Identification of the Nuclear Localization Signal of the POU Domain Protein Tst-1/Oct6

doi:10.1074/jbc.271.29.17512

Identification of the Nuclear Localization Signal of the POU Domain Protein Tst-1/Oct6*

From the ^‡ Zentrum für Molekulare Neurobiologie, Universität Hamburg, Martinistrasse 52, D-20246 Hamburg, Federal Republic of Germany and the
^§ Howard Hughes Medical Institute, School of Medicine, University of California at San Diego, La Jolla, California 92093-0648

^∥ To whom correspondence should be addressed:
ZMNH, Interim I, Pav. 22, Martinistr. 52, D-20246 Hamburg, Germany.
Tel.: 49 40 4717 4708; Fax: 49 40 4717 4774.

Abstract

POU domain proteins are important regulators of development and terminal differentiation based upon their transcriptional activity in the nucleus. Here, we analyzed the mechanism underlying the nuclear localization of Tst-1/Oct6, a member of this family that regulates events during neurogenesis and myelination. Nuclear localization of Tst-1/Oct6 was dependent on the POU domain, as its deletion prevented access to the nucleus, whereas its transfer to the amino terminus of β-galactosidase was sufficient to prompt nuclear accumulation of this normally cytosolic protein. Interestingly, nuclear localization and high affinity DNA binding were two independent functions of the POU domain and could be separated in several mutants. While specific high affinity binding to DNA required the presence of both the POU-specific and the POU homeodomain, the POU-specific domain was dispensable for nuclear localization of Tst-1/Oct6. Rather, the nuclear localization function was selectively contained within the POU homeodomain. Specifically, a basic cluster (GRKRKKRT) preceding helix 1 of the homeodomain was shown by deletion mutagenesis to be involved in the nuclear localization of Tst-1/Oct6. This sequence, which is highly conserved among POU domain proteins, was by itself capable of translocating β-galactosidase to the nucleus defining it as the bona fide nuclear localization signal of Tst-1/Oct6 and presumably other POU domain factors.

Footnotes

↵^¶ Investigator with Howard Hughes Medical Institute.
↵* This work was supported by Grant We 1326/5-1 from the Deutsche Forschungsgemeinschaft (to M. W.) and a grant from the National Institutes of Health (to M. G. R.). The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
↵¹ The abbreviations used are:

NLS

nuclear localization signal

X-Gal

5-bromo-4-chloro-3-indolyl β-D-galactoside

PBS

phosphate-buffered saline.
- Received March 7, 1996.