The response of renal tubular epithelial cells to physiologically and chemically induced growth arrest.

Cells respond to a variety of stresses by activating the transcription of a battery of “acute phase” or “stress response” genes. The nature of this response is tailored to the nature of the stress. The extent to which physiologically and pathophysiologically induced growth arrest share common genomic responses is unclear. We therefore compared the effects of a physiologically induced (serum and nutrient depletion) and a chemically induced (2-Br-bis-(GSyl)HQ and 2-Br-6-(GSyl)HQ) stress in renal tubular epithelial cells (LLC-PK1). The response to physiological stress, induced by serum depletion, involves growth arrest characterized by an inhibition of DNA synthesis that occurs in the absence of a decrease in histone mRNA or an increase in gadd153 mRNA, one of the growth arrest and DNA damage inducible genes. In contrast, the chemical-induced stress involves growth arrest accompanied by a decrease in histone mRNA, particularly core histone H2B and H2A mRNA, and the induction of gadd153 Chemical-induced changes in histone mRNA inversely correlate to changes in the expression of a stress gene, hsp70, whose expression is dependent upon the maintenance of appropriate nucleosomal structure.

transcription of a battery of "acute phase" or "stress response" genes. The nature of this response is tailored to the nature of the stress. The extent to which physiologically and pathophysiologically induced growth arrest share common genomic responses is unclear. We therefore compared the effects of a physiologically induced (serum and nutrient depletion) and a chemically induced (2-Br-bis-(GSyl)HQ and 2-Br-6-(GSyl)HQ) stress in renal tubular epithelial cells (LLC-PK 1 ). The response to physiological stress, induced by serum depletion, involves growth arrest characterized by an inhibition of DNA synthesis that occurs in the absence of a decrease in histone mRNA or an increase in gadd153 mRNA, one of the growth arrest and DNA damage inducible genes. In contrast, the chemical-induced stress involves growth arrest accompanied by a decrease in histone mRNA, particularly core histone H2B and H2A mRNA, and the induction of gadd153. Chemical-induced changes in histone mRNA inversely correlate to changes in the expression of a stress gene, hsp70, whose expression is dependent upon the maintenance of appropriate nucleosomal structure.
Cells respond to a variety of stresses by activating the transcription of a battery of "acute phase" or "stress response" genes (1,2). The nature of this response is tailored to the nature of the stress. For example, serum-and nutrient-deprived cells usually enter a quiescent state (G 0 ) that requires both the up-regulation and down-regulation of genes involved in growth control. In contrast, the initial response to DNA-damaging agents includes arrest of cell cycle progression, presumably to facilitate the transcription of a battery of genes involved in the DNA repair process (3) prior to DNA replication (G 1 arrest) and cell division (G 2 arrest). The extent to which physiologically and pathophysiologically induced growth arrest share common genomic responses is unclear and is the focus of the present studies.
The conjugation of ortho-, or para-quinones with GSH results in the formation of conjugates that frequently exhibit more potent toxicological activity than the parent quinone (4). GSH conjugates of polyphenolics are also formed as metabolites of a variety of "non-genotoxic" carcinogens (5-7). As a model of quinone-thioether-mediated toxicity we have been investigating the cellular and molecular responses to 2-Br-bis-(glutathion-S-yl)hydroquinone (2-Br-bis-(GSyl)HQ) 1 and 2-Br-6-(glutathion-S-yl)hydroquinone (2-Br-6-(GSyl)HQ). 2-Br-bis-(G-Syl)HQ (30 mol/kg) causes margination of heterochromatin and loss of chromatin staining when administered to male Fischer 344 rats (8), and in renal tubular epithelial cells (LLC-PK 1 ) it causes the formation of single strand breaks in DNA (9), rapid inhibition of DNA synthesis (10), and the induction of the growth arrest and DNA damage-inducible gene, gadd153 (10). Quinone-thioethers therefore provide a useful model with which to investigate chemically induced growth arrest and the consequences of chemically induced chromatin disruption. We have therefore compared physiologically induced (serum and nutrient depletion) and chemically induced (2-Br-bis-(GSyl)HQ and 2-Br-6-(GSyl)HQ) growth arrest in renal tubular epithelial cells (LLC-PK 1 ) and determined some of the factors involved in regulating gene expression in response to the chemically induced stress. We report that the response to physiological stress (serum deprivation) involves growth arrest characterized by an inhibition of DNA synthesis in the absence of changes in histone or gadd153 mRNA. In contrast, the chemically induced stress involves growth arrest accompanied by a decrease in histone mRNA, particularly core histone H2A and H2B mRNA, and the induction of gadd153.

MATERIALS AND METHODS
Chemicals and Probes-Unless specified otherwise, chemicals were obtained from Sigma. 2-Br-6-(GSyl)HQ and 2-Br-bis-(GSyl)HQ were synthesized according to established methodology (11). Ethylene glycolbis-(␤-aminoethyl ether) N,N,NЈ,NЈ-tetraacetic acid (EGTA-AM) was purchased from Molecular Probes, Inc. (Eugene, OR). A plasmid pBluescript containing 600-bp fragment of cDNA of gadd153 was kindly provided by Dr. Nikki J. Holbrook (NIA, National Institutes of Health, Gerontology Research Center). The cDNA probe for histone H2B was purchased from Oncor (Gaithersburg, MD), and the probes for histone H2A and H3 were available from the American Type Culture Collection (Rockville, MD). Probes for histone H1 and H4 were kindly provided by Drs. Gary and Janet Stein (Dept. Cell Biology, University of Massachusetts), who constructed plasmids containing fragments of the histone genes. pFNC16A (H1) and pF0002 (H4) were transformed into a competent cell line (DH5␣; Life Technologies, Inc.  20 mM HEPES, and 1.8 mM CaCl 2 ⅐2H 2 O) and treated with 2-Br-6-(GSyl)HQ or 2-Br-bis-(GSyl)HQ in EBSS or DMEM. With the exception of EGTA-AM (50 M), which was added to cultures 30 min prior to drug treatment, all inhibitors were incubated with LLC-PK 1 cells for 1 h prior to drug exposure.
Determination of DNA Synthesis-Cells were seeded at a density of 2 ϫ 10 5 cells/well in six-well plates and were used following overnight culture. Cells were either incubated in the presence of catalase (10 units/ml) and 2-Br-bis-(GSyl)HQ (200 M) for 2 h or pretreated with deferoxamine (10 mM) for 1 h, washed in DMEM-Hepes, and then exposed to 2-Br-bis-(GSyl)HQ (200 M) for 2 h. Cultures were then washed twice with DMEM containing Hepes without fetal bovine serum. Each well was pulsed with 5 Ci/ml of [ 3 H]thymidine (specific activity, 58 Ci/mmol). After incubation with [ 3 H]thymidine for 4 h, cells were washed three times with phosphate-buffered saline, pH 7.4, and 5% trichloroacetic acid was added. Cells were harvested by gentle scraping, and centrifuged for 5 min at 14,000 rpm. The supernatant was removed, and the pellet was washed by adding 5% trichloroacetic acid to remove unincorporated [ 3 H]thymidine. The same procedure was repeated three times. A final washing with absolute ethanol was performed to remove residual trichloroacetic acid, and the pellet was dried. Dried pellets were dissolved in 1 ml of 0.1 N NaOH and used for the determination of protein concentration and radioactivity by liquid scintillation spectroscopy. Protein concentrations were determined by the Bio-Rad protein method using bovine serum as the standard.
Northern Blot Analysis-Total RNA was isolated using RNAzol (Cinna/Biotecx Laboratory International Inc., Friendswood, TX) and the procedure of Chomczynski and Sacchi (12). For Northern blot analysis, RNA was electrophoresed in 1.0% agarose gel containing formaldehyde and transferred to Zeta Probe membranes (Bio-Rad). cDNA probes were labeled with [ 32 P]dCTP (ICN, Costa Mesa, CA) by the random primer method (13) using a kit (Boehringer Mannheim, Mannheim, Germany). All blots were hybridized in 0.25 M Na 2 HPO 4 , pH 7.2, and 7% SDS overnight at 65°C. Blots were washed twice with 25 mM Na 2 HPO 4 , pH 7.2, and 5% SDS at 65°C for 30 min and twice with 25 mM Na 2 HPO 4 , pH 7.2, and 1% SDS at 65°C for 30 min. All blots were rehybridized with the cDNA probe for 7 S rRNA to correct for potential variations in RNA loading by stripping the original probe with 0.1 ϫ SSC and 0.5% SDS at 95°C for 0.5-1 h. Autoradiography was performed using Kodak XAR-5 film (Sigma) exposed for between 1 h to 5 days, depending on the probe, at Ϫ70°C with intensifying screens.
Quantitation of Autoradiograms-The data from Northern blot analysis were quantified by densitometer (Imaging Research Inc., Ontario, Canada) or with a Packard InstantImager electronic autoradiography system (Packard Instrument Co., Meridien, CT). All densitometric and electronic values were normalized to 7 S rRNA obtained from the same analysis.

Nutrient Depletion-mediated Growth Arrest: Effects on the
Growth Arrest and DNA Damage-inducible Gene, gadd153, and Histone mRNA in Renal Tubular Epithelial Cells-Depriving cells of serum and nutrients was sufficient to induce gadd153 expression (Fig. 1). Supplementation of EBSS with glucose failed to overcome gadd153 induction, but DMEM, with or without added serum, prevented the increase in gadd153 (Fig. 1). Thus, the induction of gadd153 in physiologically stressed LLC-PK 1 cells appears to be a response to the absence of specific nutrients rather than to an absence of glucose or serum factors. Although incubating cells in DMEM in the absence of serum did not induce gadd153 expression, there was a significant (ϳ98%) reduction in [ 3 H]thymidine incorporation into newly synthesized DNA (Fig. 2) indicative of a state of growth arrest. Because histone synthesis is tightly coupled to DNA synthesis, we examined the effects of this physiologically induced state of growth arrest on the expression of histone mRNA. Neither core (H2A, H2B, H3, H4) nor linker (H1) mRNA levels were decreased in cells grown in the absence of serum and/or nutrients ( Fig. 3; for illustrative purposes, only data for H2B mRNA are presented). Thus, nutrient depletion causes growth arrest and gadd153 expression, and although DNA synthesis is inhibited, histone mRNA levels are unaffected. To determine whether LLC-PK 1 cells respond to chemical-induced growth arrest in a similar manner to the physiological (nutrient depletion) growth arrest, we examined the cellular response to chemical toxicants known to cause both DNA damage and growth arrest.
Linker Histone (H1) mRNA Down-regulation in Response to Quinone-thioether-mediated Cytotoxicity-Quinone-thioethers cause rapid disruption to chromatin in vivo (8)  hydroquinone also cause a rapid inhibition of DNA synthesis and induce gadd153 expression in LLC-PK 1 cells (10). We therefore utilized this model of chemically induced cell stress to compare the cellular response to physiologically and chemically induced growth arrest by examining the effects of quinonethioethers on the expression of histone mRNA. 2-Br-bis-(GSyl)HQ and 2-Br-6-(GSyl)HQ caused a time-and concentration-dependent decrease in linker (H1) histone mRNA expression (Fig. 4). Suppression of H1 mRNA levels was maximal between 2 and 5 h after exposure of LLC-PK 1 cells to a 200 M concentration of either 2-Br-bis-(GSyl)HQ or 2-Br-6-(GSyl)HQ, and the latter conjugate was more potent than the former at decreasing the levels of H1 mRNA.
The Effects of Combined Nutrient Depletion and Chemical Stress on gadd153 and Histone H2B mRNA-It seemed possible that differences in the cellular response to nutrient deprivation and chemical challenge might simply reflect different points on a continuum of a single stress response. In other words, the chemical stress, being more intense than the physiological stress, elicits a more intense stress response. LLC-PK 1 cells were therefore treated with increasing concentrations of 2-Br-6-(GSyl)HQ for 2 h in either EBSS (nutrient-deprived cells) or DMEM. Interestingly, 2-Br-6-(GSyl)HQ-mediated decreases in histone H2B mRNA were less severe under conditions of nutrient depletion (Fig. 7). For example, at 50 M 2-Br-6-(GSyl)HQ, H2B expression decreased 55% in DMEM but only 31% in EBSS. Moreover, nutrient depletion alone appears sufficient to induce maximal gadd153 expression in LLC-PK 1 cells. Thus, after culturing cells for 2 h in EBSS, gadd153 mRNA levels are 6-fold higher than in cells cultured in DMEM (Fig. 8). The addition of 2-Br-6-(GSyl)HQ to EBSS actually decreases the gadd153 response to 86% (50 M) and 79% (100 M) of EBSS alone. In contrast, the chemical stress (2-Br-6-[GSyl]HQ) induces gadd153 expression only 3-fold in DMEM (Fig. 8). Finally, the ability of 2-Br-6-(GSyl)HQ to attenuate the gadd153 response is not merely a consequence of delaying the stress response. We examined gadd153 expression out to 10 h. Nutrient depletion (EBSS) caused a continual increase in gadd153 mRNA throughout the experiment (27-fold increase), and 2-Br-6-(GSyl)HQ blunted the response to nutrient depletion (11-fold increase), which reached a plateau by 10 h (Fig. 9). Clearly, the effects of nutrient depletion and chemical stress in LLC-PK 1 cells are not additive, indicating that the growth arrest response to chemical stress (DNA damage) and nutrient deprivation are different.
Correlation between H2B Histone and hsp70 Expression-Because changes in chromatin structure are coupled to changes in chromatin function (gene expression) we examined the effects of 2-Br-6-(GSyl)HQ on hsp70 expression, a stress gene whose expression is dependent upon the maintenance of appropriate nucleosomal structure (14,15). H2B histone mRNA levels in LLC-PK 1 cells exposed to increasing concentrations of 2-Br-6-(GSyl)HQ were inversely correlated with hsp70 mRNA (Fig. 10)

. Effects of nutrient deprivation and 2-Br-6-(glutathion-
S-yl)hydroquinone on core histone H2B mRNA expression in renal tubular epithelial cells. LLC-PK 1 cells were exposed for 2 h to 50, 100, 200, or 400 M concentrations of 2-Br-6-(glutathion-S-yl)hydroquinone in either EBSS (q) or DMEM (E). Total RNA was extracted, separated, and examined by Northern blot analysis. The level of 7 S rRNA was used as the internal control. Experiments were repeated a minimum of three times and produced similar results.

FIG. 9. Effects of nutrient deprivation and the combination of nutrient deprivation and 2-Br-6-(glutathion-S-yl)hydroquinone on gadd153 mRNA expression in renal tubular epithelial cells.
LLC-PK 1 cells were exposed for 2, 4, 6, 8, or 10 h to either EBSS alone (q) or EBSS and 200 M 2-Br-6-(glutathion-S-yl)hydroquinone (E). Total RNA was extracted, separated, and examined by Northern blot analysis. The level of 7 S rRNA was used as the internal control. Experiments were repeated a minimum of three times and produced similar results. g/ml) and then exposed to either 2-Br-bis-(GSyl)HQ or 2-Br-6-(GSyl)HQ. Under these conditions, core (Fig. 11) and linker histone mRNA levels (data not shown) were almost completely suppressed. Because the results obtained from both conjugates were essentially identical, only data for 2-Br-6-(GSyl)HQ are presented. To examine whether the decrease in histone mRNA requires protein synthesis (transcription factors) LLC-PK 1 cells were pretreated for 1 h with cycloheximide (50 g/ml) and then incubated with 2-Br-bis-(GSyl)HQ or 2-Br-6-(GSyl)-HQ. Inhibiting protein synthesis with cycloheximide partially blocked the decrease in histone mRNA (Fig. 11), indicating that the regulation of histone mRNA levels following chemical stress in LLC-PK 1 cells is mediated in part at the post-transcriptional level.
The Role of Iron, Hydrogen Peroxide, and Calcium in Quinone-thioether-mediated Decreases in Histone Gene Expression-Growth arrest induced by quinone-thioethers, and not by nutrient deprivation, causes a corresponding decrease in histone gene expression. The next series of experiments was therefore carried out to ascertain factors involved in down-regulating histone gene expression. The cytotoxicity of 2-Br-bis-(GSyl)HQ and 2-Br-6-(GSyl)HQ in LLC-PK 1 cells involves the generation of reactive oxygen species (9) most likely via the iron-catalyzed Haber-Weiss reaction, in which O 2 . undergoes dismutation to form hydrogen peroxide, and reduces Fe 3ϩ to Fe 2ϩ . The hydrogen peroxide then reacts with Fe 2ϩ to generate the highly reactive hydroxyl radical, which may be the species responsible for the DNA damage. To determine whether irondependant events are related to the decrease in histone mRNA, we investigated the effects of deferoxamine and catalase on histone gene expression. The chelation of iron with deferoxamine decreased H2B mRNA in the absence of added conjugate (Fig. 12), and no further decreases in H2B mRNA were observed when 2-Br-bis-(GSyl)HQ (data not shown) and 2-Br-6-(GSyl)HQ were added after pretreatment of cells with deferoxamine (Fig. 12). Similar effects were seen with H1, H2A, H3, and H4 mRNA (data not shown). Concomitant with the decrease in H2B mRNA, DNA synthesis was significantly inhibited in deferoxamine-pretreated cells (Fig. 13). Scavenging hydrogen peroxide with catalase totally prevented 2-Br-bis-(GSyl)HQ-mediated decreases in H2B mRNA (Fig. 12). Chelation of intracellular calcium with the cell-permeant EGTA-AM decreased levels of H2B mRNA in the presence and absence of 2-Br-bis-(GSyl)HQ (Fig. 14). Similar effects were seen with H1, H2A, H3, and H4 mRNA (data not shown). Therefore, 2-Br-bis-(GSyl)HQ activates a genomic stress response, involving decreases in histone gene expression, via a signaling pathway that may include iron, calcium, and reactive oxygen species. DISCUSSION DNA synthesis is rapidly inhibited in serum-deprived subconfluent cultures of renal proximal tubular epithelial cells (LLC-PK 1 ) (Fig. 1), but this physiological mode of growth arrest is insufficient to induce gadd153 expression (Fig. 2). However, removal of serum and nutrients, including glucose, from the culture medium is sufficient to induce the expression of gadd153 (Fig. 2). Chen et al. (16) also noted that culturing LLC-PK 1 cells in EBSS induces the expression of gadd153. The addition of glucose to serum-and nutrient-deprived LLC-PK 1

FIG. 11. Effect of cycloheximide and actinomycin D on 2-Br-6-(glutathion-S-yl)hydroquinone-mediated decreases in core histone H2B mRNA expression in renal tubular epithelial cells.
LLC-PK 1 cells were incubated in DMEM (control) with cycloheximide (50 g/ml) or actinomycin D (10 g/ml) for 1 h prior to treatment for 2 h with 200 M 2-Br-6-(glutathion-S-yl)hydroquinone. Total RNA was extracted, separated, and examined by Northern blot analysis. The level of 7 S rRNA was used as the internal control. Experiments were repeated a minimum of three times and produced similar results.  10. Comparative effect of 2-Br-6-(glutathion-S-yl)hydroquinone on core histone H2B and hsp70 mRNA expression in renal tubular epithelial cells. LLC-PK 1 cells were exposed for 2 h to 50, 100, 200, or 500 M concentrations of 2-Br-6-(glutathion-S-yl)hydroquinone. Total RNA was extracted, separated, and examined by Northern blot analysis. The level of 7 S rRNA was used as the internal control. Experiments were repeated a minimum of three times and produced similar results. cells did not overcome the induction of gadd153 (Fig. 1). Glucose is not essential for energy production in cultured cells (17), and glutamine, not glucose, is the major energy source in HeLa cells (18). The mitochondrial glutamine to ␣-ketoglutarate pathway is also the major regulatory site of ammoniagenesis in LLC-PK 1 cells (19). Increased expression of gadd153 occurs in H4-II-E rat hepatoma cells grown in a limited amino acid medium (20). Glutamine can also serve as a precursor for pyrimidine nucleotides, via the glutamate-aspartate transamination reaction, and cells grow readily in sugar-free medium supplemented with uridine or cytidine (17). The absence of glutamine, rather than glucose, probably initiates gadd153 expression in LLC-PK 1 cells, and changes in the nucleotide pool may be a signal for gadd153 expression. Gadd153 is induced by growth arrest and DNA damage (3), and the nucleotide pool will also decline as DNA repair is initiated in cells experiencing DNA damage, again signaling gadd153 expression. The p53dependent G 0 -G 1 cell cycle arrest is induced by ribonucleotide depletion (21), suggesting that p53 may serve as a ribonucleotide sensor. However, although p53 lies upstream of gadd45 (22), it does not appear to control gadd153 expression (23).
The expression of histone genes is tightly coupled to DNA replication, but the levels of histone mRNA were unchanged in both serum-, and nutrient-deprived LLC-PK 1 cells (Fig. 3), despite significant reductions in DNA synthesis (Fig. 2). Although changes in the level of histone mRNA occur between G 1 and S phase cells, the signal for rapid histone mRNA degradation appears to be confined to the S-G 2 border (24,25), and histone mRNA levels only decline rapidly as cells exit the S phase of the cell cycle. Moreover, the half-life of histone mRNA is comparatively long during S phase, ranging from 110 min (25) to 4 -5 h (26).
In contrast to the lack of effects of serum and nutrient deprivation on gadd153 and histone mRNA, chemical-induced growth arrest of LLC-PK 1 cells causes a decrease in both core and linker histone mRNAs  and an increase in gadd153 mRNA (Figs. 8 and 9). Differences in the cellular response to nutrient deprivation and chemical challenge might simply reflect different points on a continuum of a single stress response. We therefore compared the effects of a chemical stress (2-Br-6-(GSyl)HQ exposure in DMEM) and the combination of chemical stress under conditions of nutrient depletion (2-Br-6-[GSyl]HQ exposure in EBSS). However, not only are the two stresses not additive, but decreases in H2B expression are attenuated when the chemical and nutrient stress are combined (Fig. 7). In addition, nutrient deprivation for 2 h causes a 6-fold increase in gadd153 mRNA, and the combination of nutrient deprivation and 2-Br-6-(GSyl)HQ actually decreases the gadd153 response (Fig. 8). The ability of 2-Br-6-(GSyl)HQ to attenuate the gadd153 response is not simply a consequence of a delay in the stress response. Nutrient depletion causes a continual increase in gadd153 mRNA, whereas the combination of nutrient depletion and 2-Br-6-(GSyl)HQ blunts the gadd153 response by 60% (Fig. 9). The chemical challenge, therefore, appears to interfere with pathways utilized by the cell to signal the physiological stress to the nucleus. Whether the same, albeit compromised, pathways are utilized to signal the chemical stress response is unclear, but likely involves the recruitment of alternative signaling pathways. Nonetheless, the genomic response to nutrient depletion and chemically induced growth arrest in LLC-PK 1 cells is clearly not additive.
Chromatin structure plays an important role in regulating gene expression (27)(28)(29)(30). For example, histones play an important role in maintaining chromatin structure and are intimately involved in the regulation of gene transcription (31,32). The molecular mechanisms regulating gene expression in eukaryotes, therefore, include specific roles for the histone proteins. For example, when core histone H2A or H2B synthesis is repressed, the promoter elements of several inducible genes, which are normally repressed because of their incorporation into nucleosomes, become accessible to the basal transcriptional machinery (33,34). Histones are therefore essential for nucleosomal structure and function. Interestingly, core histone H2A and H2B mRNAs are more sensitive to quinone-thioetherinduced DNA damage than core histone H3 and H4 mRNA (Figs. 5 and 6). Because the H3/H4 histone tetramer forms the central core of the nucleosome, with H2A/H2B heterodimers capping each end of the core, H2A/H2B dimers are more readily displaced from the nucleosomal core than H3 and H4 (35). Histone mRNAs are rapidly degraded via the activation of a specific mRNA destabilization process (36) that is autoregulated by histone proteins and initiated by increases in cytoplasmic histones (37). Both core and linker histones induce the destabilization of histone H4 mRNA (38). Release of histones from nucleosomes during quinone-thioether-mediated nuclear disruption may therefore contribute to decreases in histone mRNA levels. Alternatively, quinones have been shown to inhibit ribonucleotide reductase (39), the first step in DNA synthesis. Such an effect may lead to a down-regulation in histone gene expression. However, this mechanism would not explain the differential effects of 2-Br-6-(GSyl)HQ on H2A/H2B and H3/H4 mRNA. Moreover, the activity of ribonucleotide reductase is reduced in serum-starved hamster kidney 21/C13 cells (40), and serum deprivation does not decrease histone mRNA in LLC-PK 1 cells (Fig. 3). Chemically induced changes to chromatin structure might therefore provide an important mechanism by which so called "non-genotoxic" carcinogens influence gene expression and cell growth. Indeed, a variety of chemicals, including sodium butyrate, trichostatin A, and staurosporine, are known to alter the post-translational modification of histones, with corresponding changes in nucleosomal structure and function (41)(42)(43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53).
The compaction of nucleosomal templates containing histones H2A and H2B is more extensive than templates containing only H3/H4 tetramers, and removal of H2A and H2B from nucleosomal arrays enhances gene activity (54). Moreover, under conditions of efficient chromatin assembly, the hsp70 promoter is transcriptionally silent in Xenopus oocytes, and a role for chromatin structure in regulating the hsp70 promoter has been proposed (14,15). Consistent with this scenario, levels of histone H2B mRNA in LLC-PK 1 cells exposed to increasing concentrations of 2-Br-6-(GSyl)HQ were inversely correlated with hsp70 mRNA (Fig. 10). Perhaps the increase in cytotoxicity at higher concentrations of 2-Br-6-(GSyl)HQ leads to an inhibition of protein synthesis with a complementary increase in histone mRNA stability by mechanisms described above. Alternatively, disruption to chromatin structure at higher concentrations of 2-Br-6-(GSyl)HQ may be sufficient to suppress the expression of hsp70, which appears to require precise assembly of the promoter template into chromatin for active transcription (14). This would imply that the structural requirements for gene transcription are less stringent for some genes than others.
Inhibiting protein synthesis with cycloheximide attenuated decreases in histone H2B mRNA (Fig. 11), consistent with the finding that the down-regulation of histone H1 caused by 1-␤-D-arabinofuranosylcytosine is also prevented by cycloheximide (55). Inhibition of protein synthesis has been reported to prevent rapid degradation of histone mRNA (37), possibly by decreasing the interaction of a 45-kDa stem-loop-binding protein with the 3Ј-end of histone mRNA, which may be necessary for histone mRNA degradation (56). Thus, the regulation of histone mRNA following chemical stress in LLC-PK 1 cells is in part mediated at the post-transcriptional level. Inhibition of transcription with actinomycin D virtually abolished histone H2B expression (Fig. 11), indicating that active transcription is required to maintain histone mRNA levels in these cells.
Quinone-thioether-mediated DNA damage and cytotoxicity requires the availability of Fe 2ϩ (9). Treatment of LLC-PK 1 cells with deferoxamine to chelate intracellular Fe 2ϩ decreases H2B histone mRNA (Fig. 12) and DNA synthesis (Fig. 13). Iron is essential to maintain ribonucleotide reductase activity (57), the rate-limiting step in DNA synthesis (58), and deferoxamine has been shown to inhibit this enzyme (59). Iron is also required to maintain synthesis of the cell cycle-regulated protein p34 cdc2 (60). Thus, intracellular iron concentrations, either directly or by modulating p34 cdc2 synthesis and ribonucleotide reductase activity, influence cell cycle progression and histone gene expression. H2B histone mRNA levels in deferoxaminetreated cells were unaffected by treatment with 2-Br-6-(GSyl)HQ (Fig. 12), which is consistent with the ability of deferoxamine to prevent 2-Br-6-(GSyl)HQ-mediated DNA damage (10) and cytotoxicity (9,10). Calcium also plays an important role in controlling cell proliferation and regulates progression through several cell cycle checkpoints, including the G 1 /S phase transition, the G 2 /M phase transition, and exit from mitosis (61). Calcium is also required for p34 cdc2 histone H1 kinase activity (62). Therefore, decreasing intracellular calcium concentrations will delay cell cycle progression, decreasing the requirement for histone protein synthesis as DNA synthesis is suppressed. Chelation of intracellular calcium in LLC-PK 1 cells with EGTA-AM decreases the constitutive expression of histone H2B mRNA and potentiates 2-Br-6-(GSyl)HQ-mediated decreases in H2B mRNA (Fig. 14). Although the relationship between intracellular calcium concentrations and histone gene expression is likely to be complex, our findings establish a link between the two.
In summary, renal tubular epithelial cells respond differently to physiologically and chemically induced stress. The response to physiological stress, induced by a combination of serum and nutrient depletion, involves growth arrest characterized by an inhibition of DNA synthesis that occurs in the absence of a decrease in histone mRNA or an increase in gadd153 mRNA. In contrast, a chemical-induced stress mediated by exposure of cells to quinone-thioethers involves growth arrest accompanied by a decrease in histone mRNA, particularly core histone H2B and H2A mRNA, and the induction of gadd153. Chemical-induced expression of hsp70 mRNA appears to be linked to decreases in histone mRNA.