Serine Phosphorylation, Chromosomal Localization, and Transforming Growth Factor-β Signal Transduction by Human bsp-1*
- Robert J. Lechleider‡§,
- Mark P. de Caestecker‡,
- Anindya Dehejia¶,
- Mihael H. Polymeropoulos¶ and
- Anita B. Roberts‡
- From the ‡ Laboratory of Chemoprevention, NCI, National Institutes of Health, Bethesda, Maryland 20892-5055 and
- ¶ Gene Mapping Unit, Laboratory of Genetic Disease Research, National Center for Human Genome Research, Bethesda, Maryland 20892
- § Supported in part by a Damon Runyon-Walter Winchell Cancer Research Fund physician scientist award. To whom correspondence should be addressed: Laboratory of Chemoprevention, NCI, National Institutes of Health, 41 Library Dr., Bethesda, MD 20892-5055. Tel.: 301-496-5391; Fax: 301-496-8395; E-mail: rjl{at}helix.nih.gov
Abstract
The transforming growth factor-β (TGF-β) superfamily regulates a multitude of cellular and developmental events. TGF-β family ligands signal through transmembrane serine/threonine kinase receptors whose downstream effectors are largely unknown. Using genetic data from the fruit fly, we have identified a downstream effector of TGF-β-induced signaling. TGF-β signaling protein-1 (BSP-1) is rapidly phosphorylated in response to TGF-β. Localization of bsp-1 to chromosome 4q28 suggests a role in carcinogenesis. These data suggest that BSP-1 is the prototype of a new class of signaling molecules.
Footnotes
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↵* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBank™/EMBL Data Bank with accession number(s) U57456[GenBank].
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↵1 The abbreviations used are:
- TGF-β
-
transforming growth factor-β
- BSP-1
-
TGF-β signaling protein-1
- EST
-
expressed sequence tag
- 5′-UTR
-
5′-untranslated region
- PCR
-
polymerase chain reaction
- GST
-
glutathione S-transferase
- bp
-
base pair
- kb
-
kilobase(s)
- BMP-2
-
bone morphogenetic protein-2.
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↵2 D. Slonim, L. Stein, L. Kruglyak, and E. Lander, unpublished software.
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↵3 R. J. Lechleider, data not shown.
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↵4 M. P. de Caestecker, P. Hemmati, and R. J. Lechleider, unpublished results.
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- Received April 22, 1996.
- Revision received May 23, 1996.
- © 1996 by The American Society for Biochemistry and Molecular Biology, Inc.
