2 promoter in ovarian granulosa cells (38).
Based on the evidence that multiple factors bind the ATF/ CRE, E-box and CAAT elements of the PGS-2 gene, and that these regions confer trans-activation in an agonist, cell-type, or species promoter-specific manner, we have designed experiments to determine if the ATF/CRE or E-box region of the rat PGS-2 promoter might also be functionally relevant for transactivation of the rat PGS-2 gene by LH and GnRH in granulosa cells. Transient transfection of reporter constructs in rat granulosa cells demonstrated that the E-box element is critical for basal activation of the PGS-2 gene in granulosa cells and that one transcription factor which binds to this region is an upstream stimulatory factor (USF). In addition, when specific mutants within the CAAT box and E-box regions were made in context of the intact promoter and ligated to a pCAT⅐Basic reporter construct, the consensus C/EBP site was not essential for activation of PGS-2 reporter constructs by either LH or GnRH, suggesting that the E-box region interacts with other cis-acting elements in the proximal (Ϫ110/Ϫ52) region of the rat PGS-2 promoter.

EXPERIMENTAL PROCEDURES
Animals-Immature female rats (day 23 of age; weight, 55-60 g) were injected with 17␤-estradiol (1.5 mg/day for 3 days) beginning on day 24 of age (25,39,40). Hypophysectomized immature female rats (day 26 of age) were obtained from Harlan (Indianapolis, IN) 1 day after surgery. Animals were primed with 17␤-estradiol (E; 1.5 mg/day subcutaneously for 3 days) and FSH (F; 1.0 g subcutaneously, twice daily for 2 days) designated HE or HEF, respectively (23). Animals were treated in accordance with the NIH Guide for the Care and Use of FIG. 1. Comparison of the ؊198/32 region of the rat, mouse, and human PGS-2 5-flanking sequences. The E-box region is underlined (31,33,36).
Laboratory Animals. All protocols had the approval of the Institutional Committee on Animal Care and Use, Baylor College of Medicine, Houston, TX.
Granulosa Cell Culture-Ovaries from immature rats primed with 17␤-estradiol were punctured with a 22-gauge needle to isolate granulosa cells. The granulosa cells were pooled, treated with trypsin (20 g/ml) for 1 min, followed by addition of soybean trypsin inhibitor (300 g/ml) and DNase I (160 g/ml) to remove necrotic cells, as described previously (39). Cells were washed twice and then cultured in Dulbecco's modified Eagle's medium:F12 supplemented with ovine FSH (50 ng/ml) and testosterone (10 ng/ml) at 37°C in 95% air and 5% CO 2 . After 48 h, granulosa cells cultured under these conditions acquire a preovulatory phenotype (39,40) and respond to ovulatory doses of either FSH or LH as indicated by the induction of specific enzymes, including PGS-2 (15,25), and the progesterone receptor (41) within 5-7 h.
Transient Transfection Assay-Plasmid DNA was purified from bacteria by alkaline lysis and centrifugation on CsCl gradients (42). The differentiated granulosa cells were transfected with 12.5 g/well of plasmid DNA as described previously (43). Briefly, the plasmid DNA was precipitated by dropwise addition of a 250 mM CaCl and plasmid DNA mixture in an equal volume of 2 ϫ HEPES-buffered saline (280 mM NaCl, 1.5 mM Na 2 HPO 4 , 50 mM HEPES, pH 7.2) and incubated at room temperature for 30 min. After the addition of fresh Dulbecco's modified Eagle's medium:F12 to the differentiated granulosa cells, 300 l of the DNA precipitate were added dropwise to each well. For transfection, the cells were incubated for 4 h at 37°C in 95% air and 5% CO 2 , washed once with Hanks' buffered medium without calcium or magnesium, and treated with either no hormone, LH (500 ng/ml), or GnRH (1 M). After 6 h, the granulosa cells were washed with 2 ml of ice-cold phosphate-buffered saline (PBS, pH 7.4), harvested by gentle scraping with a rubber policeman in 1 ml of TEN (10 mM Tris-Cl (pH 8.0), 1 mM EDTA (pH 8.0), 0.1 M NaCl), and centrifuged (12,000 ϫ g, 4°C) to obtain a cell pellet. The cell pellet was resuspended in 100 l of 0.25 M Tris-HCl, frozen on dry ice-ethanol, thawed, followed by two additional freeze/thaw cycles, and finally clarified by centrifugation at 4°C at 12,000 ϫ g. CAT activity in the lysates (25 g of protein) was measured in the presence of 25 mM acetyl-coenzyme A and 2 l per reaction of [ 14 C]chloramphenicol as described previously (44).
Whole Cell and Nuclear Extracts-Whole cell extracts were prepared from R2C cells as described previously (45). Nuclear extracts of granulosa cells isolated from hormonally primed rats were prepared as described previously (31,46), designated HE and HEF. Nuclear extracts were also prepared from granulosa cells isolated from HEF rats 2 h after an intravenous injection of human chorionic gonadotropin (hCG, 10 IU) designated HEF ϩ hCG (2 h).
Immunoblot Analyses-Proteins of whole cell lysates from granulosa cell cultures were resolved by one-dimensional SDS-polyacrylamide gel electrophoresis (4.5% stacking gel and 10% separating gel) at 60 mA/gel for 1 h. Proteins were transferred to nitrocellulose filters (0.45 M) overnight at 30 V and blocked in 5% milk, PBS (80 mM disodium hydrogen orthophosphate anhydrous, 20 mM sodium dihydrogen orthophosphate, 100 mM NaCl, pH 7.5) with 0.1% Tween-20 (PBS-T) for 1 h, washed in PBS-T for 5 min (three times). The nitrocellulose filter was incubated in a 1:5000 dilution of USF antibody (from Dr. M. Sawadogo, M. D. Anderson Cancer Center, Houston, TX) in PBS-T, 5% milk for 1 h at room temperature. The membrane was washed once for 15 min and three times for 5 min in PBS-T, then incubated in antirabbit immunoglobulin G conjugated to horseradish peroxidase (1:10,000 dilution) for 1 h at room temperature. The filter was washed once for 15 min and three times for 5 min. The immunopositive proteins were detected by enhanced chemiluminescence (ECL), as described by Amersham Corp.
Three additional mutations of the PGS-2 promoter were created using the Ϫ195/32 PGS-2⅐CAT construct as a template, Ϫ195/32 (C/EBP Mt) PGS-2⅐CAT, Ϫ195/32 (E-box Mt) PGS-2⅐CAT and a Ϫ195/32 (Double Mt) PGS-2⅐CAT. The C/EBP Mt was created by substituting four base pairs within the CAAT box that disrupts the binding of C/EBP␣ and ␤ protein. In EMSA, the C/EBP mutant oligonucleotide did not compete for the binding of C/EBPs to a labeled Ϫ195/Ϫ110 DNA fragment (25). The E-box mutant was created with an oligonucleotide containing a two base pair substitution which also fails to compete for the binding of proteins to a labeled wild type Ϫ64/Ϫ41 oligonucleotide (see Fig. 3A, lane 7). Finally, the Double Mt includes these same mutations within both the CAAT box and the E-box.
The mutations (indicated by lowercase) within the Ϫ195 to 32 base pairs of the PGS-2 promoter were created by the polymerase chain reaction (PCR) using mutant oligonucleotides to the CAAT box (5Ј-GGT ATT ATt Ccc aTG GAA GC-3Ј and 5Ј-GCT TCC Atg gGa ATA ATA CC-3Ј) and the E-box (5Ј-AGT CAC GAA GTC ACt TtG AGT CCA-3Ј and 5Ј-AAG TGG ACT CaA aGT GAC TTC GT-3Ј), external primers that would hybridize at each end of the promoter region to be cloned (5Ј-GCC AAG CTT CCT GGC TTC TC-3Ј and 5Ј-TGC TCT AGA GCT ACT CCT GAA GCT CTC CGC TC-3Ј), and Ϫ195/32 PGS-2⅐CAT fusion construct as a template for the E-box and C/EBP mutant oligonucleotides. The Ϫ195/32 (Double Mt) PGS-2⅐CAT was generated with the same external oligonucleotides, the E-box mutant oligonucleotides and the Ϫ195/32 (C/EBP Mt) PGS-2⅐CAT construct as the template. The cloning procedure was as described in PCR protocols (48). The first round of PCR consisted of 30 cycles at 72°C (1 min), 42°C (1 min), and 95°C (1 min). The PCR products were then separated and purified from a 2% agarose gel utilizing NA45 paper (Schleicher & Schuell) and eluted as described previously (49). The second round of PCR utilized 15 cycles of 72°C (1 min), 42°C (1 min), and 95°C (1 min), followed by an additional 15 cycles with an annealing temperature of 50 C. The PCR products were isolated as above, digested with HindIII and XbaI, and ligated into the HindIII and XbaI sites of a digested pCAT⅐Basic vector. All plasmids were sequenced on an automated sequencer according to Taq DyeDeoxy Terminator cycle sequencing for verification of the mutations (University of Texas-Houston, Core Facility).

An E-box cis-Acting DNA Element Is Required for Transcriptional Activation of rPGS-2 Promoter in Rat Ovarian Granulosa Cells-Previous functional analyses of rat PGS-2⅐CAT promoter expression vectors in primary ovarian granulosa cells demonstrated that
Ϫ195 base pairs of the proximal promoter were sufficient to confer LH, FSH, and forskolin inducibility to the PGS-2⅐CAT vectors (38). A CAAT box element within this region (Ϫ195/Ϫ110) was shown to bind C/EBP proteins, specifically C/EBP␣ and C/EBP␤, and to confer cAMP (FSH, LH, and forskolin) inducibility if ligated to a minimally active Ϫ52/32 PGS-2⅐CAT construct (38). Transfection of the (Ϫ192/ Ϫ110)(Ϫ52/32) PGS-2⅐CAT fusion construct exhibited a decrease in basal transcription (38). Based on recent reports, this construct disrupted the ATF/CRE and/or E-box region (Ϫ67/ Ϫ39) (33,36).
To determine the functional activity of the Ϫ110/Ϫ53 region of the rat PGS-2 promoter, we have made additional constructs by ligating a Ϫ110/Ϫ53 oligonucleotide onto the Ϫ52 PGS-2⅐CAT fusion construct. A mutant construct (Ϫ110 mt PGS-2⅐CAT) with a 1-base pair deletion within the E-box region (CACGTG to CA_GTG) was also created. The Ϫ195, Ϫ110, Ϫ110 Mt, and Ϫ52 PGS-2⅐CAT constructs were transiently transfected into differentiated granulosa cells and exposed to LH ( Fig. 2A). All values were measured within the linear range of the assay and then adjusted to the relative CAT activity for 25 g of protein extract. The vector pCAT⅐Control (Promega, Madison, WI), which contains the simian virus 40 promoter and enhancer, was utilized in each transfection experiment as a control for transfection efficiency. The pCAT⅐Control vector routinely displayed a relative CAT activity of 80 Ϯ 10 indicating that transfection efficiency was reproducible among all experiments. The Ϫ195 PGS-2⅐CAT vector exhibited significantly higher relative basal activity than pCAT⅐Basic, which lacks a promoter. This was reduced 33% with the Ϫ110 PGS-2⅐CAT construct, which lacks the CAAT box element (Ϫ142/ Ϫ139). Basal activity decreased an additional 40% by mutation of the E-box. The relative CAT activity stimulated by LH was 88.7 Ϯ 18 and 30 Ϯ 10 relative activity for the Ϫ195 and Ϫ110 PGS-2⅐CAT constructs, respectively. However, the relative CAT activity of the Ϫ110 Mt vector induced by LH, was not different from the Ϫ52 PGS-2⅐CAT construct, 5.5 Ϯ 3 and 3.6 Ϯ 0.2, respectively. Thus, by disrupting the E-box region, basal, as well as LH-induced, trans-activation of the Ϫ110 PGS-2⅐CAT reporter construct was reduced in differentiated granulosa cell cultures. Collectively, these data indicate that the E-box region is a functional cis-acting DNA element within the rat PGS-2 promoter that is essential for transcriptional activation of the PGS-2 gene in granulosa cells.
Previous studies have demonstrated that two distinct signaling pathways induce PGS-2 mRNA and protein in preovulatory granulosa cells (23,26,27). One pathway involves LH activation of protein kinase A; the other involves GnRH activation of protein kinase C (27). In order to determine if the regions of the rat PGS-2 promoter, that are activated by LH, are also activated by GnRH, the Ϫ195, Ϫ110, Ϫ110 Mt, and Ϫ52 PGS⅐CAT constructs were transiently transfected into differentiated granulosa cells and exposed to GnRH (1 M ; Fig. 2B). The relative basal level of CAT activity measured in granulosa cells transfected with pCAT⅐Basic was minimal and was not altered by addition of GnRH to the cultures. Addition of GnRH increased CAT activity produced by the Ϫ195 PGS-2 construct 3-fold, a level of CAT activity approximately one-third of that induced by LH. Relative CAT activity of the Ϫ110 PGS-2⅐CAT construct induced by GnRH did not significantly decrease compared to the Ϫ195 PGS-2 CAT construct, while the fold induction of Ϫ110 PGS-2⅐CAT by GnRH increased from 3-to 6-fold due to a decrease in the basal activity of the Ϫ110 PGS-2⅐CAT construct. Disruption of the E-box cis-element within constructs, Ϫ110 Mt and Ϫ52 PGS-2⅐CAT, decreased GnRH induction 50%, when compared to the nonmutated Ϫ110 PGS-2⅐CAT promoter construct. These data support the obligatory role of the E-box element for trans-activation of the PGS-2 gene by these two distinct signaling pathways in granulosa cells.
Binding of Protein Complexes to the E-box Is Not Determined by the Stage of Follicular Development-As mentioned above, the mouse PGS-2 promoter contains an ATF/CRE and E-box region that is activated in NIH 3T3 cells by cotransfection with a v-src expression plasmid (36). Mutation of these elements within the promoter demonstrated that the ATF/CRE element, but not the E-box, is important for v-src trans-activation of the mouse PGS-2 promoter in NIH 3T3 cells (36). Although the E-box cis-element did not appear to be involved in the v-src induction of the PGS-2 gene in NIH 3T3 cells, a decrease in basal transcription was seen when the E-box element was mutated (36).
To determine if similar, or the same proteins, might bind to the E-box region (Ϫ67/Ϫ41) of the rat PGS-2 promoter, nuclear extracts were prepared from preovulatory granulosa cells induced with an ovulatory dose of hCG for 2 h; a treatment known to induce expression of the endogenous PGS-2 gene (25). When nuclear extract protein (1 g) was incubated with an end-labeled Ϫ67/Ϫ41 oligonucleotide containing the rat PGS-2 promoter E-box element, two protein-DNA complexes were formed (designated I and II; Fig. 3A). The binding reaction was specific as demonstrated by the ability of 10-, 50-, and 100-fold molar excess of unlabeled Ϫ67/Ϫ41 oligonucleotide to inhibit complex formation (Fig. 3A, lanes 3-5). Additionally, 100-fold excess of an oligonucleotide 5Ј of the E-box region (Ϫ110/Ϫ54) was unable to block the formation of the two protein-DNA complexes (Fig. 3A, lane 6). The oligonucleotide mutated within the E-box region (Ϫ67/Ϫ41 Mt; CACGTG to CACtTt) also did not prevent complex formation (Fig. 3A, lane 7). These results indicated that granulosa cell nuclei contained protein(s) capable of binding specifically to an E-box element.
To determine if the E-box binding protein(s) present in the nuclear extract of granulosa cells was hormonally regulated during follicular development, the end-labeled Ϫ67/Ϫ41 oligonucleotide was incubated with nuclear extracts prepared from granulosa cells at different stages of follicular development. Nuclear extracts were collected from granulosa cells of preantral/small antral follicles (HE), preovulatory follicles (HEF), and from preovulatory granulosa cells that had received an ovulatory dose of hCG (HEF/hCG) (2 h). Both protein-DNA complexes previously observed were present at all stages of follicular development (Fig. 3B, lanes 2-4). Additionally, similar protein-DNA complexes were formed with whole cell extracts isolated from R2C cells, a rat Leydig cell line (Fig. 3B,  lane 5).
Role of the CAAT Box and E-box cis-Elements in trans-Activation of the PGS-2 Gene-When isolated from each other, the C/EBP (38) and E-box cis-elements appear capable of conferring functional activation of the PGS-2 promoter by binding specific factors. Therefore, it was essential to determine the requirement of each site within the context of an intact promoter. The mutations within the promoter were generated to disrupt the DNA elements binding C/EBP␣ and C/EBP␤ (38) constructs, in addition to the wild type Ϫ195 PGS-2⅐CAT vector, were transiently transfected into differentiated granulosa cells as described previously (Fig. 4A). The relative basal CAT activity in cells transfected with the Ϫ195 PGS-2 construct was similar to that obtained for the C/EBP Mt construct (11.5 Ϯ 5 versus 12.3 Ϯ 1.7, respectively). Likewise, the relative CAT activity (91 Ϯ 26 versus 124 Ϯ 47), as well as fold increase (10 Ϯ 3 versus 10 Ϯ 3-fold), induced by LH were similar for the Ϫ195 PGS-2⅐CAT and the C/EBP Mt, respectively. Thus, in the context of the intact promoter, the consensus CAAT box is not required for LH induction of PGS-2 promoter constructs in transient transfection assays of granulosa cells. However, when the E-box Mt was transfected into primary granulosa cell cultures, relative basal CAT activity in the absence of hormone was 15% of the activity produced by the Ϫ195 PGS-2⅐CAT construct. The relative CAT activity induced by LH was 15% of the activity of the nonmutated construct (Ϫ195 PGS-2⅐CAT). Since both basal and induced CAT activity decreased, the fold induction by LH was not affected (Fig. 4A). The Double Mt construct (harboring both the CAAT and E-box mutations) and the E-box Mt exhibited similar basal, as well as LH induced, relative CAT activity. The fold induction of the Double Mt by LH decreased from 9-to 5-fold compared to the Ϫ195 PGS-2⅐CAT (Fig. 4A). These data provide further evidence that the E-box element is an essential DNA cis-element for the transactivation of PGS-2 gene promoter constructs in differentiated granulosa cells.
Relative CAT activities induced by GnRH in cells transfected with the Ϫ195 PGS-2⅐CAT construct versus the C/EBP Mt were similar (40 Ϯ 16 versus 50 Ϯ 10; Fig. 4B). However, transfection of the E-box Mt decreased relative CAT activity induced by GnRH to 15% of the activity produced by the nonmutated construct (Ϫ195 PGS-2⅐CAT). The fold induction by GnRH remained unchanged for all three constructs. CAT activity in the presence or absence of GnRH in the Double Mt was similar and not different from that of the E-box Mt (Fig. 4B). These data FIG. 4

. Role of the CAAT box and the E-box DNA binding elements in the trans-activation of the rat PGS-2 gene.
A, mutations within the CAAT box and the E-box sites (as indicated by lower case letters) were generated with the polymerase chain reaction and oligonucleotides containing mutations within the CAAT box or E-box (see Fig. 1). Primary cultures of differentiated granulosa cells were transiently transfected with the mutated constructs: C/EBP Mt, E-box Mt, and Double Mt. The cells were then treated without (gray bars) or with LH (500 ng/ml; black bars). After 6 h, cells were harvested, lysed, and the cytosolic fractions were assayed for CAT activity. Data are expressed as relative activity (mean Ϯ S.E. of three independent experiments). Fold induction by LH for the pCAT⅐Basic, Ϫ195, C/EBP Mt, E-box Mt, and the Double Mt PGS-2⅐CAT vectors was 1.75-, 9-, 9.8-, 8.8-, and 5.1-fold, respectively. B, granulosa cells transfected with the three mutant constructs were also treated without (gray bars) or with (hatched bars) GnRH (1 M) for 6 h before proteins were collected and CAT activity measured. The data are expressed as relative activity (mean Ϯ S.E. of three independent experiments). Fold induction by GnRH for the pCAT⅐Basic, Ϫ195, C/EBP Mt, E-box Mt, and the Double Mt PGS-2⅐CAT constructs was 1-, 3.3-, 4-, 3.1-, and 2-fold, respectively. further demonstrate that the E-box is required for basal activity, as well as hormone induction, of PGS-2 transgenes in granulosa cell cultures.
CREB Does Not Bind the Ϫ67/Ϫ41 Region of the Rat PGS-2 Promoter-The mouse PGS-2 promoter Ϫ65/Ϫ39 bp oligonucleotide binds both CREB (36), c-Jun (37), and E-box proteins (36) in electrophoretic mobility shift assays. This same region is essential for v-src induction of PGS-2 chimeric genes in NIH 3T3 cells (37). To determine whether the protein-DNA complexes shifted by the rat Ϫ67/Ϫ41 oligonucleotide contained CREB, or other CRE-binding proteins, nuclear extracts of granulosa cells were incubated with an end-labeled rat Ϫ67/Ϫ41 oligonucleotide (Fig. 5A) in the presence or absence of unlabeled consensus CRE oligonucleotide or CREB antibody. The two complexes binding the rat E-box element were not competed by 100-fold excess of CRE; nor were they supershifted with the CREB antibody (Fig. 5B). To directly compare the proteins binding to the mouse and rat CRE/E-box promoter regions, an oligonucleotide corresponding to the mouse CRE/Ebox (Ϫ65/Ϫ39; Fig. 5A) was labeled and incubated with granulosa cell nuclear extracts. Protein-DNA complexes in addition to complex I and II were observed and are denoted as complexes III and IV (Fig. 5C). When an unlabeled CRE consensus oligonucleotide was incubated with the nuclear extracts and the mouse oligonucleotide, complexes III and IV, but not I and II, were decreased (Fig. 5, B and C). The ability of the CREB antibody to shift CREB protein present in the mouse complex IV (Fig. 5C (*)) confirmed the ability of CREB to bind mouse E-box oligonucleotide. CREB was not present in rat or mouse complexes I and II (Fig. 5, B and C). By these in vitro DNA binding assays, it is clear that more proteins bind to this region of the mouse PGS-2 promoter than to the rat promoter, and that one of the proteins is CREB.
USF Binds to the E-box Region of the PGS-2 Promoter-Several class B E-box binding proteins have been identified and shown to bind the CACGTG consensus site (50). These include USF which binds to this region when a thymidine (T) residue precedes the consensus region (TCACGTG) (50). To determine if USF was present in the E-box DNA-protein complexes, USF antibody was added to the binding reactions at increasing concentrations (dilutions of 1:2000, 1:200, and 1:20). Each dilution of the antiserum generated a supershifted complex, which was not observed in the presence of preimmune serum (Fig. 6A). The increasing amount of antibody in the binding reaction reduced the amount of protein/DNA present in both complex I and II suggesting that both protein-DNA complexes contain the transcription factor USF.  4 -6). Thus, USF protein is present in both protein complexes (I and II) that bind the Ϫ67/Ϫ41 oligonucleotide. B, differentiated granulosa cell extracts were exposed to an ovulatory dose of hCG (500 ng/ml). After 0, 2, 7, 12, or 24 h, the cells were lysed and whole cell extracts were prepared. The whole cell lysates (50 g) were separated by SDS-polyacrylamide gel electrophoresis and transferred to nitrocellulose. The membranes were incubated with USF antibody at 1:5000 dilution in 2% milk, 1 ϫ PBS. The blots were then incubated with anti-rabbit IgG conjugated to horse radish peroxidase (1:10,000) and visualized with ECL. The USF protein (43/44 kDa; arrow) is expressed in differentiated granulosa cells throughout a 24-h incubation with hCG.
This study has demonstrated that USF protein binds to the E-box in the rat PGS-2 promoter (Fig. 6A) and that this binding does not vary during follicular development (Fig. 3B). To determine if the USF protein is induced by LH in association with induction of the PGS-2 gene, differentiated granulosa cells were induced with hCG (500 ng/ml) for 2, 7, 12, and 24 h. Whole cell protein extracts collected prior to incubation with hCG (500 ng/ml) and after each time point were analyzed by immunoblot analysis. The amount of USF protein present in whole cell extracts of granulosa cells did not change after incubation with hCG (Fig. 6B). The doublet present on the immunoblot suggests that both the 43-and 44-kDa isoforms of USF are present in the granulosa cell lysates.
Promoters of Several Ovarian Genes Contain an E-box DNA cis-Element-Other genes which are involved in ovarian function contain a similar E-box consensus site in their 5Ј-flanking regions ( Table I). The promoter of the RII␤ gene (regulatory subunit of protein kinase A) contains an E-box that binds both USF and c-myc present in Chinese hamster ovary (CHO) fibroblasts and mouse Nb2a neuroblastoma cell extracts (51). The gene encoding Ad4bp (adrenal 4 binding protein), also known as SF-1, requires an E-box for transcriptional activation of fusion constructs transfected into Y-1 adrenocortical cells and MA-10 testicular Leydig cells (52). Finally, the promoter of the FSH-receptor gene also has a consensus E-box (53) that is important for regulation of the FSH-receptor promoter fusion constructs in rat Sertoli cells (54).
To determine if the E-box elements present in the promoters bound similar proteins as the rat PGS-2 E-box, oligonucleotides corresponding to E-boxes of the human and rat PGS-2, as well as rat SF-1, rat FSH-R, and mouse RII␤ genes were synthesized, end-labeled and incubated with granulosa cell nuclear extracts. The labeled rat PGS-2 E-box oligonucleotide (Ϫ67/ Ϫ41) formed two protein-DNA complexes (I and II) in the presence of nuclear extract as previously discussed (Fig. 7,  panel A, lane 1). Complexes I and II were decreased in the presence of unlabeled competitor DNA corresponding to the rat PGS-2, RII␤, and FSH-receptor E-box regions, but not to a CRE oligonucleotide (Fig. 7, panel A, lanes 2-5). In addition, both complexes were shifted in the presence of USF antibody (1: 2000; Fig. 7, panel A, lane 6).
The labeled human PGS-2 oligonucleotide formed several protein-DNA complexes when incubated with rat granulosa cell nuclear extract (Fig. 7, panel B, lane 7). These complexes were specifically competed by competitor human PGS-2 E-box DNA, but not with either rPGS-2 or RII␤ E-box competitor DNA (Fig.  7, panel B, lanes 8 -10). Consensus CRE competitor DNA prevented formation of all, but two protein-DNA complexes (Fig. 7,  panel B, lane 11). None of the complexes was shifted in the presence of the USF antibody (Fig. 7, panel B, lane 12).
Labeled SF-1 E-box oligonucleotide formed two protein-DNA complexes which were decreased in the presence of SF-1 and rPGS-2 E-box competitor DNA (Fig. 7, panel C, lanes 1-4). These protein-DNA complexes were shifted in the presence of the USF antibody (Fig. 7, panel C, lane 5).
Labeled mouse RII␤ oligonucleotide formed two complexes  5Ј-agg G GTG GGT CAC GTG ACT TTG C 53 C CAC CCA GTG CAC TGA AAC G gga similar to complexes I and II of the rPGS-2 E-box oligonucleotide (Fig. 7, panel D, lane 1). These complexes were decreased in the presence of RII␤, FSH-R, and rPGS-2 competitor DNA, but not in the presence of a CRE oligonucleotide (Fig. 7, panel  D, lanes 2-5). The complexes were shifted in the presence of a USF antibody (Fig. 7, panel D, lane 6). An additional protein-DNA complex is also present, but is not specifically competed by the unlabeled competitor DNA (Fig. 7, panel D, lanes 2-4).
Labeled FSH-receptor DNA formed two complexes similar to complexes I and II formed by the rat PGS-2, as well as the SF-1, E-box oligonucleotides (Fig. 7, panel E, lane 7). Formation of these complexes was prevented by competitor FSH-R, RII␤, and rPGS-2 E-box DNA, but not the consensus CRE oligonucleotide (Fig. 7, panel E, lanes 8 -11). In addition, both complex I and II were shifted in the presence of the USF antibody (Fig.  7, panel E, lane 12).
In summary, promoters for rat PGS-2, mouse PGS-2, RII␤, FSH-R, and SF-1, but not human PGS-2 promoters, contain an E-box region capable of binding the USF protein present in granulosa cell nuclear extracts. The mouse PGS-2 and human PGS-2 promoters also contain a consensus CRE adjacent to the E-box, which binds the CREB protein (33,36). DISCUSSION The specific molecular events controlling the expression of the PGS-2 gene in the ovary are dependent not only on the activation of specific signaling pathways, but also on the stage of follicular development and granulosa cell differentiation (55). Induction of PGS-2 mRNA and protein by protein kinase A (LH) and protein kinase C (GnRH) pathways occurs only in granulosa cells that have differentiated to a preovulatory phenotype (14,24,25,26). This study documents that transcriptional regulation by LH and GnRH of rat PGS-2 promoter transgenes in rat ovarian granulosa cells is dependent on the integrity of a cis-acting E-box element located in the proximal promoter. Disruption of this E-box by a 5Ј deletion to Ϫ52 bp, a point mutation that deletes the C nucleotide at position Ϫ52, or mutation of the consensus E-box sequence from CACGTG to CACtTt within the context of the Ϫ192 bp of the promoter caused marked decreases in the transcriptional activity of chimeric genes transfected into cultured rat granulosa cells. Mutations of a functional C/EBP consensus binding site (Ϫ140/ Ϫ132) (38) within the context of the promoter did not abolish basal or hormonal regulation of the transgenes, indicating that the E-box region interacts with other cis-acting DNA elements located proximal to the CAAT box. Electrophoretic mobility shift assays documented that the transcription factor USF is present in granulosa cell nuclear extracts and binds to an E-box region of the rat PGS-2 promoter. No additional protein-DNA complexes were observed within this region indicating that USF is the primary transcription factor in granulosa cells that binds the E-box region of the rat PGS-2 promoter.
By comparing the binding activities of the rat, mouse, and human PGS-2 E-box regions using rat granulosa cell nuclear extracts, we have shown that the rat E-box region forms two complexes both of which contain USF. The E-box region of the mouse PGS-2 gene also binds USF, but in addition binds CREB and c-Jun (36,37). Despite the ability of the mouse PGS-2 promoter to bind each of these factors, c-Jun is the transcription factor in NIH-3T3 cells that is able to mediate v-src transactivation of mouse PGS-2 transgenes in the absence of a functional E-box (37). A deletion mutant of the human PGS-2 promoter (Ϫ52/59) that disrupts the putative human E-box region also abolishes functional activity of chimeric transgenes genes. However, it is not yet known if the human PGS-2 E-box has functional importance for activity in any of the cell types tested or in conjunction with other regions (NF-B, C/EBP, CRE) reported to confer functional activity (33,34). In addition, it is not yet known which (if any) E-box proteins bind the putative E-box region of the human PGS-2 promoter. Although the human E-box (CACATG) differs from the rat and mouse (CACGTG) and does not bind USF, this sequence has been shown to bind other E-box proteins (50).
Although differences in PGS-2 promoter sequences between species may determine tissue-specific expression of the gene, the observed differences in the PGS-2 E-box regions of rat, mouse, and human do not appear to have major effects on the induction of PGS-2 in granulosa cells of preovulatory follicles. PGS-2 is induced by LH in rat (14,24,25), mouse (data not shown), bovine (56), and presumably human preovulatory granulosa cells. Furthermore, a mouse PGS-2 luciferase construct containing Ϫ371 bp of promoter sequence (36) is activated by LH when transfected into cultured rat granulosa cells (data not shown). Thus, the PGS-2 E-box of each species is likely to be functionally important in granulosa cells with the CRE/ATF binding sites providing additional regulation of activity in the mouse and human genes. Because the PGS-2 gene is usually repressed except in specific cell types and some transformed cell lines, there must also be factors that silence the promoter either by effecting the binding of USF or other regulatory factors.
Although USF was the only protein in granulosa cell extracts that bound to the rat PGS-2 E-box region in detectable amounts, it is unlikely to be the sole factor controlling PGS-2 expression in granulosa cells in response to agonist induction. USF protein levels and DNA binding activities were similar in small antral, preovulatory, and ovulatory granulosa cells exposed to hCG. This is not surprising since USF is ubiquitously expressed in all tissues (57). Therefore, changes in USF activity might be regulated by phosphorylation or by the interaction of USF with other components of the transcriptional machinery that are regulated or modified by the actions of LH and GnRH. Because no consensus phosphorylation sites have been found in the domains of the USF protein required for activation of transcription and because the putative USF protein activation domains are themselves not acidic activation domains, the mechanism of trans-activation by USF remains unclear (58). It appears likely that additional cis-acting factors and unknown co-activators may be involved in the hormonal regulation of the PGS-2 promoter in the rat ovary. Co-activators of USF have been identified in other tissues (59) and in the regulation of viral genes (60), however, the putative co-activator(s) of USF in ovarian cells remain to be determined.
The regulatory role of E-box regions is intriguing because several other genes expressed in ovarian granulosa cells have been shown to contain functional E-box sequences. These include genes that are developmentally regulated at an early stage of ovarian cell differentiation, such as SF-1 (52, 60 -62) and the FSH receptor (63,64), as well as genes that are hormonally regulated at later stages of granulosa cell differentiation, such as RII␤ (43,65) and PGS-2 (15,27,38). As shown herein, the E-box of each of these genes binds USF present in rat granulosa extracts. The different patterns of expression of these genes during follicular development and in other selected tissues indicate that E-box regions exhibit different functional activities by interacting with different upstream regulatory regions and trans-acting factors in a cell specific manner. For example, although the E-box region of the mouse PGS-2 gene binds more proteins in fibroblasts than in granulosa cells, the E-box region of the mouse PGS-2 gene appeared less important for the expression of PGS-2 transgenes in NIH 3T3 cells stimulated by v-src (36), than the E-box region of the rat PGS-2 genes expressed in rat granulosa cells stimulated by either LH or GnRH. Likewise, a region containing the E-box of the RII␤ gene is important for cAMP regulation in rat granulosa cells (43) and binds USF. Although the mouse RII␤ E-box binds more proteins in CHO extracts than in granulosa cell extracts, the magnitude of the effect of the E-box mutations in the RII␤ transgenes expressed in CHO and Nb2a cells (51) was far less than that observed for E-box mutants of SF-1 transgenes expressed in steroidogenic tissues (52) and E-box mutants of PGS-2 transgenes expressed in granulosa cells. Last, despite the presence of E-box proteins in CV-1 cells, SF-1 mRNA is not expresssed in these cells; nor did these cells express SF-1 transgenes (52). Taken together, these observations indicate that the functional potency of the E-box regions of these genes is greater in steroidogenic cells than in fibroblasts and that the differences in potency likely depend on the relative amount and type of E-box binding proteins, as well as specific regions of the promoters and the cell specific factors binding to these regions. Thus, if USF is ubiquitous and constitutively expressed in granulosa cells, other factors must contribute to the developmental and hormone-induced expression of the PGS-2, RII␤, SF-1, and FSH receptor genes at specific stages of granulosa cell differentiation by agonists.
The complexity of the regulation of the PGS-2 gene in multiple tissues is further underscored by the presence of other regions that confer activity in a cell specific manner. Previous studies in our laboratory (38) as well as those of others (33)(34)(35) have indicated that the consensus CAAT site binds C/EBPs and in certain conditions can confer agonist trans-activation of the PGS-2 gene. However, in the present study, when the CAAT site was mutated within the context of the intact promoter, no decrease in basal or agonist stimulated CAT activity was observed. Thus, although the CAAT box is capable of conferring agonist induction to the PGS-2 gene in the absence of the E-box cis-element, this may be due to the ability of C/EBP␣ and C/EBP␤ to interact with the other components of the transcriptional machinery, whereas in the presence of USF, this interaction either does not occur, is not required or based on results with the human PGS-2 promoter, is altered to exert an inhibitory effect. Specifically, the activity of the human PGS-2 promoter in bovine arterial endothelial cells and of the mouse promoter in the osteoblastic cell line, MC3T3-E1, are highly responsive to the C/EBP site in an isoform specific manner (34,35). C/EBP␦, and not C/EBP␣ and C/EBP␤, is induced in bovine arterial endothelial cells by 12-O-tetradecanoylphorbol-3-acetate and lipopolysaccharide. Moreover, when a C/EBP␦ expression vector was cotransfected with a human PGS-2 reporter construct into bovine arterial endothelial cells containing 327 bp of 5Ј-flanking sequence (including C/EBP, CRE, and putative E-box sequences), luciferase activity was markedly increased. Co-transfection with C/EBP␤ and C/EBP␣ vectors decreased the response (34). In granulosa cells, C/EBP␤, rather than C/EBP␦, mRNA, and protein are induced in response to LH, whereas C/EBP␣ is decreased (38). Therefore, C/EBP␤ or C/EBP␣ may be candidates for factors that mediate the rapid decrease in trans-activation of the rat PGS-2 gene in response to the ovulatory LH surge.
In summary, transcriptional regulation of the PGS-2 gene is multifaceted. The trans-acting factors and cis-acting DNA elements that mediate trans-activation of the endogenous PGS-2 gene and chimeric PGS-2 transgenes appear to be dependent on the cell type, the agonists used to stimulate cellular signaling pathways and the species specific promoter elements. In this study we have shown that the integrity of the E-box region of the rat PGS-2 gene is required for expression in granulosa cells and appears to interact with upstream cis-acting elements other than the CAAT box to confer hormonal regulation of this gene in rat granulosa cells. The E-box region of the rat PGS-2 promoter binds USF (as do E-box regions of other genes regulated in the ovary), but does not contain ATF/CRE activity found in the human and mouse PGS-2 promoters.