Cloning and Characterization of a Specific Interleukin (IL)-13 Binding Protein Structurally Related to the IL-5 Receptor α Chain*
- Daniel Caput,
- Patrick Laurent,
- Mourad Kaghad,
- Jean-Michel Lelias,
- Sylvie Lefort,
- Natalio Vita and
- Pascual Ferrara‡
- ‡ To whom correspondence should be addressed. Tel: (33) 61 00 40 00; Fax: (33) 61 00 40 01; E-mail: pascual.ferrara{at}tls1.elfsanofi.fr.
Abstract
Interleukin-13 (IL-13) is a cytokine secreted by activated T lymphocytes that shares many, but not all, biological activities with IL-4. These overlapping activities are probably due to the existence of common receptor components. Two proteins have been described as constituents of the IL-4 receptor, a ∼140-kDa glycoprotein (IL-4R) and the γ chain (γc) of the IL-2 receptor, but neither of these proteins binds IL-13. We have cloned a cDNA encoding an IL-13 binding protein (IL-13R) from the Caki-1 human renal carcinoma cell line. The cloned cDNA encodes a 380-amino acid protein with two consensus patterns characteristic of the hematopoietic cytokine receptor family and a short cytoplasmic tail. The IL-13R shows homology with the IL-5 receptor, and to a lesser extent, with the prolactin receptor. COS-7 cells transfected with the IL-13R cDNA bind IL-13 with high affinity but do not bind IL-4. COS-7 cells co-transfected with the cloned IL-13R cDNA and IL-4R cDNA resulted in the reconstitution of a small number of receptors that recognized both IL-4 and IL-13. Reverse transcription-polymerase chain reaction analysis detected the receptor transcript only in cell lines known to bind IL-13.
Footnotes
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↵* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBank™/EMBL Data Bank with accession number(s) X95302[GenBank].
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↵1 The abbreviations used are:
- IL
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interleukin
- PCR
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polymerase chain reaction
- γc
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γ chain
- R
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receptor.
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- Received February 5, 1996.
- Revision received April 5, 1996.
- © 1996 by The American Society for Biochemistry and Molecular Biology, Inc.
